## RNF25
- **UniProt:** Q96BH1 · **batch:** proteostasis-batch-2026-06-07c · **review status:** COMPLETE (thorough; core RING ligase + RQC, AO7/NF-kB as non-core)
- **PN placement:** 3 rows — `Translation|Cytosolic translation|Ribosome-associated QC|ubiquitination of eEF1A on stalled ribosomes`; `UPS|E3 ubiquitin and UBL ligases|RING|ubiquitin binding domain|RWD`; `UPS|Ubiquitin and UBL binding|E3 ligase|RING / with UBD|RWD`. **PN-node mapping:** RQC-type→mapped GO:0016567 protein ubiquitination (already_in_goa); RQC-group→GO:0006515 (new); RING-group→mapped GO:0061630 ubiquitin protein ligase activity (already_in_goa); UBL-binding E3-group→GO:0061630 (already_in_goa); RING subtype/type no_mapping. Projected: GO:0006515 (new), GO:0016567, GO:0061630×2 (all in GOA).
- **Consistency:** Excellent. Deep research, review and PN concur: RING-H2 E3 (RWD for E2 recognition; works with UBE2D2/UbcH5B), the partner ligase of RNF14 in the RNF14-RNF25 pathway — ubiquitinates RPS27A/eS31 on collided ribosomes to activate RNF14, ubiquitinates eRF1/ribosomal proteins, and assembles K6 chains on formaldehyde RNA-protein crosslinks (GO:0160127); legacy AO7/NF-kB(RELA) role kept non-core. PN correctly tags the **catalytic ligase MF**. No contradictions.
- **PN story / NEW pressure:** GO:0061630 (verified real) and GO:0016567 already in GOA + review (IDA PMID:36638793, 26475854, 37651229, 37951215). GO:0006515 (verified real) is new_to_goa; RNF25 genuinely drives stalled-ribosome QC so it is a defensible higher-level ADD, but broader than the review's precise set (GO:0072344, GO:0085020 K6, GO:0160127, GO:0006511). Conclude: ligase MF + ubiquitination already captured; GO:0006515 defensible-but-broader ADD.
- **Mapping strategy:** Correctly resolves RNF25 as catalytic RING ligase (GO:0061630), distinct from a UBD reader; group-level mapping appropriate, subtype/type no_mapping avoids double-counting. RQC-group→GO:0006515 broader than terms already present. No node change warranted.
- **Evidence alignment:** PN RING row cites "PMID 19489725 / rev" — NOT in the review references. Review RQC/ligase evidence (PMID:36638793, 26475854, 37651229, 37951215/16, 27863242, 12748188) is HIGH/VERIFIED and far richer. Divergence: the single PN-cited RING PMID is uncited in the review (likely an older RING/E3 review; low impact, ligase MF independently well-supported).
- **Verdict:** Fully consistent; PN ligase MF (GO:0061630) and ubiquitination already captured, GO:0006515 a defensible-but-broader ADD. No YAML change needed.
- **Recommended edits:** none required. [REF] (optional) check PN-cited PMID:19489725 (RING row) — absent from review; verify whether it adds RNF25-specific support or is a family review. [MAP] GO:0006515 broader than the exact RQC terms already annotated.