{
  "method": "Biopython 1.85 global BLOSUM62, gap open -10, extension -0.5",
  "inputs": [
    {
      "path": "genes/human/AFAP1L2/AFAP1L2-uniprot.txt",
      "accession": "Q8N4X5",
      "length": 818,
      "sequence_sha256": "aa68e3b2f06b2397ca9a08f00b307b10e5b4fcb850b0e1fceb95826a5d26dd44"
    },
    {
      "path": "genes/HORSE/AFAP1L2/AFAP1L2-uniprot.txt",
      "accession": "A0A9L0RQI4",
      "length": 850,
      "sequence_sha256": "e860328ec80f1555eb2d8a271ff7dbd8c54832d366377e654074bd686b4525d5"
    }
  ],
  "score": 3683.5,
  "paired_residues": 818,
  "identical_residues": 725,
  "identity_among_paired": 0.8863080684596577,
  "coverage_left": 1.0,
  "coverage_right": 0.9623529411764706,
  "alignment": "target            0 ME-----------RYKALEQLLTELDDFLKILDQENLSSTALVKKSCLAELLRLYTKSSS\n                  0 |.-----------...|||||||||||||||||||||||||.||||.|||||||||||||\nquery             0 MRSTMWVLLANTATSNALEQLLTELDDFLKILDQENLSSTAEVKKSGLAELLRLYTKSSS\n\ntarget           49 SDEEYIYMNKVTINKQQNAESQGKAPEEQGLLPNGEPSQHSSAPQKSLPDLPPPKMIPER\n                 60 ||||||||||||..||||||||.|.||||..|.|||||||.||||||||||||||.||||\nquery            60 SDEEYIYMNKVTVHKQQNAESQDKVPEEQSPLTNGEPSQHTSAPQKSLPDLPPPKIIPER\n\ntarget          109 KQLAIPKTESPEGYYEEAEPYDTSLNEDGEAVSSSYESYDEEDGSKGKSAPYQWPSPEAG\n                120 |||.||..||||||||||||||||||||||||||||||||||...||||||||||||||.\nquery           120 KQLSIPRIESPEGYYEEAEPYDTSLNEDGEAVSSSYESYDEEESNKGKSAPYQWPSPEAS\n\ntarget          169 IELMRDARICAFLWRKKWLGQWAKQLCVIKDNRLLCYKSSKDHSPQLDVNLLGSSVIHKE\n                180 |||||||||||||||||||||||||||||||.||||||||||||||||||||||||.|||\nquery           180 IELMRDARICAFLWRKKWLGQWAKQLCVIKDTRLLCYKSSKDHSPQLDVNLLGSSVVHKE\n\ntarget          229 KQVRKKEHKLKITPMNADVIVLGLQSKDQAEQWLRVIQEVSGLPSEGASEGNQYTPDAQR\n                240 |||||||||||||||||||||||||||||||||||||||||||||||.||||||||||||\nquery           240 KQVRKKEHKLKITPMNADVIVLGLQSKDQAEQWLRVIQEVSGLPSEGVSEGNQYTPDAQR\n\ntarget          289 FNCQKPDIAEKYLSASEYGSSVDGHPEVPETKDVKKKCSAGLKLSNLMNLGRKKSTSLEP\n                300 .|||||||.||.|||||||||..|||||||||||||||||||||||||||||||||||||\nquery           300 PNCQKPDITEKFLSASEYGSSIEGHPEVPETKDVKKKCSAGLKLSNLMNLGRKKSTSLEP\n\ntarget          349 VERSLETSSYLNVLVNSQWKSRWCSVRDNHLHFYQDRNRSKVAQQPLSLVGCEVVPDPSP\n                360 .|||||||||||||||||||||||||||.|||||||||||||||||||||||||||||||\nquery           360 PERSLETSSYLNVLVNSQWKSRWCSVRDSHLHFYQDRNRSKVAQQPLSLVGCEVVPDPSP\n\ntarget          409 DHLYSFRILHKGEELAKLEAKSSEEMGHWLGLLLSESGSKTDPEEFTYDYVDADRVSCIV\n                420 ||||||||||.|||||||||||||||||||||||||||||||||||||||||||||||||\nquery           420 DHLYSFRILHNGEELAKLEAKSSEEMGHWLGLLLSESGSKTDPEEFTYDYVDADRVSCIV\n\ntarget          469 SAAKNSLLLMQRKFSEPNTYIDGLPSQDRQEELYDDVDLSELTAAVEPTEEATPVADDPN\n                480 |||||||||||||||||||||||||||||||.|||||..|||.|.|||||||||..|...\nquery           480 SAAKNSLLLMQRKFSEPNTYIDGLPSQDRQEMLYDDVEMSELSATVEPTEEATPATDALD\n\ntarget          529 ERESDRVYLDLTPVKSFLHGPSSAQAQASSPTLSCLDNATEALPADSGPGPTPDEPCIKC\n                540 |...|||||||||||||||...||||||.||.||.||...||||||.||.|||||.....\nquery           540 ESGPDRVYLDLTPVKSFLHSSGSAQAQAPSPPLSHLDPLAEALPADPGPSPTPDEALEMS\n\ntarget          589 PENLG-EQQLESLEPEDPSLRITTVKIQTEQQRISFPPSCPDAVVATPPGASPPVKDRLR\n                600 ||...-..|.|.||.|.||||.|.||||||||.||||.||||||..||.|||||||||||\nquery           600 PETPELQMQQENLESEEPSLRTTMVKIQTEQQKISFPSSCPDAVALTPAGASPPVKDRLR\n\ntarget          648 VTSA--------------------EIKLGKNRTEAEVKRYTEEKERLEKKKEEIRGHLAQ\n                660 ||.|--------------------||||||||||||||||||||||||.|||||||||||\nquery           660 VTTADEQTEVQRGEGTCPRSHSKEEIKLGKNRTEAEVKRYTEEKERLERKKEEIRGHLAQ\n\ntarget          688 LRKEKRELKETLLKCTDKEVLASLEQKLKEIDEECRGEESRRVDLELSIMEVKDNLKKAE\n                720 ||||||||||||.|||||.|||||||||||..||||.||||||||||.|.||||||||||\nquery           720 LRKEKRELKETLSKCTDKGVLASLEQKLKEMEEECRIEESRRVDLELNIVEVKDNLKKAE\n\ntarget          748 AGPVTLGTTVDTTHLENVSPRPKAVTPASAPDCTPVNSATTLKNRPLSVVVTGKGTVLQK\n                780 ||||||||||||||||.|||||||.||..|||||||||||.||||||||.||||||||||\nquery           780 AGPVTLGTTVDTTHLESVSPRPKAATPTPAPDCTPVNSATALKNRPLSVMVTGKGTVLQK\n\ntarget          808 AKEWEKKGAS 818\n                840 |||||||||| 850\nquery           840 AKEWEKKGAS 850\n",
  "limitations": "Pairwise similarity does not establish reciprocal orthology, full-length gene-model correctness or functional conservation."
}