## MVB12A
- **UniProt:** Q96EY5 · **batch:** proteostasis-pr-1217 · **review status:** COMPLETE
- **PN placement:** `ALP|...|Sealing of autophagophore membrane|ESCRT-I complex component` and `UPS|Ubiquitin and UBL binding|trafficking|ESCRT-I complex|idiosyncratic Ub binding` ; **PN-node mapping:** ALP leaf `mapped / GO:0000813 ESCRT I complex`; ALP "Sealing" group `mapped / ok_for_propagation / GO:0000045 autophagosome assembly`; UPS nodes all `no_mapping` (class `context_only` GO:0140036). Projected: GO:0000813 (`already_in_goa_exact`), GO:0000045 (`more_specific_than_existing_goa`).
- **Consistency:** Partial tension. GO:0000813 ESCRT I complex is fully consistent (multiply ACCEPTed). But the review MARK_AS_OVER_ANNOTATED for GO:0016236 macroautophagy, stating the ESCRT/autophagy review (PMID:20588296) and the VPS28-interface structural assay (PMID:32424346) do NOT establish MVB12A as a core phagophore-closure factor. PN's `mapped/ok_for_propagation` projection of GO:0000045 autophagosome assembly from the "Sealing" group therefore conflicts with the review's stance.
- **PN story / NEW pressure:** PN asserts MVB12A is involved in autophagophore sealing/autophagosome assembly — a role NOT in MVB12A's GOA and NOT added by the review. The review treats this as over-annotation pending MVB12A-specific phagophore-closure perturbation (the VPS37A evidence in PMID:31519728 is about a different ESCRT-I subunit). Conclusion: PN over-reaches at the gene level for GO:0000045; better left as a curator question, not a propagated annotation.
- **Mapping strategy:** This gene argues the "Sealing of autophagophore membrane" group's `ok_for_propagation` (GO:0000045) is too aggressive for MVB12A specifically — the subunit rides in via complex membership, not demonstrated phagophore-closure function (cf. broader-term rejections). Recommend the node treat per-subunit projection of GO:0000045 as candidate, not automatic.
- **Evidence alignment:** Shared paper: PMID:32424346 (ESCRT-I helical assembly) is both the PN reference and the review's source for ESCRT-I/membrane-fission. PN's autophagy framing leans on PMID:20588296; review cites the same but downgrades it. Strong overlap on the structural evidence, divergence on the autophagy inference.
- **Verdict:** ESCRT-I membership consistent; PN's GO:0000045 autophagosome-assembly projection over-reaches per the review's own MARK_AS_OVER_ANNOTATED. **Recommended edits:** none to the gene YAML; flag the "Sealing" node so GO:0000045 is not auto-propagated to MVB12A without subunit-specific closure evidence.