## TRIM16
- **UniProt:** O95361 (EBBP) · **batch:** proteostasis-batch-2026-06-14 · **review status:** COMPLETE (thorough)
- **PN placement:** 2 rows — `ALP|Autophagy substrate selection|Selective autophagy receptor|Lysophagy`; `UPS|E3 ubiquitin and UBL ligases|RING|TRIM / unclassified|ringless & SPRY`. **PN-node mapping:** Lysophagy type → mapped/ok GO:0160247 autophagy cargo adaptor activity (new_to_goa); RING group → mapped/ok GO:0061630 ubiquitin protein ligase activity (already_in_goa_exact); E3-ligase ancestors = context_only/no_mapping.
- **Consistency:** Consistent, and the RING-less caveat is correctly recognized on BOTH sides. PN explicitly labels TRIM16 `TRIM / unclassified | ringless & SPRY`, and the review/notes repeatedly flag that TRIM16 LACKS a canonical RING and only autoubiquitinates atypically via its B-boxes (PMID:22629402). PN, review and notes agree the modern core function is galectin-3/ULK1-dependent lysophagy + aggrephagy scaffolding (PMID:27693506). No contradictions on biology.
- **PN story / NEW pressure:** Strong, defensible ADD pressure: the autophagy role is genuinely absent from GOA (confirmed — TRIM16 GOA has NO autophagy term). The review already adds it as two NEW annotations (GO:0006914 autophagy + GO:0030674 protein-macromolecule adaptor activity). PN's lysophagy node elevates the cargo-adaptor MF GO:0160247 (verified real) over bare protein binding — exactly the prescribed pattern, and more specific than the review's GO:0030674. **Important ontology correction:** the PN-node rationale claims "the current GO cache lacks a dedicated lysophagy process term" — this is WRONG: GO:0062093 lysophagy EXISTS (verified, def "selective autophagy in which a damaged lysosome is degraded by macroautophagy"). So a lysophagy **process** term is available in addition to the cargo-adaptor MF.
- **Mapping strategy:** CRITICAL — do NOT assign catalytic ligase MF on biological grounds, since TRIM16 is RING-less; but here the RING node's GO:0061630 is already_in_goa_exact via the EC/IEA + an EXP autoubiquitination annotation, so it is retained as an atypical-ligase capture rather than a PN-driven new catalytic claim. The PN correctly routes the headline biology through the cargo-adaptor MF (GO:0160247), not through catalytic ligase. Node is sound; only the "lysophagy term absent" rationale needs fixing, and the process term should be added.
- **Evidence alignment:** Strong overlap. PN cites the LIR/cargo-receptor review and the TRIM16-galectin-3 paper (= PMID:27693506, review HIGH) and 33791238 (TRIM review). Review refs (PMID:27693506, 22629402, 25127057) cover lysophagy + atypical ligase + LC3B.
- **Verdict:** Consistent; lysophagy ADD warranted; RING-less caveat correctly handled. **Recommended edits:** [MAP] fix the Lysophagy node rationale — GO:0062093 lysophagy exists; add it as the process target alongside GO:0160247. [YAML] upgrade the review's NEW GO:0030674 adaptor MF to the more specific GO:0160247 autophagy cargo adaptor activity, and add GO:0062093 lysophagy (involved_in, PMID:27693506) as the specific process. [YAML] retain GO:0061630 only as the atypical B-box autoubiquitination capture (do not assert RING catalytic ligase function).