## NAA25
- **UniProt:** Q14CX7 · **batch:** proteostasis-batch-2026-06-07c · **review status:** COMPLETE
- **PN placement:** `Translation|Cytosolic translation|Nascent peptide husbandry|N-terminal acetylation of nascent peptide|NatB complex component` (TR only). **PN-node mapping:** subtype→GO:0031416 NatB complex (mapped, CC); type→GO:0006474 N-terminal protein amino acid acetylation (mapped, BP); group no_mapping; class/branch context_only.
- **Consistency:** Strong agreement. Review, notes, and PN concur NAA25 is the **auxiliary, non-catalytic** subunit of NatB (catalytic subunit is NAA20, not NAA25): NatB complex (GO:0031416, IBA/IPI/ComplexPortal accepted), activator activity (GO:0010698 IBA accepted), cytoplasm. Critically the PN subtype is correctly **NatB** (GO:0031416), not NatA — distinct from NAA10/NAA15. No contradictions.
- **PN story / NEW pressure:** Modest. The PN BP GO:0006474 is flagged new_to_goa for NAA25 (NAA25's GOA has GO:0031416, GO:0010698, GO:0007010, localizations, GO:0005515 — but no GO:0006474). So "N-terminal protein amino acid acetylation" as a process annotation would be genuinely new to NAA25's GOA. It is defensible as process participation (NAA25 is required for NatB activity), but for a non-catalytic scaffold the review prefers the activator MF + complex CC. Verdict: borderline ADD vs already-captured-by-proxy; lean **already captured** via GO:0010698 + GO:0031416, since GO:0006474 risks implying catalytic involvement by the auxiliary subunit.
- **Mapping strategy:** GO:0031416 NatB complex (CC) is exact and the correct shared term — properly distinguishes NAA25 (NatB) from the NAA10/NAA15 NatA node. The cytoskeleton-organization IBA (GO:0007010, from yeast/fly NatB substrate acetylation) is correctly KEEP_AS_NON_CORE; the PN does not project it. No node change needed.
- **Evidence alignment:** PN row carries no reference titles. Review anchors on PMID:18570629 (hNatB identification, VERIFIED) and PMID:34230638 (NAA20 disease variants, VERIFIED, contextual). Good overlap with the primary NatB literature; no divergence.
- **Verdict:** Consistent; auxiliary NatB-subunit identity correct and properly distinguished from NatA. No edits. The PN GO:0006474 BP projection is new-to-GOA but optional — the activator MF + NatB CC already convey the function without over-attributing catalysis.