## VPS4A
- **UniProt:** Q9UN37 · **batch:** proteostasis-pr-1217 · **review status:** COMPLETE
- **PN placement:** 2 rows, ALP — (1) `Autophagosome closure maturation and lysosome fusion → Sealing of autophagophore membrane → ESCRT-III complex activity modulator`; (2) `Microautophagy → General microautophagy machinery → ESCRT-III complex component`. **PN-node mapping:** modulator leaf=context_only (GO:0000815, "should not project ESCRT-III membership to all members"); microautophagy-component leaf=mapped→GO:0000815; sealing group=mapped→GO:0000045; classes context_only (GO:0016236, GO:0016237). **Projected:** GO:0000045 (more_specific_than_existing_goa), GO:0000815 ESCRT III complex (more_specific_than_existing_goa).
- **Consistency:** CONTRADICTION on GO:0000815, and a labeling concern on goa_status. The review's central thesis (description + core_functions) is that VPS4A is the AAA ATPase that recognizes and *disassembles* ESCRT-III, NOT an ESCRT-III subunit; notes explicitly say "PN projection to GO:0000815 should not be added for VPS4A." The review instead asserts GO:1904903 ESCRT III complex disassembly (verified real via OLS) and GO:0016887 ATP hydrolysis. GOA contains no GO:0000815 or GO:0000045 — only GO:0016236 — so the projected goa_status "more_specific_than_existing_goa" is misleading (GO:0000815 would be a new, contested CC, not a refinement).
- **PN story / NEW pressure:** Mixed. PN's "ESCRT-III activity modulator" placement is biologically apt and matches the review's disassembly framing — but the microautophagy "ESCRT-III complex component" leaf miscategorizes VPS4A as a complex member. The accurate molecular story (ATP-driven ESCRT-III disassembly) is captured by the review's GO:1904903/GO:0016887; no new term needed.
- **Mapping strategy:** Change recommended. The microautophagy-component leaf→GO:0000815 should be context_only/no_mapping for VPS4A (as the sealing modulator leaf already correctly is), since VPS4A is not an ESCRT-III subunit. GO:0000815 over-reaches; this matches the rejected broader-projection precedent. GO:0000045 also unsupported here (review keeps autophagy rows MARK_AS_OVER_ANNOTATED / late-microautophagy UNDECIDED).
- **Evidence alignment:** Partial. PN cites the MDPI autophagosome-closure review and PMID-bearing "CHMP2A as a regulator of phagophore closure" (Nat Commun). Review is built on VPS4-specific primary/structural literature (PMID:17928862, 18606141, 36604498, 20616062) not in PN.
- **Verdict:** ESCRT-III-modulator framing consistent; GO:0000815 projection contradicts the review (VPS4A disassembles, not member). **Recommended edits:** none to YAML; reclassify PN microautophagy leaf→GO:0000815 to context_only for VPS4A and drop/relabel the GO:0000815 and GO:0000045 projections.