## NLRX1
- **UniProt:** Q86UT6 (NOD5/NOD9) · **batch:** proteostasis-batch-2026-06-14 · **review status:** COMPLETE (thorough; mitochondrial NLR; MAVS/RIG-I, TUFM-autophagy, NF-κB direction disputed)
- **PN placement:** `Autophagy-Lysosome Pathway|Autophagy substrate selection|Selective autophagy receptor|Mitophagy` ; **PN-node mapping:** type→mapped GO:0000423 mitophagy (ancestors no_mapping containers).
- **Consistency:** Partial mismatch on the autophagy framing. Deep research and review converge on NLRX1 as a mitochondrial-outer-membrane regulator: core = negative regulation of MAVS/RIG-I antiviral signaling; secondary = TUFM-dependent PROMOTION of autophagy (recruits ATG5-ATG12/ATG16L1); plus NLRP3-inflammasome restraint and a DISPUTED NF-κB direction (UniProt: enhances via ROS; IBA: negative). The PN places NLRX1 specifically as a "Mitophagy" selective-autophagy RECEPTOR with a LIR motif (Listeria-induced mitophagy, Nat Immunol). The review's notes/YAML do NOT describe NLRX1 as a LIR-bearing mitophagy receptor — they describe TUFM-mediated general autophagy and never cite the Listeria-mitophagy paper. So the PN's mitophagy-receptor story is not reflected in the review.
- **PN story / NEW pressure:** PN projects GO:0000423 mitophagy as new_to_goa (verified real; confirmed ABSENT from NLRX1 GOA — GOA has no autophagy/mitophagy BP at all). The review independently proposes a NEW positive-regulation-of-autophagy term (GO:0010508) from the TUFM mechanism, but does NOT propose mitophagy. These are two different autophagy claims resting on different evidence: review = TUFM/general autophagy (PMID:22749352); PN = LIR-dependent Listeria mitophagy (PN-cited Nat Immunol paper not in the review). NEW autophagy pressure is real either way; the specific MITOPHAGY assertion needs the PN's mitophagy-receptor paper to be verified before adding.
- **Mapping strategy:** The mitophagy leaf→GO:0000423 propagation is internally reasonable for a bona fide mitophagy receptor, but for NLRX1 it hinges on a single PN reference (Listeria mitophagy receptor) that the gene review did not adjudicate. Status `mapped` may over-reach for human NLRX1 if the LIR/mitophagy-receptor role is mouse/infection-specific; recommend confirming before propagating mitophagy as a universal NLRX1 GO assertion. The disputed-NF-κB issue is not in the PN mapping (PN is autophagy-only) so no conflict there.
- **Evidence alignment:** Divergent. PN refs = a selective-autophagy/LIR review + "Listeria hijacks host mitophagy" (Nat Immunol) — NEITHER is in the review's reference list. The review's autophagy evidence is PMID:22749352 (NLRX1-TUFM) + PMID:28611246 (TUFM/influenza), which the PN does not cite. This is the most salient divergence.
- **Verdict:** Consistent on antiviral core; the PN mitophagy-receptor story is NOT captured in the review and rests on a paper the review never assessed — verify before treating GO:0000423 as a settled ADD.
- **Recommended edits:** [REF/WB] obtain and assess the PN-cited "Listeria hijacks host mitophagy through a novel mitophagy receptor" (Nat Immunol) — it is absent from the review; it is the sole basis for the NLRX1 LIR/mitophagy-receptor claim and for GO:0000423. [YAML] if verified, add GO:0000423 mitophagy (and reconcile with the review's existing GO:0010508 proposed_new_term, which currently frames the autophagy role generically via TUFM rather than as mitophagy).