## PIK3R4
- **UniProt:** Q99570 · **batch:** proteostasis-pr-1217 · **review status:** COMPLETE
- **PN placement:** `ALP|Autophagophore initiation and elongation|Class 3 PI3K complex 1, direct|Class 3 PI3K complex 1 component` (row 1) and `ALP|Autophagosome closure maturation and lysosome fusion|Class 3 PI3K complex 2, direct|Class 3 PI3K complex 2 component` (row 2) ; **PN-node mapping:** both `type` leaves mapped, `ok_for_propagation_to_go` → GO:0034271 (C1/type I) and GO:0034272 (C2/type II); `group`/`class`/`branch` ancestors all context_only/no_mapping.
- **Consistency:** Strong. Notes, review YAML, and PN all model PIK3R4/VPS15 as the regulatory/scaffolding subunit of class III PI3K complexes (PI3KC3-C1 and -C2), not the catalytic kinase. Review description and core_functions name both complexes explicitly. No contradictions.
- **PN story / NEW pressure:** Already captured. PN asserts membership in both class III complexes; review ACCEPTs GO:0034271 and GO:0034272 (both already IBA in GOA — PN goa_status `already_in_goa_exact`). No NEW GO term needed. Verdict: already captured.
- **Mapping strategy:** Correct and unchanged by this gene. The two `type`-leaf mappings to GO:0034271/GO:0034272 are the right grain; ancestors (GO:0035032 class III PI3K complex; GO:0016236 macroautophagy) are correctly held as too_broad_to_propagate, consistent with the TRAPP-overpropagation precedent. PN-projected terms are neither broader nor narrower than the review — they coincide.
- **Evidence alignment:** Partial overlap. PN cites review-style sources (Annual Review; Yang & Klionsky; PMID:19270696 Matsunaga Atg14L/Rubicon Nat Cell Biol). Review uses primary literature: PMID:8999962, 25490155, 40442316 (PI3KC3-C1 structure), 19270696, 20643123, 14617358. PMID:19270696 is shared. Review also flags VPS15 as a pseudokinase (PMID:40442316) and REMOVEs yeast nucleus-vacuole junction, MODIFYs pexophagy→autophagosome assembly — finer than PN.
- **Verdict:** Consistent; PN complex membership fully captured by existing GOA + review ACCEPT. No new GO pressure; mapping grain correct. No edits required.