## ZFYVE26
- **UniProt:** Q68DK2 (spastizin/SPG15/FYVE-CENT) · **batch:** proteostasis-pr-1217 · **review status:** COMPLETE
- **PN placement:** `ALP|Autophagosome closure maturation and lysosome fusion|Class 3 PI3K complex 2, direct|Modulator of class 3 PI3K complex 2 activity` and `ALP|Autophagic lysosome reformation|Specific function… unknown` ; **PN-node mapping:** PI3K-complex nodes all context_only/too_broad → GO:0035032 (class III PI3K complex), explicitly "should not project CC membership"; ALR group = no_mapping (unknown); ALR class = context_only → GO:0007040 lysosome organization.
- **Consistency:** Strongly consistent. Deep-research notes, review YAML, PN notes, and PN mappings all converge: core = PI3P-binding FYVE protein in AP5-SPG11-SPG15 endolysosomal membrane remodeling / ALR / autophagosome maturation. The PN note's "Beclin1-UVRAG-Rubicon… required for autophagosome maturation" matches the review's NEW GO:0097352 row. No contradictions.
- **PN story / NEW pressure:** PN asserts (a) class III PI3K complex *modulation* — review correctly does NOT claim complex membership, using GO:0032266 PI3P binding + lysosome organization instead (matches PN's context_only decision); and (b) ALR with "specific function unknown." Review supplies the mechanism: GO:0007040, GO:0097212 lysosomal membrane organization, GO:0097753 membrane bending, plus NEW GO:0097352 autophagosome maturation (**OLS-verified real**). ALR itself has **no GO term** (OLS search empty) — review's `proposed_new_terms` "autophagic lysosome reformation" under GO:0007040 is well-justified. **Conclusion: NEW term (ALR) defensible; autophagosome maturation is a defensible ADD already in review; class-III-PI3K membership correctly NOT added.**
- **Mapping strategy:** No change needed to PI3K nodes (context_only is right; precedent-aligned — narrower review terms, no spurious CC propagation). ALR group could be upgraded from no_mapping toward GO:0007040 lysosome organization (the review's accepted core, and the class-node target), but PN's "unknown specific function" caution is reasonable; review is narrower than the PN class-level GO:0007040, never broader.
- **Evidence alignment:** Both PN refs are in the review. PN "Defective autophagy in spastizin… HSP type 15" = PMID:24030950 (autophagosome maturation); PN "spastizin and spatacsin mediate ALR" = PMID:25365221 (lysosome organization/ALR). Review adds PMID:40175557 (2025 structure), 23825025 (AP-5), 37871017, 20208530 the PN rows omit.
- **Verdict:** Fully consistent; **review enriches the PN "function unknown" ALR node with verified terms** (GO:0097352 add + ALR proposed-new-term). Mappings sound; no demotions needed.