## TCF25
- **UniProt:** Q9BQ70 · **batch:** proteostasis-batch-2026-06-07c · **review status:** COMPLETE (clean; core = RQC complex K48-chain cofactor, Nulp1 legacy non-core)
- **PN placement:** 1 row — `Translation|Cytosolic translation|Ribosome-associated QC|other RQC processes`. **PN-node mapping:** RQC-type ("other RQC processes")→no_mapping (heterogeneous bucket); RQC-group→mapped GO:0006515 protein QC (new_to_goa); class/branch context_only. Projected: GO:0006515 (new) only.
- **Consistency:** Excellent. Deep research/notes, review and PN concur: TCF25 (= yeast Rqc1 ortholog; legacy Nulp1/hnulp1) is an accessory subunit of the 60S RQC complex (LTN1 + NEMF + TCF25), required to promote K48-linked polyubiquitin chains during LTN1-mediated nascent-chain ubiquitination (PMID:30244831). The legacy bHLH "transcription factor"/XIAP/cell-death role is correctly demoted to non-core. PN's "other RQC processes" no_mapping is consistent with the review treating TCF25 as a cofactor/scaffold without a clean own-MF. No contradictions.
- **PN story / NEW pressure:** GO:0006515 (verified real) is new_to_goa and absent from the review. The review instead uses GO:1990116 ribosome-associated ubiquitin-dependent protein catabolic process (NAS), GO:0061945 regulation of protein K48-linked ubiquitination (IDA), GO:0072344 (NAS/IDA) and GO:1990112 RQC complex (IDA/IBA/NAS). Verified via OLS: GO:1990116 sits in the **catabolic-process** branch (under GO:0006511), NOT under GO:0006515 — so GO:0006515 is a related but distinct higher-level QC-process term, a defensible ADD that better matches the canonical RQC framing. Conclude: function captured (via GO:0061945/GO:1990116/GO:1990112); GO:0006515 a reasonable additional/parent QC-process ADD.
- **Mapping strategy:** Sound. The leaf "other RQC processes"→no_mapping (heterogeneous) is the right call; the only projection comes from the RQC-group→GO:0006515. TCF25 genuinely participates in protein QC of incompletely synthesized proteins, so GO:0006515 is appropriately scoped (not over-broad here, since TCF25 has no competing finer own-process term beyond the complex/regulation annotations). No node change.
- **Evidence alignment:** PN row cites no PMIDs. Review's key evidence PMID:30244831 (K48 linkage role) + PMID:35452614 (RQC review, ComplexPortal NAS) is HIGH/VERIFIED. The 14 IPI protein-binding partners are all high-throughput, correctly non-core. No divergence.
- **Verdict:** Fully consistent; function captured via GO:1990112/GO:0061945/GO:1990116; PN's GO:0006515 is a defensible additional QC-process ADD (distinct from the catabolic-branch GO:1990116). No YAML change needed.
- **Recommended edits:** none required. [YAML] (optional) GO:0006515 protein quality control for misfolded or incompletely synthesized proteins could be added as an `involved_in` process term to better surface TCF25's RQC role (currently only catabolic/regulation/complex terms present); supported by PMID:30244831.