## UBA5
- **UniProt:** Q9GZZ9 · **batch:** proteostasis-batch-2026-06-07c · **review status:** complete (E1 core function + homodimerization; many protein-binding rows triaged)
- **PN placement:** `Translation|Cytosolic translation|Ribosome-associated QC|UFMylation`; `ALP|...|ERphagy|UFMylation of ER proteins`; `UPS|E1 activating enzymes|activation of UFM1` ; **PN-node mapping:** UFMylation type→GO:0071569; ERphagy→GO:0061709; E1 group→GO:0008641 (broad UBL E1 activity); RQC group→GO:0006515.
- **Consistency:** Strong. Notes/review/PN agree UBA5 is the UFM1 E1 (adenylation + Cys250 thioester, homodimer). Review ACCEPTs GO:0071566 (UFM1 activating enzyme activity, IDA x5), GO:0071569, ER membrane and cytosol; MODIFY on GO:0008641→GO:0071566. Reticulophagy KEEP_AS_NON_CORE present in GOA. No contradictions.
- **PN story / NEW pressure:** Everything the PN asserts (UFM1 activation, ufmylation, ERphagy) is already in GOA/review. GO:0006515 (verified) projected new_to_goa for the RQC group is broad; UBA5's RQC contribution is via ufmylation (GO:0071569) which is annotated. Conclusion: **already captured**; GO:0006515 over-reaches as a direct UBA5 annotation.
- **Mapping strategy:** Distinguish specific from broad MF — PN E1-group projects the broad GO:0008641 (ubiquitin-like modifier activating enzyme activity); the review correctly prefers the specific GO:0071566 (UFM1 activating enzyme activity) and even MODIFIES the IEA GO:0008641 toward it. PN should keep GO:0008641 as the safe group-level term but acknowledge the gene-level specific term is GO:0071566 (precedent: broader parents not propagated over specific). UBA5 does not change the node.
- **Evidence alignment:** PN row 2 cites the ER-phagy screen (PMID:32160526, in GOA as IDA for reticulophagy); review uses UniProt + cascade PMIDs (15071506 etc.). Overlap on the ufmylation/ERphagy evidence; PN E1 row is domain-signature only (IPR000594).
- **Verdict:** Consistent and complete; PN UFMylation/E1/ERphagy story fully captured. Specific GO:0071566 should be preferred over the PN's broad GO:0008641 at gene level; GO:0006515 not warranted as a direct add.