---
pmid: '36931562'
title: Wheat derived glucuronokinase as a potential target for regulating ascorbic
  acid and phytic acid content with increased root length under drought and ABA stresses
  in Arabidopsis thaliana.
authors:
- Thakur N
- Flowerika
- Chaturvedi S
- Tiwari S
journal: Plant Sci
year: '2023'
full_text_available: false
doi: 10.1016/j.plantsci.2023.111671
pubmed_publication_types:
- Journal Article
publication_type: PRIMARY_RESEARCH
---

# Wheat derived glucuronokinase as a potential target for regulating ascorbic acid and phytic acid content with increased root length under drought and ABA stresses in Arabidopsis thaliana.
**Authors:** Thakur N, Flowerika, Chaturvedi S, Tiwari S
**Journal:** Plant Sci (2023)
**DOI:** [10.1016/j.plantsci.2023.111671](https://doi.org/10.1016/j.plantsci.2023.111671)

## Abstract

1. Plant Sci. 2023 Jun;331:111671. doi: 10.1016/j.plantsci.2023.111671. Epub 2023
 Mar 15.

Wheat derived glucuronokinase as a potential target for regulating ascorbic acid
and phytic acid content with increased root length under drought and ABA
stresses in Arabidopsis thaliana.

Thakur N(1), Flowerika(2), Chaturvedi S(1), Tiwari S(3).

Author information:
(1)Plant Tissue Culture and Genetic Engineering Lab, National Agri-Food
Biotechnology Institute (NABI), Department of Biotechnology, Ministry of Science
and Technology (Government of India), Sector-81, Mohali 140306, Punjab, India;
Department of Biotechnology, Panjab University, Chandigarh 160014, India.
(2)Plant Tissue Culture and Genetic Engineering Lab, National Agri-Food
Biotechnology Institute (NABI), Department of Biotechnology, Ministry of Science
and Technology (Government of India), Sector-81, Mohali 140306, Punjab, India;
Department of Biotechnology, Panjab University, Chandigarh 160014, India;
Department of Plant and Microbial Biology, University of Minnesota, Saint Paul,
MN 55108, USA.
(3)Plant Tissue Culture and Genetic Engineering Lab, National Agri-Food
Biotechnology Institute (NABI), Department of Biotechnology, Ministry of Science
and Technology (Government of India), Sector-81, Mohali 140306, Punjab, India.
Electronic address: siddharthdna@gmail.com.

Glucuronokinase (GlcAK) converts glucuronic acid into glucuronic
acid-1-phosphate, which is then converted into UDP-glucuronic acid (UDP-GlcA)
via myo-inositol oxygenase (MIOX) pathway. UDP-GlcA acts as a precursor in the
synthesis of nucleotide-sugar moieties forming cell wall biomass. GlcAK being
present at the bifurcation point between UDP-GlcA and ascorbic acid (AsA)
biosyntheses, makes it necessary to study its role in plants. In this study, the
three homoeologs of GlcAK gene from hexaploid wheat were overexpressed in
Arabidopsis thaliana. The GlcAK overexpressing transgenic lines showed decreased
contents of AsA and phytic acid (PA) as compared to control plants. Root length
and seed germination analyses under abiotic stress (drought and abscisic acid)
conditions revealed enhanced root length in transgenic lines as compared to
control plants. These results indicate that the MIOX pathway might be
contributing towards AsA biosynthesis as evident by the decreased AsA content in
the GlcAK overexpressing transgenic Arabidopsis thaliana plants. Findings of the
present study will enhance the understanding of the involvement of GlcAK gene in
MIOX pathway and subsequent physiological effects in plants.

Copyright © 2023 Elsevier B.V. All rights reserved.

DOI: 10.1016/j.plantsci.2023.111671
PMID: 36931562 [Indexed for MEDLINE]

Conflict of interest statement: Declaration of Competing Interest The authors
declare that they have no known competing financial interests or personal
relationships that could have appeared to influence the work reported in this
paper.