## MEFV
- **UniProt:** O15553 (Pyrin/TRIM20) · **batch:** proteostasis-batch-2026-06-14 · **review status:** COMPLETE (very thorough; inflammasome sensor + precision-autophagy receptor; 2 PN rows)
- **PN placement:** row1 `ALP|Autophagy substrate selection|Selective autophagy receptor|Individual substrates`; row2 `UPS|E3 ubiquitin and UBL ligases|RING|TRIM / unclassified|Pyrin, ringless, SPRY`. **PN-node mapping:** row1 type→mapped GO:0160247 autophagy cargo adaptor activity; row2 RING group→mapped GO:0061630 ubiquitin protein ligase activity (class context_only/too_broad).
- **Consistency:** Mostly consistent, with one watch-point the PN mapping itself flags. Deep research, review and PN agree pyrin is both a pro-inflammatory inflammasome sensor (ASC/caspase-1/IL-1β) and an anti-inflammatory TRIM20 "precision-autophagy" receptor degrading NLRP3/NLRP1/pro-caspase-1. The PN row2 subtype label literally says "Pyrin, ringless, SPRY" — i.e. PN itself records that pyrin is a RINGLESS TRIM, matching the review's `MARK_AS_OVER_ANNOTATED` of GO:0061630 (no functional RING). No hard contradiction.
- **PN story / NEW pressure:** Row1 projects GO:0160247 autophagy cargo adaptor activity (verified real; confirmed ABSENT from GOA). This is a defensible **ADD**: pyrin/TRIM20 binds inflammasome cargo and recruits ATG machinery (PMID:26347139), an MF the review currently captures only as positive-regulation-of-autophagy (KEEP_AS_NON_CORE) + bare protein binding — never as cargo-adaptor MF. Row2 GO:0061630 is the over-annotation watch case: PN's RING-group default projects ligase MF, but pyrin has NO functional RING; the review correctly marks the existing IBA GO:0061630 as over-annotated.
- **Mapping strategy:** Row1 cargo-adaptor mapping is sound and aligns with the established CALCOCO/TRIM precision-autophagy receptor concept. Row2 needs a per-gene caveat: the RING-group→GO:0061630 propagation is the right *default* for catalytic RING E3s but MUST be suppressed for pyrin (ringless) — exactly the over-annotation pattern. The subtype is already no_mapping, so propagation flows only from the group default; recommend a node-level exclusion note for ringless members.
- **Evidence alignment:** Good. PN row1 ref (precision autophagy JCB = PMID:26347139) is in the review (HIGH/VERIFIED). PN row2 "33791238 / rev" not in the review (family review pointer); review's pyrin biochemistry rests on the inflammasome/PAAND papers instead.
- **Verdict:** Consistent; GO:0160247 cargo-adaptor is a justified ADD; the RING-group ligase MF must NOT propagate to ringless pyrin (review already over-annotates it).
- **Recommended edits:** [YAML] consider adding GO:0160247 autophagy cargo adaptor activity (MF) for the TRIM20 precision-autophagy receptor role (PMID:26347139) — currently captured only as non-core positive-regulation-of-autophagy. [MAP] annotate the RING-group node (or a ringless-TRIM exclusion) so GO:0061630 does NOT auto-propagate to "Pyrin, ringless, SPRY" members lacking a functional RING; the review's over-annotation flag on GO:0061630 should be honored by the mapping.