---
reference_id: PMID:22898498
title: "Two novel RING-type ubiquitin ligases, RGLG3 and RGLG4, are essential for jasmonate-mediated responses in Arabidopsis."
authors:
- Zhang X
- Wu Q
- Ren J
- Qian W
- He S
- Huang K
- Yu X
- Gao Y
- Huang P
- An C
journal: Plant Physiol
year: '2012'
doi: 10.1104/pp.112.203422
keywords:
- Acetates/pharmacology
- Amino Acids/metabolism
- "Arabidopsis/drug effects, enzymology, genetics, microbiology"
- "Arabidopsis Proteins/genetics, metabolism"
- Bacterial Proteins/metabolism
- Cyclopentanes/pharmacology
- Enzyme Activation
- "Feedback, Physiological"
- "Gene Expression Regulation, Enzymologic"
- "Gene Expression Regulation, Plant"
- "Genes, Plant"
- Indenes/metabolism
- Oxylipins/pharmacology
- Phylogeny
- Plant Diseases/microbiology
- Plant Immunity
- "Plant Roots/drug effects, enzymology, growth & development"
- "Plants, Genetically Modified/enzymology, genetics, growth & development"
- Pseudomonas syringae/pathogenicity
- "Recombinant Proteins/genetics, metabolism"
- Signal Transduction
- "Ubiquitin-Protein Ligases/genetics, metabolism"
- Ubiquitination
content_type: abstract_only
full_text_attempted: true
---

# Two novel RING-type ubiquitin ligases, RGLG3 and RGLG4, are essential for jasmonate-mediated responses in Arabidopsis.
**Authors:** Zhang X, Wu Q, Ren J, Qian W, He S, Huang K, Yu X, Gao Y, Huang P, An C
**Journal:** Plant Physiol (2012)
**DOI:** [10.1104/pp.112.203422](https://doi.org/10.1104/pp.112.203422)

## Content

1. Plant Physiol. 2012 Oct;160(2):808-22. doi: 10.1104/pp.112.203422. Epub 2012
Aug  16.

Two novel RING-type ubiquitin ligases, RGLG3 and RGLG4, are essential for 
jasmonate-mediated responses in Arabidopsis.

Zhang X(1), Wu Q, Ren J, Qian W, He S, Huang K, Yu X, Gao Y, Huang P, An C.

Author information:
(1)State Key Laboratory of Protein and Plant Gene Research, College of Life 
Sciences, Peking University, Beijing 100871, People's Republic of China.

Jasmonates (JAs) regulate various stress responses and development processes in 
plants, and the JA pathway is tightly controlled. In this study, we report the 
functional characterization of two novel RING-type ubiquitin ligases, RING 
DOMAIN LIGASE3 (RGLG3) and RGLG4, in modulating JA signaling. Both RGLG3 and 
RGLG4 possessed ubiquitin ligase activities and were widely distributed in 
Arabidopsis (Arabidopsis thaliana) tissues. Altered expression of RGLG3 and 
RGLG4 affected methyl JA-inhibited root growth and JA-inductive gene expression, 
which could be suppressed by the coronatine insensitive1 (coi1) mutant. rglg3 
rglg4 also attenuated the inhibitory effect of JA-isoleucine-mimicking 
coronatine on root elongation, and consistently, rglg3 rglg4 was resistant to 
the coronatine-secreting pathogen Pseudomonas syringae pv tomato DC3000, 
suggesting that RGLG3 and RGLG4 acted in response to the coronatine and promoted 
JA-mediated pathogen susceptibility. In addition, rglg3 rglg4 repressed 
wound-stunted plant growth, wound-stimulated expression of JA-responsive genes, 
and wound-induced JA biosynthesis, indicating their roles in JA-dependent wound 
response. Furthermore, both RGLG3 and RGLG4 responded to methyl JA, P. syringae 
pv tomato DC3000, and wounding in a COI1-dependent manner. Taken together, these 
results indicate that the ubiquitin ligases RGLG3 and RGLG4 are essential 
upstream modulators of JA signaling in response to various stimuli.

DOI: 10.1104/pp.112.203422
PMCID: PMC3461557
PMID: 22898498 [Indexed for MEDLINE]