## TRIM13
- **UniProt:** O60858 (RFP2/Leu5) · **batch:** proteostasis-batch-2026-06-14 · **review status:** COMPLETE (thorough)
- **PN placement:** 3 rows — `ER proteostasis|Organelle-specific protein degradation|ER associated degradation|Cytosolic handling of ERAD substrates|ERAD-associated RING E3 ligase`; `ALP|...|Marking substrates for selective autophagy|ERphagy|Ubiquitination of ER proteins`; `UPS|E3 ubiquitin and UBL ligases|RING|TRIM / class XI|transmembrane`. **PN-node mapping:** ERAD group → mapped/exact GO:0036503 ERAD pathway (already_in_goa_exact); ERAD subtype RING → mapped GO:0061630 (already_in_goa_exact); ERphagy type+subtype → mapped/ok GO:0061709 reticulophagy (new_to_goa, x2); RING group → GO:0061630 (already_in_goa_exact).
- **Consistency:** Strongly consistent. PN (ER-membrane RING E3 ligase in ERAD + ERphagy marker), review, and notes all agree: TRIM13 is a single-pass ER-membrane RING E3 (functional RING, PMID:17314412) working with VCP/p97 in ERAD, and a positive regulator of ER-stress-induced autophagy/reticulophagy colocalizing with SQSTM1/ZFYVE1 (PMID:22178386). Genuine catalytic RING → catalytic ligase MF is correct. No contradictions.
- **PN story / NEW pressure:** Two stories: (1) ERAD/RING-ligase — already_in_goa_exact (GOA has GO:0036503 ERAD IDA+IBA and GO:0061630/0004842 ligase) → already captured. (2) ERphagy/reticulophagy — GO:0061709 reticulophagy verified real and confirmed NOT in GOA (GOA has only ERAD). The review captures this as GO:0016239 positive regulation of macroautophagy (ACCEPT, IDA PMID:22178386), so the autophagy role is partly captured but at a less specific altitude than reticulophagy. Defensible ADD: GO:0061709 reticulophagy (or regulation thereof, GO:0140500). Note TRIM13's reticulophagy evidence is "positively regulates ER-stress autophagy" → `regulation of reticulophagy` (GO:0140500, verified real) may fit better than the bare process.
- **Mapping strategy:** No node change needed. ERAD=exact and RING=exact are correct (catalytic RING, genuinely ERAD). The reticulophagy projection is appropriately new_to_goa; consider whether the PN should project `regulation of reticulophagy` given the evidence is regulatory rather than TRIM13-as-receptor.
- **Evidence alignment:** Strong overlap. PN ERphagy titles (Regulatory events controlling ER-phagy; N-Degron Pathway Mediates ER-phagy) are reviews; the review's reticulophagy anchor is the experimental PMID:22178386. RING-node refs 33791238/19489725 are TRIM/E3 reviews. Review refs (PMID:17314412, 22178386, 21333377, 25152375) cover ERAD + autophagy + substrate + NF-kB arms.
- **Verdict:** Consistent; ERAD/RING already captured; reticulophagy ADD warranted (regulatory framing preferable). **Recommended edits:** [YAML] add GO:0061709 reticulophagy (or GO:0140500 regulation of reticulophagy, matching the regulatory evidence) involved_in, supported by PMID:22178386 — currently new_to_goa and more specific than the existing GO:0016239.