## MKRN1
- **UniProt:** Q9UHC7 · **batch:** proteostasis-batch-2026-06-07c · **review status:** COMPLETE
- **PN placement:** two rows — `Translation|Cytosolic translation|Ribosome-associated QC|Ubiquitination` (TR) and `UPS|E3 ubiquitin and UBL ligases|RING|Makorin|C3H1-type ZnF` (UPS). **PN-node mapping:** RQC type→GO:0016567 protein ubiquitination (mapped); RQC group→GO:0006515 protein QC for misfolded/incompletely synthesized proteins (mapped); RING group→GO:0061630 ubiquitin protein ligase activity (mapped); Makorin/ZnF subtype + UPS branch = no_mapping.
- **Consistency:** Strong agreement. Review, notes, deep-research, PN annotation, and PN-node mapping all converge: MKRN1 is a makorin RING E3 ligase (GO:0061630, GO:0016567 — both already in GOA, IDA/IBA) that also acts in poly(A) ribosome-associated QC (PMID:31640799). No contradictions.
- **PN story / NEW pressure:** The RQC story IS the value-add and the review already acted on it — it adds GO:1990116 "ribosome-associated ubiquitin-dependent protein catabolic process" as a **NEW** annotation (IMP, PMID:31640799), verified real and non-obsolete via OLS. This is more specific and more apt than the PN-projected GO:0006515. ADD already done in YAML.
- **Mapping strategy:** No node-mapping change needed. The PN RQC-group projection GO:0006515 is **broader** than the review's GO:1990116 (a descendant capturing ribosome-associated ubiquitin-dependent catabolism). Per the TOMM20/HSPA8/RAB7A precedent the broader class-projection is acceptable as a group-level node mapping (it spans many RQC members), but for MKRN1 specifically the gene-level annotation should remain the more specific GO:1990116, not GO:0006515. GO:0016567 / GO:0061630 projections are exact and already in GOA.
- **Evidence alignment:** PN row 2 cites "19489725 / rev" (a makorin review). The review instead anchors on the primary functional papers PMID:19536131 (p53/p21 E3 activity) and PMID:31640799 (poly(A) RQC) — stronger, primary evidence; the PN review-citation is a reasonable family-level pointer not needed in the gene review.
- **Verdict:** Consistent; NEW GO:1990116 already correctly added (verified). No edits required; keep gene-level RQC annotation at GO:1990116 rather than the broader PN-projected GO:0006515.