## PDCD6IP
- **UniProt:** Q8WUM4 · **batch:** proteostasis-pr-1217 · **review status:** COMPLETE
- **PN placement:** `ALP|Autophagosome closure maturation and lysosome fusion|Sealing of autophagophore membrane|Localization of the ESCRT-III complex` ; **PN-node mapping:** leaf `no_mapping` (members mix endosomal-sorting + SNARE genes); "Sealing" group `mapped / ok_for_propagation / GO:0000045 autophagosome assembly`; class `context_only` GO:0016236. Projected: GO:0000045 (`more_specific_than_existing_goa`).
- **Consistency:** Largely consistent. The PN note (ALIX/ATG12-ATG3 recruits ESCRT-III for autophagosome closure) aligns with the review, which ACCEPTs GO:0016236 macroautophagy (ISS) on the strength of PMID:25686249 (ALIX required for basal autophagic flux). The autophagy-closure framing is supported. The leaf being `no_mapping` (heterogeneous members) is consistent with the review not asserting an autophagy-specific ESCRT-III-localization MF for ALIX.
- **PN story / NEW pressure:** PN projects GO:0000045 autophagosome assembly (`more_specific_than_existing_goa` — GOA/review have the broader GO:0016236 macroautophagy). For ALIX this is more defensible than for the MVB12 subunits because the gene-specific basal-autophagy evidence (PMID:25686249) exists, but the cited support is for basal autophagic flux/late-endosome function rather than phagophore sealing specifically. Conclusion: GO:0000045 is a plausible candidate (not pure over-reach), but the review's macroautophagy ACCEPT already captures the role; adding autophagosome assembly should be expert-gated, not auto-propagated.
- **Mapping strategy:** Node scoping is sound. The `no_mapping` leaf correctly avoids over-claiming; the "Sealing" group projecting GO:0000045 is acceptable as candidate for ALIX given basal-autophagy evidence, but should remain `more_specific` candidate rather than displacing the curated macroautophagy call.
- **Evidence alignment:** Shared: PMID:25686249 (ATG12-ATG3–ALIX) is a PN reference and the review's macroautophagy anchor. PN also cites closure-regulator and exosome/autophagy reviews; review independently supports MVB/exosome/cytokinesis (PMID:12860994, PMID:22660413, PMID:18641129). Good overlap on the autophagy link.
- **Verdict:** Consistent; PN autophagosome-assembly projection is a reasonable candidate but the curated macroautophagy ACCEPT already covers it. No edits required.