## TSG101
- **UniProt:** Q99816 · **batch:** proteostasis-pr-1217 · **review status:** COMPLETE
- **PN placement:** ALP row `Autophagy-Lysosome Pathway|Autophagosome closure maturation and lysosome fusion|Sealing of autophagophore membrane|ESCRT-I complex component`; UPS row `Ubiquitin Proteasome System|Ubiquitin and UBL binding|trafficking|ESCRT-I complex|UEV (Type 2)` ; **PN-node mapping:** ALP leaf mapped GO:0000813 ESCRT I complex (already_in_goa_exact); ALP "Sealing" group mapped GO:0000045 autophagosome assembly (more_specific_than_existing_goa); UPS subtype/type/group no_mapping; UPS class context_only GO:0140036 ubiquitin-modified protein reader activity
- **Consistency:** Strongly coherent. Notes, review, both PN rows, and node mappings agree: core = UEV-domain ESCRT-I adaptor (ubiquitin/PTAP binding), MVB cargo sorting, membrane fission, and ESCRT-I-dependent phagophore/autophagosome closure. Review ACCEPTs GO:0000813 ESCRT-I (multiple lines), retires ~30 generic protein-binding rows + transcription/cell-growth legacy terms, keeps viral budding/cytokinesis/exosome non-core. Negated GO:0061631 ubiquitin-conjugating-enzyme correctly ACCEPTed. No contradictions.
- **PN story / NEW pressure:** PN "Sealing of autophagophore membrane" group projects GO:0000045 autophagosome assembly as more_specific_than_existing_goa — and the review independently adds exactly this via **action: NEW** GO:0000045 (IMP, PMID:31519728 "core ESCRT-I components TSG101 and VPS28 ... required for autophagosome completion"; PMID:31010855). Review also MODIFYs the over-broad IEA GO:0016236 macroautophagy → GO:0000045. Conclusion: ADD — exact agreement between PN projection and review NEW term (OLS-verified GO:0000045).
- **Mapping strategy:** No change needed; this gene is the test case the projection got right. ESCRT-I leaf → GO:0000813 (exact, OLS-verified); Sealing group → GO:0000045 (correctly narrower than existing macroautophagy, avoiding the autophagosome-lysosome-fusion over-reach the rationale warns against). UPS-side no_mapping/context_only is appropriately conservative.
- **Evidence alignment:** PN ALP row title (ESCRT-I helical-assembly Nat Struct Mol Biol) is structural context; the review's load-bearing closure citations PMID:31519728 and PMID:31010855 are the autophagy-specific support and align with the PN projection's intent. No miscitation found.
- **Verdict:** Consistent; ACCEPT both mappings and endorse NEW GO:0000045. PN projection and review converge precisely (GO:0000813 + GO:0000045).
- **Recommended edits:** None required.