## RNF41
- **UniProt:** Q9H4P4 (NRDP1/FLRF) · **batch:** proteostasis-batch-2026-06-14 · **review status:** COMPLETE (very thorough; catalytic RING E3; ErbB3/ErbB4, BRUCE, Parkin substrates; mitophagy complex; 2 PN rows)
- **PN placement:** row1 `ALP|...|Marking substrates for selective autophagy|Mitophagy|PINK/PRKN pathway`; row2 `UPS|E3 ubiquitin and UBL ligases|RING|SIAH / SINA`. **PN-node mapping:** row1 Mitophagy type→mapped GO:0000423 mitophagy (PINK/PRKN subtype no_mapping); row2 RING group→mapped GO:0061630 ubiquitin protein ligase activity (class context_only/too_broad).
- **Consistency:** Mostly consistent with a coverage gap. Deep research, review and PN agree RNF41 is a genuine catalytic RING E3 (Cys34/His36/Asp56; RING mutations abolish activity) degrading ErbB3/ErbB4, BRUCE/BIRC6 (→apoptosis) and Parkin/PRKN (→↑ROS). The PN row1 mitophagy story = "CLEC16A-RNF41-USP8 complex maintains mitochondrial function / late mitophagy; also ubiquitinates BIRC6 to upregulate autophagy" (tandfonline beta-cell paper). The review NOTES the RNF41-PRKN/CLEC16A late-mitophagy role in `description` and a suggested_question, but the cited beta-cell mitophagy paper is NOT in the review's references and there is NO mitophagy/autophagy BP annotation. So the PN's mitophagy assertion is mentioned but not evidenced or annotated in the review.
- **PN story / NEW pressure:** Row1 projects GO:0000423 mitophagy as new_to_goa (verified real; confirmed ABSENT from GOA — RNF41 GOA has ligase MF/polyUb/proteasomal-catabolism but no mitophagy/autophagy BP). This is a candidate **ADD** but rests on the CLEC16A-RNF41-USP8 complex / late-mitophagy mechanism whose primary paper the review did not assess (UniProt cites PMID:24949970, not cached). Verify before adding. Row2 GO:0061630 = already_in_goa_exact — keep catalytic ligase MF as core (KEY PATTERN; RNF41 is unambiguously a catalytic RING E3, review ACCEPTs).
- **Mapping strategy:** Row2 catalytic-RING→GO:0061630 is correct (exact, in GOA, multiple IDA). Row1 mitophagy leaf→GO:0000423: defensible in principle (RNF41-PRKN-CLEC16A is a real mitophagy module) but the PINK/PRKN subtype is no_mapping and the propagation flows from the Mitophagy-type node; for RNF41 the role is regulatory/late-stage (autophagosome-lysosome fusion) rather than RNF41 being a cargo-marking receptor, so "Marking substrates for selective autophagy" placement is a slightly loose fit. Status `mapped` is acceptable if the late-mitophagy evidence is confirmed.
- **Evidence alignment:** Divergent on the mitophagy arm. PN row1 ref (beta-cell ubiquitin-dependent mitophagy complex, tandfonline) is NOT in the review. Review's catalytic/substrate evidence (PMID:18541373 Parkin, PMID:14765125 BRUCE, PMID:11867753/27353365 ErbB3, PMID:15314180 USP8) is rich but covers the UPS/ligase side, not the mitophagy paper. PN row2 "19489725 / rev" is a family-review pointer.
- **Verdict:** Consistent on catalytic ligase core (in GOA); the mitophagy ADD is plausible but the PN's primary mitophagy reference is absent from the review — verify before treating GO:0000423 as settled.
- **Recommended edits:** [REF/WB] obtain/assess the PN-cited CLEC16A-RNF41-USP8 beta-cell mitophagy paper (and UniProt's PMID:24949970 late-mitophagy citation) — both absent from the review; they are the basis for GO:0000423. [YAML] if verified, add GO:0000423 mitophagy (regulation-of-mitophagy may fit better given RNF41's late/fusion-stage role) — currently the mitophagy role is mentioned in `description` but unannotated and uncited.