## RNF14
- **UniProt:** Q9UBS8 · **batch:** proteostasis-batch-2026-06-07c · **review status:** COMPLETE (thorough; core ligase + RQC, AR/Wnt as non-core moonlighting)
- **PN placement:** 3 rows — `Translation|Cytosolic translation|Ribosome-associated QC|ubiquitination of eEF1A on stalled ribosomes`; `UPS|E3 ubiquitin and UBL ligases|RBR|RWD`; `UPS|Ubiquitin and UBL binding|E3 ligase|RBR / with UBD|RWD`. **PN-node mapping:** RQC-type→mapped GO:0016567 protein ubiquitination (already_in_goa); RQC-group→GO:0006515 (new); RBR-group→mapped GO:0061630 ubiquitin protein ligase activity (already_in_goa); UBL-binding E3-group→GO:0061630 (already_in_goa); RBR/RWD subtype/type no_mapping (covered by parent). Projected: GO:0006515 (new), GO:0016567, GO:0061630×2 (all in GOA).
- **Consistency:** Excellent. Deep research, review and PN concur: RBR-type E3 (RWD + TRIAD/RING1-IBR-RING2), GCN1-recruited, K6-linked ubiquitination of eEF1A/eRF1 on stalled ribosomes, plus reactive-aldehyde RNA-protein crosslink resolution (GO:0160127), and the AR/Wnt nuclear moonlighting role (kept non-core). The PN correctly distinguishes the **catalytic ligase MF** (GO:0061630 at the RBR group) from process. No contradictions.
- **PN story / NEW pressure:** GO:0061630 (verified real), GO:0016567 — both already in GOA + review (multiple IDA, Cys-220/Cys-417 mutagenesis). GO:0006515 (verified real) is new_to_goa; RNF14 genuinely acts in stalled-ribosome QC so it is a defensible higher-level ADD, but broader than the review's precise set (GO:0072344, GO:0085020 K6, GO:0160127, GO:0006511). Conclude: ligase MF + ubiquitination already captured; GO:0006515 defensible-but-broader ADD.
- **Mapping strategy:** Correctly resolves RNF14 as the catalytic ligase (GO:0061630), not a generic UBD reader — group-level mapping appropriate; subtype/type no_mapping avoids double-counting. RQC-group→GO:0006515 broader than terms already present. No node change warranted.
- **Evidence alignment:** PN RBR row cites "PMID 17367545 / rev" — this PMID is NOT in the review's references. Review's RQC evidence (PMID:36638793, 37651229, 37951215/16, 27863242) is HIGH/VERIFIED and richer than the PN row. Divergence: the single PN-cited RBR PMID is uncited in the review (likely an older RBR-family review; low impact since ligase MF is independently well-supported).
- **Verdict:** Fully consistent; PN ligase MF (GO:0061630) and ubiquitination already captured, GO:0006515 a defensible-but-broader ADD. No YAML change needed.
- **Recommended edits:** none required. [REF] (optional) check PN-cited PMID:17367545 (RBR row) — absent from review; verify whether it adds RNF14-specific support or is a family review. [MAP] GO:0006515 broader than the exact RQC terms already annotated.