## RACK1
- **UniProt:** P63244 · **batch:** proteostasis-batch-2026-06-07c · **review status:** COMPLETE (very large, ~80 annotations; core = 40S/RQC scaffold)
- **PN placement:** 3 rows — `Translation|Cytosolic translation|Ribosome biogenesis factor|pre-40S complex`; `…|Ribosome-associated QC|Ribosomal rescue`; `Autophagy-Lysosome Pathway|…|Modulation of ATG5-ATG12-ATG16 complex activity`. **PN-node mapping:** biogenesis-group→mapped GO:0042254 ribosome biogenesis (new_to_goa); pre-40S-type→mapped GO:0030688 preribosome SSU precursor (new_to_goa); rescue-type→GO:0072344 (already_in_goa); RQC-group→GO:0006515 (new); ALP leaf/group no_mapping. Projected: GO:0042254, GO:0030688, GO:0006515 (all new), GO:0072344 (in GOA).
- **Consistency:** Partial. Review/notes and PN agree strongly on the **40S structural + RQC scaffold** core (integral 40S protein, collision sensing, ZNF598-dependent RPS2/3/20 ubiquitylation, GO:0072344, GO:0016567, GO:0043022). **Two PN rows are absent from the review/notes:** (a) ribosome **biogenesis / pre-40S** placement, and (b) the **ATG5/autophagy** regulator role (PN Notes cite the RACK1-ATG5 paper). GOA confirms NO biogenesis, pre-40S, GO:0042254, GO:0030688, GO:0016236 or ATG terms. So PN asserts two roles the review neither covers nor refutes.
- **PN story / NEW pressure:** GO:0042254 ribosome biogenesis and GO:0030688 preribosome SSU precursor (both verified real) are genuinely new and NOT in review/GOA. RACK1 is incorporated late into pre-40S particles, so a biogenesis/pre-40S association is biologically plausible — but the review's stance is that RACK1's ribosomal role is **structural/translational/RQC**, not assembly. This is a candidate ADD that needs literature support before projection (the review did not assess it). GO:0072344 already captured (IMP+IBA). GO:0006515 broader/redundant with GO:0072344.
- **Mapping strategy:** RACK1 is a ribosomal scaffold/collision sensor, not a biogenesis factor by the review's framing; projecting GO:0042254/GO:0030688 from the pre-40S node risks the TRAPP-like/biogenesis over-propagation the dossier itself warns about. Recommend these stay gene-curated, not auto-projected, until verified. RQC-group→GO:0006515 broader than the exact GO:0072344 already present.
- **Evidence alignment:** PN row3 cites the RACK1-ATG5 autophagy paper (title only, no PMID); this paper is absent from the review references entirely. PN biogenesis rows cite nothing. Review RQC evidence (PMID:28132843, 23636399, 25901680) does not overlap PN's cited material. Divergence: autophagy + biogenesis literature uncited in review.
- **Verdict:** Core RQC/40S consistent; PN biogenesis (GO:0042254/GO:0030688) and ATG5-autophagy stories are NOT in the review — candidate ADDs requiring verification, currently over-reaching as auto-projections.
- **Recommended edits:** [YAML] Consider adding a note/annotation on the RACK1-ATG5 autophagy-regulator role (PN-cited) and the pre-40S/biogenesis association if literature supports — both are absent from the current review. [MAP] Do NOT auto-project GO:0042254/GO:0030688 to RACK1 from the biogenesis/pre-40S node without gene-level evidence (RACK1 framed as structural/RQC scaffold, not an assembly factor); GO:0006515 broader than the exact GO:0072344 already present. [REF] RACK1-ATG5 paper (resolve to a PMID) missing from review references.