## OTUD3
- **UniProt:** Q5T2D3 · **batch:** proteostasis-batch-2026-06-07c · **review status:** COMPLETE (rich, 40 annotations, all ACCEPT/KEEP_AS_NON_CORE)
- **PN placement:** 3 rows — `Translation|Cytosolic translation|Ribosome-associated QC|Deubiquitination`; `UPS|DUBs and UBL demodifiers|OTU|other`; `UPS|Ubiquitin and UBL binding|DUB|OTU|UBA-like (other)`. **PN-node mapping:** RQC-type→mapped GO:0101005 deubiquitinase activity; RQC-group→mapped GO:0006515 protein QC (new_to_goa); UPS OTU/UBL nodes mostly no_mapping/context_only. Projected: GO:0006515 (new), GO:0101005 (entailed).
- **Consistency:** Strong. Deep research, review, and PN all agree OTUD3 is an OTU-family Cys-protease DUB (Cys76) with K6/K11 preference; the RQC role (deubiquitinating 40S eS10/uS10, antagonizing ZNF598; PMID:32011234) is captured in description + IDA annotations. No contradictions. Nuance: review/description frame the RQC role as **negative** regulation (a brake on RQC); PN files OTUD3 as an RQC "Deubiquitination" member, which is correct directionally but the PN-group→GO:0006515 "protein quality control" projection slightly overstates OTUD3's role (it antagonizes, not drives, QC).
- **PN story / NEW pressure:** GO:0101005 deubiquitinase activity (verified real) is entailed by the existing GO:0004843 cysteine-type DUB activity already annotated (IDA/IBA/TAS) — already captured, no NEW pressure on MF. GO:0006515 (verified real) is new_to_goa and absent from review; given OTUD3 antagonizes RQC, a positive "involved_in protein QC" assertion over-reaches. Conclude: MF already captured; process term over-reaches as a propagation.
- **Mapping strategy:** Does not change the node. RQC-type→GO:0101005 redundant with the review's GO:0004843. The group→GO:0006515 propagation is the only divergence and should NOT be projected to OTUD3 (antagonist).
- **Evidence alignment:** PN cites only PMID:23827681 (OTU linkage-specificity); review has it (HIGH/VERIFIED) plus the full substrate literature (26280536 PTEN, 32011234 RQC, 35675826 PPARD, 38288086 KPTN). Review strictly superset; no divergence.
- **Verdict:** Consistent; PN MF already captured, PN RQC-process projection (GO:0006515) over-reaches because OTUD3 is a negative regulator of RQC. No YAML change needed.
- **Recommended edits:** [MAP] Do not propagate GO:0006515 (protein QC) to OTUD3 from the RQC-group node — OTUD3 antagonizes ZNF598-driven 40S ubiquitination (negative RQC regulator), so an `involved_in protein quality control` assertion mis-states direction; flag as do-not-project.