## NAA35
- **UniProt:** Q5VZE5 · **batch:** proteostasis-batch-2026-06-07c · **review status:** COMPLETE
- **PN placement:** `Translation|Cytosolic translation|Nascent peptide husbandry|N-terminal acetylation of nascent peptide|NatC complex component` ; **PN-node mapping:** subtype mapped→GO:0031417 NatC complex (ok_for_propagation); type mapped→GO:0006474 N-terminal protein amino acid acetylation (ok_for_propagation, new_to_goa)
- **Consistency:** Strong. All three sources agree NAA35 (MAK10/EGAP) is the large **non-catalytic auxiliary/scaffold** subunit of NatC; no catalytic MF is asserted (correct). PN-node mapping (NatC complex CC; Nt-acetylation BP) is consistent with the auxiliary role.
- **PN story / NEW pressure:** PN projects BP GO:0006474 (verified real; confirmed absent from NAA35 GOA). For a non-catalytic scaffold an `involved_in` BP of N-terminal acetylation is defensible (it is part of the complex that performs the process), and complements the review's CC-only core. Conclusion: **ADD GO:0006474 (involved_in)** is reasonable; the catalytic MF must NOT be added (NAA35 is non-catalytic). Not an over-reach.
- **Mapping strategy:** Correct. Subtype→NatC complex exact CC is the safe target for an auxiliary subunit; BP propagated at type level. No node-mapping change warranted.
- **Evidence alignment:** Concordant. Review/notes use PMID:19398576 (NatC; hMak10 auxiliary; IDA GO:0031417, IMP GO:0043066), PMID:37891180, plus mouse EGAP origin (PMID:16484612, cited in notes) for the smooth-muscle/vascular ISS/IEA. PN row carries no titles. No divergence.
- **Verdict:** Consistent, high-quality. Internal note vs YAML mismatch: notes say PMID:32296183 (TRIM7) protein-binding should be MARK_AS_OVER_ANNOTATED, but YAML uses KEEP_AS_NON_CORE (minor, defensible either way). **Recommended edits:** Add BP GO:0006474 (involved_in, auxiliary subunit of the acetylating complex) to NAA35 review [YAML]; optionally reconcile PMID:32296183 action with notes (KEEP_AS_NON_CORE vs MARK_AS_OVER_ANNOTATED) [YAML].
- **2026-06-18 follow-up:** Implemented the high-confidence YAML edit: added GO:0006474 N-terminal protein amino acid acetylation as a NEW BP recommendation and core process for the NatC auxiliary subunit, using IC to reflect inference from NatC complex membership rather than direct catalytic activity. The optional protein-binding action-style reconciliation remains separate.