---
pmid: '18757743'
title: Transcriptome-based systematic identification of extracellular matrix proteins.
authors:
- Manabe R
- Tsutsui K
- Yamada T
- Kimura M
- Nakano I
- Shimono C
- Sanzen N
- Furutani Y
- Fukuda T
- Oguri Y
- Shimamoto K
- Kiyozumi D
- Sato Y
- Sado Y
- Senoo H
- Yamashina S
- Fukuda S
- Kawai J
- Sugiura N
- Kimata K
- Hayashizaki Y
- Sekiguchi K
journal: Proc Natl Acad Sci U S A
year: '2008'
full_text_available: false
pmcid: PMC2529034
doi: 10.1073/pnas.0803640105
pubmed_publication_types:
- Journal Article
- Research Support, Non-U.S. Gov't
publication_type: PRIMARY_RESEARCH
---

# Transcriptome-based systematic identification of extracellular matrix proteins.
**Authors:** Manabe R, Tsutsui K, Yamada T, Kimura M, Nakano I, Shimono C, Sanzen N, Furutani Y, Fukuda T, Oguri Y, Shimamoto K, Kiyozumi D, Sato Y, Sado Y, Senoo H, Yamashina S, Fukuda S, Kawai J, Sugiura N, Kimata K, Hayashizaki Y, Sekiguchi K
**Journal:** Proc Natl Acad Sci U S A (2008)
**DOI:** [10.1073/pnas.0803640105](https://doi.org/10.1073/pnas.0803640105)
**PMC:** [PMC2529034](https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2529034/)

## Abstract

1. Proc Natl Acad Sci U S A. 2008 Sep 2;105(35):12849-54. doi: 
10.1073/pnas.0803640105. Epub 2008 Aug 29.

Transcriptome-based systematic identification of extracellular matrix proteins.

Manabe R(1), Tsutsui K, Yamada T, Kimura M, Nakano I, Shimono C, Sanzen N, 
Furutani Y, Fukuda T, Oguri Y, Shimamoto K, Kiyozumi D, Sato Y, Sado Y, Senoo H, 
Yamashina S, Fukuda S, Kawai J, Sugiura N, Kimata K, Hayashizaki Y, Sekiguchi K.

Author information:
(1)Sekiguchi Biomatrix Signaling Project, Exploratory Research for Advanced 
Technology, Japan Science and Technology Agency, c/o Aichi Medical University, 
Nagakute, Aichi 480-1195, Japan.

Extracellular matrix (ECM), which provides critical scaffolds for all adhesive 
cells, regulates proliferation, differentiation, and apoptosis. Different cell 
types employ customized ECMs, which are thought to play important roles in the 
generation of so-called niches that contribute to cell-specific functions. The 
molecular entities of these customized ECMs, however, have not been elucidated. 
Here, we describe a strategy for transcriptome-wide identification of ECM 
proteins based on computational screening of >60,000 full-length mouse cDNAs for 
secreted proteins, followed by in vitro functional assays. These assays screened 
the candidate proteins for ECM-assembling activities, interactions with other 
ECM molecules, modifications with glycosaminoglycans, and cell-adhesive 
activities, and were then complemented with immunohistochemical analysis. We 
identified 16 ECM proteins, of which seven were localized in basement membrane 
(BM) zones. The identification of these previously unknown BM proteins allowed 
us to construct a body map of BM proteins, which represents the comprehensive 
immunohistochemistry-based expression profiles of the tissue-specific 
customization of BMs.

DOI: 10.1073/pnas.0803640105
PMCID: PMC2529034
PMID: 18757743 [Indexed for MEDLINE]

Conflict of interest statement: The authors declare no conflict of interest.