## HSPA14
- **UniProt:** Q0VDF9 (HSP70L1) · **batch:** proteostasis-batch-2026-06-07b · **review status:** COMPLETE
- **PN placement:** two rows — `Cytonuclear proteostasis|Chaperone|HSP70 system|HSP70` (type) and `Translation|Cytosolic translation|Nascent peptide husbandry|Nascent peptide chaperoning|RAC component` (subtype) ; **PN-node mapping:** HSP70 type → mapped/ok GO:0140662 ATP-dependent protein folding chaperone (more_specific_than_existing_goa); RAC subtype + parent type → mapped/ok GO:0051083 'de novo' cotranslational protein folding (already_in_goa_exact); Translation class/branch → context_only/too_broad (GO:0002181 / GO:0006412).
- **Consistency:** Notes ↔ review ↔ PN RAC row converge — HSPA14 is the atypical Hsp70/DnaK subunit of RAC (heterodimer with DNAJC2/MPP11) acting cotranslationally at the ribosomal exit tunnel; ATPase stimulated by DNAJC2 (PMID:16002468). Notes flag (per MS1) that HSPA14, like yeast Ssz1p, may not be an autonomous foldase — its nucleotide cycle largely serves to stimulate the downstream ribosome-bound HSP70. The cotranslational-folding story is fully and consistently captured.
- **PN story / NEW pressure:** The RAC/cotranslational story is ALREADY captured: review ACCEPTs GO:0051083 'de novo' cotranslational protein folding (TAS) and GO:0101031 protein folding chaperone complex; PN's GO:0051083 is correctly flagged already_in_goa_exact. The HSP70-type GO:0140662 ATP-dependent protein folding chaperone projection is the softer of the two atypical-HSP70 cases: the review itself uses GO:0044183 protein folding chaperone (parent, ACCEPT) as a core MF and MARK_AS_OVER_ANNOTATED only the stress-refolding GO:0042026. So GO:0140662 (ATP-dependent foldase child) is debatable — defensible as RAC engages nascent chains ATP-dependently, but borderline given HSPA14's Ssz1p-like atypia (uncertain intrinsic foldase activity).
- **Mapping strategy:** RAC subtype → GO:0051083 needs no change (exact, already in GOA/review). HSP70-type GO:0140662: narrower than the review's accepted GO:0044183; acceptable but flag the Ssz1p-atypia caveat — if propagated, it should be co-translational-folding-scoped, not stress-refolding. Not a clear-cut rejection like HSPA13.
- **Evidence alignment:** PN rows carry no reference titles; the review's central PMID:16002468 (MPP11/Hsp70L1 form mammalian RAC) underpins both the GO:0051083 and complex annotations — convergent with the PN RAC subtype projection.
- **Verdict:** RAC/cotranslational-folding projection sound and already captured; HSP70-type GO:0140662 is borderline-defensible (atypical Ssz1p-like HSP70) but should carry the caveat. **Recommended edits:** [MAP] keep GO:0051083 for the RAC subtype (already covered); for the HSP70-type GO:0140662 projection, scope to co-translational folding and note HSPA14's Ssz1p-like atypia (intrinsic foldase activity uncertain) rather than asserting canonical ATP-dependent refolding.