---
pmid: '8371782'
title: BUD2 encodes a GTPase-activating protein for Bud1/Rsr1 necessary for proper
  bud-site selection in yeast.
authors:
- Park HO
- Chant J
- Herskowitz I
journal: Nature
year: '1993'
full_text_available: false
doi: 10.1038/365269a0
pubmed_publication_types:
- Journal Article
- Research Support, Non-U.S. Gov't
publication_type: PRIMARY_RESEARCH
---

# BUD2 encodes a GTPase-activating protein for Bud1/Rsr1 necessary for proper bud-site selection in yeast.
**Authors:** Park HO, Chant J, Herskowitz I
**Journal:** Nature (1993)
**DOI:** [10.1038/365269a0](https://doi.org/10.1038/365269a0)

## Abstract

1. Nature. 1993 Sep 16;365(6443):269-74. doi: 10.1038/365269a0.

BUD2 encodes a GTPase-activating protein for Bud1/Rsr1 necessary for proper
bud-site selection in yeast.

Park HO(1), Chant J, Herskowitz I.

Author information:
(1)Department of Biochemistry & Biophysics, University of California, San
Francisco 94143-0448.

Cells of the budding yeast Saccharomyces cerevisiae bud at either axial or
bipolar sites depending on their cell type. Bud-site selection directs both cell
polarity and the cytoskeletal orientation during budding and is determined by at
least five genes: BUD1/RSR1, BUD2, BUD3, BUD4 and BUD5. Mutants defective in
BUD1, BUD2 or BUD5 choose bud sites randomly. Bud1 protein (Bud1p) has sequence
similarity to Ras, a small GTP-binding protein, and Bud5p is similar to Cdc25p
(refs 4, 5), a GDP-GTP exchange factor. Here we report that Bud2p is a
GTPase-activating protein (GAP) for Bud1p with a sequence similar to the
catalytic domain of rasGAPs, and that Bud2p purified from yeast stimulates GTP
hydrolysis by Bud1p. Chromosomal deletion of BUD2 causes a random budding
pattern but no obvious growth defect. Overexpression of BUD2 also causes a
random budding pattern by wild-type cells.

DOI: 10.1038/365269a0
PMID: 8371782 [Indexed for MEDLINE]