## LRSAM1
- **UniProt:** Q6UWE0 (Tal) · **batch:** proteostasis-batch-2026-06-14 · **review status:** COMPLETE (thorough; catalytic RING E3; xenophagy + TSG101/ESCRT; CMT2P; 2 PN rows)
- **PN placement:** row1 `ALP|...|Marking substrates for selective autophagy|Xenophagy|Intracellular pathogen ubiquitination`; row2 `UPS|E3 ubiquitin and UBL ligases|RING|other|LRR`. **PN-node mapping:** row1 Xenophagy type→mapped GO:0098792 xenophagy (subtype contextual no_mapping); row2 RING group→mapped GO:0061630 ubiquitin protein ligase activity (class context_only/too_broad).
- **Consistency:** Excellent. Deep research, review and PN all agree LRSAM1 is a genuine catalytic RING E3 (EC 2.3.2.27) that (a) recognizes cytosolic bacteria via LRRs and generates the bacteria-associated polyUb signal via its RING to drive xenophagy (PMID:23245322), and (b) as Tal monoubiquitinates TSG101 (ESCRT) controlling endocytosis/retroviral budding. PN row2 ("genuine catalytic RING E3") matches the review's ACCEPT of GO:0061630/GO:0004842 as CORE. No contradictions.
- **PN story / NEW pressure:** Row1 projects GO:0098792 xenophagy (verified real; ABSENT from GOA as the base process). PN flags it goa_status=supported_by_goa_regulation — confirmed: GOA carries GO:1904417 positive regulation of xenophagy (IMP, PMID:23245322) but NOT the parent GO:0098792. The review accepts GO:1904417 as core and never adds the base xenophagy term. So GO:0098792 is a defensible **ADD** (the regulation term already in GOA strongly supports it). Row2 GO:0061630 is correctly already_in_goa_exact (EXP/IDA/IEA all present) — keep the catalytic ligase MF as core (matches the KEY PATTERN: LRSAM1 generates the autophagy ubiquitin signal).
- **Mapping strategy:** Correct. Catalytic RING→GO:0061630 (exact, in GOA); Xenophagy type→GO:0098792 (narrower-but-inside the process). The contextual "Intracellular pathogen ubiquitination" subtype is appropriately no_mapping. The xenophagy process projection is consistent with — not broader than — the review's accepted GO:1904417; no TOMM20-style over-reach.
- **Evidence alignment:** Strong. PN row1 ref (LRR+RING LRSAM1 E3 xenophagy of Salmonella = PMID:23245322) is the review's HIGH/VERIFIED anchor. PN row2 "19489725 / rev" is a family-review pointer not in the review.
- **Verdict:** Fully consistent and mutually reinforcing; catalytic ligase MF is core (already in GOA); base xenophagy BP is a justified ADD the review omits (regulation child already curated).
- **Recommended edits:** [YAML] add GO:0098792 xenophagy (BP, involved_in) — the base process is absent though GO:1904417 (its positive-regulation child) is already accepted as core; cite PMID:23245322.
- **2026-06-18 follow-up:** Implemented the YAML edit: added GO:0098792 xenophagy as a NEW recommendation and added the base process to the core xenophagy function while retaining GO:1904417 positive regulation of xenophagy.