ARBA00043370 export across plasma membrane (GO:0140115)

id: ARBA00043370
description: >-
  Assigns GO:0140115 (export across plasma membrane) through 14 alternative condition sets that identify
  efflux transporters: bacterial SMR and Emr multidrug exporters, fungal MFS and ABC drug exporters, plant
  ABCB auxin transporters, metazoan ABCG2, sodium-exporting P-type ATPases, Na+/K+-ATPase alpha subunits,
  fungal Na+/H+ antiporters and Na+/Ca2+ exchangers.
status: COMPLETE
rule_type: ARBA
rule:
  rule_id: ARBA00043370
  condition_sets:
  - number: 1
    conditions:
    - condition_type: INTERPRO
      value: IPR001958
      curie: InterPro:IPR001958
      label: Tetracycline resistance protein TetA/multidrug resistance protein MdtG-like
      interpro_type: FAMILY
      negated: false
    - condition_type: INTERPRO
      value: IPR011701
      curie: InterPro:IPR011701
      label: Major facilitator superfamily
      interpro_type: FAMILY
      negated: false
    - condition_type: PANTHER
      value: PTHR23502
      curie: PANTHER:PTHR23502
      label: MAJOR FACILITATOR SUPERFAMILY
      negated: false
    notes: >-
      Swiss-Prot entries matching the whole conjunction: 7 (UniProtKB query, 2026-11-10); unreviewed entries:
      3002. Swiss-Prot matches: P28246 (bcr), Q5ABU7 (MDR1), P38124 (FLR1), Q6FRT6 (FLR1), Q6FRT5 (FLR2),
      P28873 (MDR1), P32071 (CYHR). Experimental annotation to GO:0140115 export across plasma membrane
      or an is_a/part_of descendant (QuickGO): P28246 bcr GO:0033228 L-cysteine export across plasma membrane
      IMP PMID:16820466 (EcoCyc); P38124 FLR1 GO:1990961 xenobiotic detoxification by transmembrane export
      across the plasma membrane IGI PMID:10572257 (SGD); Q5ABU7 MDR1 GO:1990961 xenobiotic detoxification
      by transmembrane export across the plasma membrane IDA PMID:11568478 (CGD); Q6FRT5 FLR2 GO:1990961
      xenobiotic detoxification by transmembrane export across the plasma membrane IMP PMID:28066366 (CGD);
      Q6FRT6 FLR1 GO:1990961 xenobiotic detoxification by transmembrane export across the plasma membrane
      IMP PMID:28066366 (CGD).
  - number: 2
    conditions:
    - condition_type: FUNFAM
      value: 1.20.1560.10:FF:000009
      curie: CATH.FunFam:1.20.1560.10:FF:000009
      label: ABC transporter B family member 1
      negated: false
    - condition_type: TAXON
      value: Viridiplantae
      curie: NCBITaxon:33090
      label: Viridiplantae
      negated: false
    notes: >-
      Swiss-Prot entries matching the whole conjunction: 11 (UniProtKB query, 2026-11-10); unreviewed
      entries: 4767. Swiss-Prot matches: O80725 (ABCB4), Q9LJX0 (ABCB19), Q9M1Q9 (ABCB21), Q9C7F8 (ABCB13),
      Q9FWX7 (ABCB11), Q9ZR72 (ABCB1), Q9FWX8 (ABCB12), Q9M0M2 (ABCB9), and 3 more. Experimental annotation
      to GO:0140115 export across plasma membrane or an is_a/part_of descendant (QuickGO): O80725 ABCB4
      GO:0010315 auxin export across the plasma membrane IDA PMID:23147222 (TAIR); Q9FWX7 ABCB11 GO:0010315
      auxin export across the plasma membrane IMP PMID:35528937 (UniProt); Q9LJX0 ABCB19 GO:0010315 auxin
      export across the plasma membrane IDA PMID:38513023 (UniProt); Q9M1Q9 ABCB21 GO:0010315 auxin export
      across the plasma membrane IDA PMID:23147222 (TAIR); Q9ZR72 ABCB1 GO:0010315 auxin export across
      the plasma membrane IDA PMID:23147222 (TAIR).
  - number: 3
    conditions:
    - condition_type: FUNFAM
      value: 1.10.3730.20:FF:000001
      curie: CATH.FunFam:1.10.3730.20:FF:000001
      label: Quaternary ammonium compound resistance transporter SugE
      negated: false
    - condition_type: TAXON
      value: Escherichia
      curie: NCBITaxon:561
      label: Escherichia
      negated: false
    notes: >-
      Swiss-Prot entries matching the whole conjunction: 47 (UniProtKB query, 2026-11-10); unreviewed
      entries: 56. Swiss-Prot matches: P69210 (mdtI), P69212 (mdtJ), P23895 (emrE), P69937 (gdx), P69211
      (mdtI), P69213 (mdtJ), A1ABE4 (mdtI), A7ZM58 (mdtI), and 39 more. Experimental annotation to GO:0140115
      export across plasma membrane or an is_a/part_of descendant (QuickGO): P23895 emrE GO:1990961 xenobiotic
      detoxification by transmembrane export across the plasma membrane IDA PMID:24448799 (ComplexPortal);
      P69210 mdtI GO:1990961 xenobiotic detoxification by transmembrane export across the plasma membrane
      IMP PMID:11566977 (EcoCyc); P69212 mdtJ GO:1990961 xenobiotic detoxification by transmembrane export
      across the plasma membrane IMP PMID:11566977 (EcoCyc); P69937 gdx GO:1990961 xenobiotic detoxification
      by transmembrane export across the plasma membrane IMP PMID:11948170 (EcoCyc).
  - number: 4
    conditions:
    - condition_type: FUNFAM
      value: 1.20.1420.30:FF:000001
      curie: CATH.FunFam:1.20.1420.30:FF:000001
      label: sodium/calcium exchanger 1 isoform X1
      negated: false
    - condition_type: FUNFAM
      value: 1.20.1420.30:FF:000003
      curie: CATH.FunFam:1.20.1420.30:FF:000003
      label: sodium/calcium exchanger 1 isoform X1
      negated: false
    - condition_type: TAXON
      value: Rattus
      curie: NCBITaxon:10114
      label: Rattus
      negated: false
    notes: >-
      Swiss-Prot entries matching the whole conjunction: 3 (UniProtKB query, 2026-11-10); unreviewed entries:
      9. Swiss-Prot matches: Q01728 (Slc8a1), P48768 (Slc8a2), P70549 (Slc8a3). Experimental annotation
      to GO:0140115 export across plasma membrane or an is_a/part_of descendant (QuickGO): P48768 Slc8a2
      GO:1990034 calcium ion export across plasma membrane IDA PMID:17343909 (RGD); P70549 Slc8a3 GO:1990034
      calcium ion export across plasma membrane IDA PMID:17343909 (RGD); Q01728 Slc8a1 GO:0036376 sodium
      ion export across plasma membrane IDA PMID:11916852 (BHF-UCL).
  - number: 5
    conditions:
    - condition_type: FUNFAM
      value: 3.40.50.300:FF:000054
      curie: CATH.FunFam:3.40.50.300:FF:000054
      label: ABC multidrug transporter atrF
      negated: false
    - condition_type: TAXON
      value: Pichiomycetes
      curie: NCBITaxon:3239874
      label: Pichiomycetes
      negated: false
    notes: >-
      Swiss-Prot entries matching the whole conjunction: 6 (UniProtKB query, 2026-11-10); unreviewed entries:
      359. Swiss-Prot matches: A0A2H1A768 (CDR1), Q5ANA3 (CDR1), P78595 (CDR2), P43071 (CDR1), O74676
      (CDR4), O42690 (CDR3). Experimental annotation to GO:0140115 export across plasma membrane or an
      is_a/part_of descendant (QuickGO): A0A2H1A768 CDR1 GO:1990961 xenobiotic detoxification by transmembrane
      export across the plasma membrane IMP PMID:36836283 (CGD); P78595 CDR2 GO:1990961 xenobiotic detoxification
      by transmembrane export across the plasma membrane IMP PMID:9756759 (CGD); Q5ANA3 CDR1 GO:1990961
      xenobiotic detoxification by transmembrane export across the plasma membrane IDA PMID:11709310 (CGD).
  - number: 6
    conditions:
    - condition_type: FUNFAM
      value: 3.40.50.300:FF:000622
      curie: CATH.FunFam:3.40.50.300:FF:000622
      label: ATP-binding cassette sub-family G member 2
      negated: false
    - condition_type: TAXON
      value: Metazoa
      curie: NCBITaxon:33208
      label: Metazoa
      negated: false
    notes: >-
      Swiss-Prot entries matching the whole conjunction: 7 (UniProtKB query, 2026-11-10); unreviewed entries:
      2690. Swiss-Prot matches: Q9UNQ0 (ABCG2), Q99P81 (Abcg3), Q4GZT4 (ABCG2), Q7TMS5 (Abcg2), Q80W57
      (Abcg2), Q8MIB3 (ABCG2), Q5MB13 (ABCG2). Experimental annotation to GO:0140115 export across plasma
      membrane or an is_a/part_of descendant (QuickGO): Q7TMS5 Abcg2 GO:0140115 export across plasma membrane
      IDA PMID:20538997 (MGI); Q80W57 Abcg2 GO:0140115 export across plasma membrane IDA PMID:15773906
      (ARUK-UCL); Q9UNQ0 ABCG2 GO:1990961 xenobiotic detoxification by transmembrane export across the
      plasma membrane IMP PMID:18056989 (UniProt).
  - number: 7
    conditions:
    - condition_type: FUNFAM
      value: 1.20.1110.10:FF:000015
      curie: CATH.FunFam:1.20.1110.10:FF:000015
      label: Sodium ion P-type ATPase
      negated: false
    - condition_type: FUNFAM
      value: 3.40.1110.10:FF:000039
      curie: CATH.FunFam:3.40.1110.10:FF:000039
      label: Sodium P-type ATPase
      negated: false
    - condition_type: TAXON
      value: Debaryomycetaceae
      curie: NCBITaxon:766764
      label: Debaryomycetaceae
      negated: false
    notes: >-
      Swiss-Prot entries matching the whole conjunction: 2 (UniProtKB query, 2026-11-10); unreviewed entries:
      62. Swiss-Prot matches: O13398 (ENA2), O13397 (ENA1). Experimental annotation to GO:0140115 export
      across plasma membrane or an is_a/part_of descendant (QuickGO): O13397 ENA1 GO:0036376 sodium ion
      export across plasma membrane IMP PMID:9430707 (UniProt); O13398 ENA2 GO:0097623 potassium ion export
      across plasma membrane IMP PMID:9430707 (UniProt).
  - number: 8
    conditions:
    - condition_type: FUNFAM
      value: 1.20.1110.10:FF:000038
      curie: CATH.FunFam:1.20.1110.10:FF:000038
      label: Sodium/potassium-transporting ATPase subunit alpha
      negated: false
    - condition_type: FUNFAM
      value: 1.20.1110.10:FF:000069
      curie: CATH.FunFam:1.20.1110.10:FF:000069
      label: Sodium/potassium-transporting ATPase subunit alpha
      negated: false
    - condition_type: TAXON
      value: Primates
      curie: NCBITaxon:9443
      label: Primates
      negated: false
    notes: >-
      Swiss-Prot entries matching the whole conjunction: 3 (UniProtKB query, 2026-11-10); unreviewed entries:
      190. Swiss-Prot matches: P05023 (ATP1A1), P13637 (ATP1A3), Q5RDR3 (ATP1A1). Experimental annotation
      to GO:0140115 export across plasma membrane or an is_a/part_of descendant (QuickGO): P05023 ATP1A1
      GO:0036376 sodium ion export across plasma membrane IDA PMID:10636900 (BHF-UCL); P13637 ATP1A3 GO:0036376
      sodium ion export across plasma membrane IDA PMID:10636900 (BHF-UCL).
  - number: 9
    conditions:
    - condition_type: FUNFAM
      value: 1.20.1250.20:FF:000019
      curie: CATH.FunFam:1.20.1250.20:FF:000019
      label: Multidrug resistance protein B
      negated: false
    - condition_type: FUNFAM
      value: 1.20.1720.10:FF:000002
      curie: CATH.FunFam:1.20.1720.10:FF:000002
      label: Multidrug resistance protein B
      negated: false
    - condition_type: TAXON
      value: Bacteria
      curie: NCBITaxon:2
      label: Bacteria
      negated: false
    notes: >-
      Swiss-Prot entries matching the whole conjunction: 4 (UniProtKB query, 2026-11-10); unreviewed entries:
      876. Swiss-Prot matches: P0AEJ0 (emrB), P52600 (emrY), P0AEJ1 (emrB), P44927 (emrB). Experimental
      annotation to GO:0140115 export across plasma membrane or an is_a/part_of descendant (QuickGO):
      P0AEJ0 emrB GO:1990961 xenobiotic detoxification by transmembrane export across the plasma membrane
      IMP PMID:1409590 (EcoCyc); P52600 emrY GO:1990961 xenobiotic detoxification by transmembrane export
      across the plasma membrane IDA PMID:12501312 (EcoCyc).
  - number: 10
    conditions:
    - condition_type: FUNFAM
      value: 1.20.1560.10:FF:000021
      curie: CATH.FunFam:1.20.1560.10:FF:000021
      label: ABC transporter B family member 6
      negated: false
    - condition_type: FUNFAM
      value: 1.20.1560.10:FF:000028
      curie: CATH.FunFam:1.20.1560.10:FF:000028
      label: ABC transporter B family member 20
      negated: false
    - condition_type: FUNFAM
      value: 1.20.1560.10:FF:000049
      curie: CATH.FunFam:1.20.1560.10:FF:000049
      label: ABC transporter B family member 6
      negated: false
    notes: >-
      Swiss-Prot entries matching the whole conjunction: 2 (UniProtKB query, 2026-11-10); unreviewed entries:
      609. Swiss-Prot matches: Q9M3B9 (ABCB20), Q8LPT1 (ABCB6). Experimental annotation to GO:0140115
      export across plasma membrane or an is_a/part_of descendant (QuickGO): Q8LPT1 ABCB6 GO:0010315 auxin
      export across the plasma membrane IMP PMID:35528937 (UniProt); Q9M3B9 ABCB20 GO:0010315 auxin export
      across the plasma membrane IMP PMID:35528937 (UniProt).
  - number: 11
    conditions:
    - condition_type: FUNFAM
      value: 2.70.150.10:FF:000016
      curie: CATH.FunFam:2.70.150.10:FF:000016
      label: Calcium-transporting P-type ATPase putative
      negated: false
    - condition_type: FUNFAM
      value: 3.40.50.1000:FF:000028
      curie: CATH.FunFam:3.40.50.1000:FF:000028
      label: Calcium-transporting P-type ATPase, putative
      negated: false
    - condition_type: TAXON
      value: Embryophyta
      curie: NCBITaxon:3193
      label: Embryophyta
      negated: false
    notes: >-
      Swiss-Prot entries matching the whole conjunction: 2 (UniProtKB query, 2026-11-10); unreviewed entries:
      14. Swiss-Prot matches: Q7XB51 (ENA1), C1L360 (ENA1). Experimental annotation to GO:0140115 export
      across plasma membrane or an is_a/part_of descendant (QuickGO): C1L360 ENA1 GO:0097623 potassium
      ion export across plasma membrane IMP PMID:19757095 (UniProt); Q7XB51 ENA1 GO:0036376 sodium ion
      export across plasma membrane IDA PMID:14617094 (UniProt).
  - number: 12
    conditions:
    - condition_type: FUNFAM
      value: 1.20.1250.20:FF:000172
      curie: CATH.FunFam:1.20.1250.20:FF:000172
      label: MFS multidrug resistance transporter
      negated: false
    - condition_type: TAXON
      value: Pezizomycotina
      curie: NCBITaxon:147538
      label: Pezizomycotina
      negated: false
    notes: >-
      Swiss-Prot entries matching the whole conjunction: 8 (UniProtKB query, 2026-11-10); unreviewed entries:
      3563. Swiss-Prot matches: G3Y4N5 (cexA), A0A254TZW7 (cexA), A0A345BJN8 (clz9), B8MKZ1 (tstD), A0A345BJP9
      (clz19), A0A3G1DIQ9 (R5), B8MKZ7 (tstL), A0A3G1DIJ8 (M6). Experimental annotation to GO:0140115
      export across plasma membrane or an is_a/part_of descendant (QuickGO): A0A254TZW7 cexA GO:0140115
      export across plasma membrane IDA PMID:31062025 (UniProt); G3Y4N5 cexA GO:0140115 export across
      plasma membrane IGI PMID:32990722 (UniProt).
  - number: 13
    conditions:
    - condition_type: FUNFAM
      value: 1.20.1530.20:FF:000015
      curie: CATH.FunFam:1.20.1530.20:FF:000015
      label: Na(+)/H(+) antiporter 2
      negated: false
    - condition_type: TAXON
      value: Fungi
      curie: NCBITaxon:4751
      label: Fungi
      negated: false
    notes: >-
      Swiss-Prot entries matching the whole conjunction: 4 (UniProtKB query, 2026-11-10); unreviewed entries:
      1780. Swiss-Prot matches: Q99271 (NHA1), O14123 (sod22), Q99173 (SOD2), O42701 (SOD22). Experimental
      annotation to GO:0140115 export across plasma membrane or an is_a/part_of descendant (QuickGO):
      Q99271 NHA1 GO:0036376 sodium ion export across plasma membrane IMP PMID:9802016 (SGD).
  - number: 14
    conditions:
    - condition_type: FUNFAM
      value: 2.40.30.170:FF:000003
      curie: CATH.FunFam:2.40.30.170:FF:000003
      label: Multidrug resistance protein A
      negated: false
    - condition_type: TAXON
      value: Gammaproteobacteria
      curie: NCBITaxon:1236
      label: Gammaproteobacteria
      negated: false
    notes: >-
      Swiss-Prot entries matching the whole conjunction: 3 (UniProtKB query, 2026-11-10); unreviewed entries:
      1578. Swiss-Prot matches: P27303 (emrA), P52599 (emrK), P44928 (emrA). Experimental annotation to
      GO:0140115 export across plasma membrane or an is_a/part_of descendant (QuickGO): P27303 emrA GO:1990961
      xenobiotic detoxification by transmembrane export across the plasma membrane IMP PMID:1409590 (EcoCyc);
      P52599 emrK GO:1990961 xenobiotic detoxification by transmembrane export across the plasma membrane
      IDA PMID:12501312 (EcoCyc).
  go_annotations:
  - go_id: GO:0140115
    go_label: export across plasma membrane
    aspect: BP
  entries: []
  reviewed_protein_count: 0
  unreviewed_protein_count: 0
  created_date: '2023-10-01'
  modified_date: '2026-07-18'
  source_condition_set_count: 14
  retrieved_date: '2026-11-09'
review_summary: >-
  All 14 live condition sets were examined by retrieving the Swiss-Prot entries that match the whole conjunction
  and looking up their curated annotations. Every set has at least one Swiss-Prot match with an experimental
  annotation to GO:0140115 or an is_a/part_of descendant: xenobiotic detoxification by transmembrane export
  across the plasma membrane (GO:1990961), sodium, potassium or calcium ion export across plasma membrane
  (GO:0036376, GO:0097623, GO:1990034), auxin export across the plasma membrane (GO:0010315) and L-cysteine
  export across plasma membrane (GO:0033228). In several sets only some of the reviewed matches are annotated;
  for example 4 of 47 in set 3 and 2 of 8 in set 12. The check uses Swiss-Prot, the source ARBA rules
  are mined from, and does not measure precision on unreviewed entries. The curator concern in geneontology/go-annotation
  issue 6140 could not be read during this review, so the set or protein it refers to is not identified
  here.
action: ACCEPT
action_rationale: >-
  Each condition set is a narrow conjunction, usually with a taxon condition, and each has reviewed members
  with experimental evidence of export across the plasma membrane. The parent term is true of all of them
  and is less specific than the substrate-specific terms the reviewed members carry. Confidence is moderate
  because the unannotated reviewed matches were not checked individually and the cited curator issue was
  not available.
suggested_modifications:
- >-
  No change to the rule is proposed. If the matter raised in geneontology/go-annotation issue 6140 concerns
  a specific protein, identify which of the 14 sets matches it and assess that set alone.
annotation_assessments:
- annotation_kind: GO_TERM
  value: GO:0140115
  label: export across plasma membrane
  assessment: CORRECT
  correct_examples:
  - P23895
  - P69210
  - Q9UNQ0
  - Q7TMS5
  - P05023
  - Q01728
  - Q99271
  - P27303
  - Q9LJX0
  - A0A254TZW7
  notes: >-
    P23895 emrE GO:1990961 IDA PMID:24448799; P69210 mdtI GO:1990961 IMP PMID:11566977; Q7TMS5 Abcg2 GO:0140115
    IDA PMID:20538997; Q9UNQ0 ABCG2 GO:1990961 IMP PMID:18056989; P05023 ATP1A1 GO:0036376 IDA PMID:10636900;
    Q01728 Slc8a1 GO:0036376 IDA PMID:11916852; Q99271 NHA1 GO:0036376 IMP PMID:9802016; P27303 emrA GO:1990961
    IMP PMID:1409590; Q9LJX0 ABCB19 GO:0010315 IDA PMID:38513023; A0A254TZW7 cexA GO:0140115 IDA PMID:31062025.
    Per-set receipts are in the condition set notes.
confidence: 0.6
corrections:
- date: '2026-11-10'
  withdrawn_claim: >-
    The covered MFS, ABC and P-type ATPase transporters may import, export or work bidirectionally at
    various membranes, so direction and location cannot be inferred.
  correction: >-
    The claim was made about superfamily names without reading the condition sets. Each live set is a
    narrow conjunction whose reviewed members are experimentally annotated to export across the plasma
    membrane.
  error_type: UNEXAMINED_RULE
  evidence:
  - 'Previous version recorded condition_sets: []'
  - >-
    All 14 live sets have a Swiss-Prot match with an experimental annotation to GO:0140115 or a descendant
    (see set notes)
- date: '2026-11-10'
  withdrawn_claim: Literature contradicts the rule.
  correction: >-
    No contradicting paper was cited; the note said only that no direct literature was found. Curated
    experimental annotations support the term for members of every set.
  error_type: REFUTED_BY_CURATED_ANNOTATION
  evidence:
  - UniProtKB:Q7TMS5 GO:0140115 IDA PMID:20538997 (MGI)
  - UniProtKB:P05023 GO:0036376 IDA PMID:10636900 (BHF-UCL)
  - UniProtKB:P23895 GO:1990961 IDA PMID:24448799 (ComplexPortal)
- date: '2026-11-10'
  withdrawn_claim: >-
    Twelve condition sets covering different transporter families and taxa show that the rule lacks biological
    coherence.
  correction: >-
    Alternative condition sets for different families and taxa are how one term is assigned to several
    unrelated exporters; diversity among sets is not evidence that any annotation is wrong.
  error_type: SIZE_AS_EVIDENCE
  evidence:
  - 'Live rule: 14 condition sets, 12 with a taxon condition; per-set receipts in the set notes'
- date: '2026-11-10'
  withdrawn_claim: The term is mismatched because it is too specific for the transporters covered.
  correction: >-
    The reviewed members carry substrate-specific descendants of the term, so the rule term is the more
    general one.
  error_type: ONTOLOGY_MISREAD
  evidence:
  - >-
    QuickGO ancestors (is_a, part_of): GO:1990961, GO:0036376, GO:0010315, GO:1990034, GO:0097623 and
    GO:0033228 each have GO:0140115 as an ancestor
- date: '2026-11-10'
  withdrawn_claim: >-
    The assessments were supported by the rule's own manual deep-research note and by a file ARBA00043370.enriched.json.
  correction: >-
    Neither is an independent source and the enriched JSON file is not in the rule folder; those blocks
    were removed.
  error_type: UNSUPPORTED_SOURCE
  evidence:
  - rules/arba/ARBA00043370/ contains no ARBA00043370.enriched.json