## TRIM5
- **UniProt:** Q9C035 (TRIM5alpha) · **batch:** proteostasis-batch-2026-06-14 · **review status:** COMPLETE (very thorough, 40+ annotations)
- **PN placement:** 3 rows — `ALP|Autophagy substrate selection|Selective autophagy receptor|Xenophagy`; `UPS|E3 ubiquitin and UBL ligases|RING|TRIM / class IV|SPRY`; `UPS|Ubiquitin and UBL binding|E3 ligase|RING / TRIM class IV|SIM`. **PN-node mapping:** Xenophagy type → mapped/ok GO:0098792 xenophagy (more_specific_than_existing_goa); RING group → mapped/ok GO:0061630 ubiquitin protein ligase activity (already_in_goa_exact, x2); E3-ligase ancestors = context_only/too_broad or no_mapping.
- **Consistency:** Strongly consistent. PN (selective-autophagy receptor for virophagy + catalytic RING E3 ligase), review YAML, and notes all agree: TRIM5alpha is a genuine RING E3 (capsid-triggered K63 chains via UBE2V1-UBE2N → TAK1/NF-kB; PMID:21512573) AND a selective autophagy receptor that delivers HIV-1 capsid for degradation and scaffolds ULK1/BECN1 (PMID:25127057). Both the RING-ligase and the receptor arms are real and confirmed by the review's experimental annotations. TRIM5 has a functional RING (per the critical-distinction guideline) — catalytic ligase MF is correct here.
- **PN story / NEW pressure:** PN xenophagy is genuinely more specific than GOA: GOA carries GO:0006914 autophagy (IDA, PMID:25127057) but NOT GO:0098792 xenophagy (verified real). Virophagy of the HIV-1 capsid is textbook xenophagy → defensible ADD. The RING/ligase node is already_in_goa_exact (GO:0061630 present, IBA+IEA) — already captured. The selective-autophagy-**receptor MF** (cargo adaptor) is captured in the review as GO:0030674 protein-macromolecule adaptor activity (ACCEPT); the more specific GO:0160247 autophagy cargo adaptor activity (verified real, child of GO:0030674) would be a precise upgrade.
- **Mapping strategy:** No node change needed. Xenophagy projection is correct and appropriately narrower than GOA's bare autophagy. Pattern alert (selective-autophagy receptor → elevate cargo-adaptor MF): review uses GO:0030674; GO:0160247 is the more specific cargo-adaptor term and matches the receptor role.
- **Evidence alignment:** Strong overlap. PN cites the selective-autophagy/LIR review and the TRIM5-virophagy paper (= PMID:25127057, in review HIGH) plus 33791238/19489725/21490953 (TRIM/E3 reviews). Review's core refs (PMID:21512573, 25127057, 22291694, 18248090) cover both arms.
- **Verdict:** Consistent; xenophagy ADD warranted; RING ligase already captured. **Recommended edits:** [YAML] add GO:0098792 xenophagy (involved_in, supported by PMID:25127057) as a more-specific child of the existing autophagy annotation. [YAML] optionally upgrade the GO:0030674 adaptor MF to the more specific GO:0160247 autophagy cargo adaptor activity for the capsid-receptor role.
- **2026-06-18 follow-up:** Implemented both YAML edits: added GO:0098792 xenophagy and GO:0160247 autophagy cargo adaptor activity as NEW recommendations, and updated the core capsid-autophagy function to the more specific MF/process pair.