## VTI1A
- **UniProt:** Q96AJ9 · **batch:** proteostasis-pr-1217 · **review status:** COMPLETE
- **PN placement:** `ALP|Autophagosome closure maturation and lysosome fusion|Sealing of autophagophore membrane|ESCRT-III complex activity modulator` and `ALP|Microautophagy|General microautophagy machinery|ESCRT-III complex component` ; **PN-node mapping:** group `Sealing` = mapped/ok_for_propagation → GO:0000045 autophagosome assembly; type `ESCRT-III complex component` = mapped/ok_for_propagation → GO:0000815 ESCRT III complex; class nodes context_only.
- **Consistency:** Mostly consistent on the trafficking/SNARE core (UniProt, PMID:18195106, deep-research notes, and review all agree on endosome→TGN retrograde SNARE function and autophagic-flux involvement via PMID:24095276). One hard contradiction: the PN type-node projects **GO:0000815 ESCRT III complex** as ok_for_propagation, but the review and notes explicitly state VTI1A "is not an ESCRT-III complex component" — it is a SNARE intersecting ESCRT/CHMP2B autophagy phenotypes only via STX13/Vti1a trafficking.
- **PN story / NEW pressure:** PN frames VTI1A as an ESCRT-III modulator/component for phagophore sealing/microautophagy. Verified GO:0000815 (OLS) is a complex-membership CC term; asserting it for a SNARE over-reaches. The genuinely supported autophagy content (autophagy, macroautophagy, autophagosome) is already captured in existing annotations from PMID:24095276. **Conclusion: over-reaches** for ESCRT-III; autophagy role already captured.
- **Mapping strategy:** GO:0000815 should NOT propagate to VTI1A (recommend status→context_only for the microautophagy type-node, as the sibling "modulator" node already is). GO:0000045 autophagosome assembly (verified: phagophore-enclosure process) is narrower/different from the review's evidence, which supports flux/maturation (macroautophagy + autophagosome localization), not assembly — projection is a mismatch but at worst broader-context, lower priority than the ESCRT-III error.
- **Evidence alignment:** Strong overlap. PN ref "Syntaxin 13… required for autophagosome maturation" = review PMID:24095276 (autophagy/autophagosome rows). PN "Role of Vti1a… nervous system disorders" (Frontiers review) is not separately cited in the YAML; the YAML adds primary SNARE papers (PMID:18195106, 19138172, 23677696) the PN rows omit.
- **Verdict:** Trafficking core solid; **PN ESCRT-III complex projection (GO:0000815) contradicts the review and is biologically wrong for a SNARE** — demote to context_only/no propagation. Autophagy already captured; no new GO term warranted.