ARBA00049204

id: ARBA00049204
description: Assigns the superoxide dismutase reaction 2 superoxide + 2 H(+) = H2O2 + O2 (RHEA:20696, EC 1.15.1.1)
  through 23 alternative condition sets. Sets 1 and 3 to 8 and the FunFams of superfamilies 1.10.287.990 and 3.55.40.20
  identify iron/manganese superoxide dismutases; set 2 and the FunFams of superfamily 2.60.40.200 identify copper/zinc
  superoxide dismutases. Most sets carry a taxon condition.
status: COMPLETE
rule_type: ARBA
rule:
  rule_id: ARBA00049204
  condition_sets:
  - number: 1
    conditions:
    - condition_type: INTERPRO
      value: IPR001189
      curie: InterPro:IPR001189
      label: Manganese/iron superoxide dismutase
      interpro_type: FAMILY
      negated: false
    - condition_type: INTERPRO
      value: IPR050265
      curie: InterPro:IPR050265
      label: Iron/Manganese Superoxide Dismutase
      interpro_type: FAMILY
      negated: false
    - condition_type: TAXON
      value: Eukaryota
      curie: NCBITaxon:2759
      label: Eukaryota
      negated: false
    notes: 'Swiss-Prot entries matching this set (2026-11-09): 54. 54 carry EC 1.15.1.1. UniProtKB entries matching:
      6102.'
  - number: 2
    conditions:
    - condition_type: INTERPRO
      value: IPR001424
      curie: InterPro:IPR001424
      label: Superoxide dismutase, copper/zinc binding domain
      interpro_type: DOMAIN
      negated: false
    - condition_type: INTERPRO
      value: IPR018152
      curie: InterPro:IPR018152
      label: Superoxide dismutase, copper/zinc, binding site
      interpro_type: BINDING_SITE
      negated: false
    - condition_type: TAXON
      value: Viridiplantae
      curie: NCBITaxon:33090
      label: Viridiplantae
      negated: false
    notes: 'Swiss-Prot entries matching this set (2026-11-09): 42. 42 carry EC 1.15.1.1. UniProtKB entries matching:
      2353.'
  - number: 3
    conditions:
    - condition_type: INTERPRO
      value: IPR019831
      curie: InterPro:IPR019831
      label: Manganese/iron superoxide dismutase, N-terminal
      interpro_type: DOMAIN
      negated: false
    - condition_type: INTERPRO
      value: IPR036324
      curie: InterPro:IPR036324
      label: Manganese/iron superoxide dismutase, N-terminal domain superfamily
      interpro_type: HOMOLOGOUS_SUPERFAMILY
      negated: false
    - condition_type: TAXON
      value: Metazoa
      curie: NCBITaxon:33208
      label: Metazoa
      negated: false
    notes: 'Swiss-Prot entries matching this set (2026-11-09): 29. 29 carry EC 1.15.1.1. UniProtKB entries matching:
      2205.'
  - number: 4
    conditions:
    - condition_type: INTERPRO
      value: IPR019832
      curie: InterPro:IPR019832
      label: Manganese/iron superoxide dismutase, C-terminal
      interpro_type: DOMAIN
      negated: false
    - condition_type: INTERPRO
      value: IPR036314
      curie: InterPro:IPR036314
      label: Manganese/iron superoxide dismutase, C-terminal domain superfamily
      interpro_type: HOMOLOGOUS_SUPERFAMILY
      negated: false
    - condition_type: TAXON
      value: Mycobacteriales
      curie: NCBITaxon:85007
      label: Mycobacteriales
      negated: false
    notes: 'Swiss-Prot entries matching this set (2026-11-09): 21. 21 carry EC 1.15.1.1. UniProtKB entries matching:
      783.'
  - number: 5
    conditions:
    - condition_type: INTERPRO
      value: IPR019833
      curie: InterPro:IPR019833
      label: Manganese/iron superoxide dismutase, binding site
      interpro_type: BINDING_SITE
      negated: false
    - condition_type: TAXON
      value: Enterobacterales
      curie: NCBITaxon:91347
      label: Enterobacterales
      negated: false
    notes: 'Swiss-Prot entries matching this set (2026-11-09): 16. 16 carry EC 1.15.1.1. UniProtKB entries matching:
      861.'
  - number: 6
    conditions:
    - condition_type: PANTHER
      value: PTHR42769
      curie: PANTHER:PTHR42769
      label: SUPEROXIDE DISMUTASE
      negated: false
    - condition_type: PANTHER
      value: PTHR42769:SF3
      curie: PANTHER:PTHR42769:SF3
      label: SUPEROXIDE DISMUTASE [FE] 2, CHLOROPLASTIC
      negated: false
    - condition_type: TAXON
      value: Gammaproteobacteria
      curie: NCBITaxon:1236
      label: Gammaproteobacteria
      negated: false
    notes: 'Swiss-Prot entries matching this set (2026-11-09): 13. 13 carry EC 1.15.1.1. UniProtKB entries matching:
      2555.'
  - number: 7
    conditions:
    - condition_type: PANTHER
      value: PTHR43595
      curie: PANTHER:PTHR43595
      label: 37S RIBOSOMAL PROTEIN S26, MITOCHONDRIAL
      negated: false
    - condition_type: PANTHER
      value: PTHR43595:SF2
      curie: PANTHER:PTHR43595:SF2
      label: SMALL RIBOSOMAL SUBUNIT PROTEIN MS42
      negated: false
    - condition_type: TAXON
      value: Bacillaceae
      curie: NCBITaxon:186817
      label: Bacillaceae
      negated: false
    notes: 'Swiss-Prot entries matching this set (2026-11-09): 7. 7 carry EC 1.15.1.1. UniProtKB entries matching:
      680.'
  - number: 8
    conditions:
    - condition_type: INTERPRO
      value: IPR054865
      curie: InterPro:IPR054865
      label: Superoxide dismutase [Mn], archaeal
      interpro_type: FAMILY
      negated: false
    - condition_type: TAXON
      value: Archaea
      curie: NCBITaxon:2157
      label: Archaea
      negated: false
    notes: 'Swiss-Prot entries matching this set (2026-11-09): 5. 5 carry EC 1.15.1.1. UniProtKB entries matching:
      348.'
  - number: 9
    conditions:
    - condition_type: FUNFAM
      value: 1.10.287.990:FF:000001
      curie: CATH.FunFam:1.10.287.990:FF:000001
      label: Superoxide dismutase
      negated: false
    - condition_type: TAXON
      value: Eukaryota
      curie: NCBITaxon:2759
      label: Eukaryota
      negated: false
    notes: 'Swiss-Prot entries matching this set (2026-11-09): 54. 54 carry EC 1.15.1.1. UniProtKB entries matching:
      5820.'
  - number: 10
    conditions:
    - condition_type: FUNFAM
      value: 2.60.40.200:FF:000001
      curie: CATH.FunFam:2.60.40.200:FF:000001
      label: Superoxide dismutase [Cu-Zn]
      negated: false
    - condition_type: TAXON
      value: Viridiplantae
      curie: NCBITaxon:33090
      label: Viridiplantae
      negated: false
    notes: 'Swiss-Prot entries matching this set (2026-11-09): 30. 30 carry EC 1.15.1.1. UniProtKB entries matching:
      1250.'
  - number: 11
    conditions:
    - condition_type: FUNFAM
      value: 1.10.287.990:FF:000002
      curie: CATH.FunFam:1.10.287.990:FF:000002
      label: Superoxide dismutase
      negated: false
    - condition_type: FUNFAM
      value: 3.55.40.20:FF:000001
      curie: CATH.FunFam:3.55.40.20:FF:000001
      label: Superoxide dismutase
      negated: false
    notes: 'Swiss-Prot entries matching this set (2026-11-09): 25. 25 carry EC 1.15.1.1. UniProtKB entries matching:
      4193.'
  - number: 12
    conditions:
    - condition_type: FUNFAM
      value: 3.55.40.20:FF:000002
      curie: CATH.FunFam:3.55.40.20:FF:000002
      label: Superoxide dismutase
      negated: false
    notes: 'Swiss-Prot entries matching this set (2026-11-09): 22. 22 carry EC 1.15.1.1. UniProtKB entries matching:
      2612.'
  - number: 13
    conditions:
    - condition_type: FUNFAM
      value: 3.55.40.20:FF:000004
      curie: CATH.FunFam:3.55.40.20:FF:000004
      label: Superoxide dismutase [Fe]
      negated: false
    - condition_type: TAXON
      value: Mycobacteriales
      curie: NCBITaxon:85007
      label: Mycobacteriales
      negated: false
    notes: 'Swiss-Prot entries matching this set (2026-11-09): 15. 15 carry EC 1.15.1.1. UniProtKB entries matching:
      733.'
  - number: 14
    conditions:
    - condition_type: FUNFAM
      value: 2.60.40.200:FF:000003
      curie: CATH.FunFam:2.60.40.200:FF:000003
      label: Superoxide dismutase [Cu-Zn], chloroplastic
      negated: false
    notes: 'Swiss-Prot entries matching this set (2026-11-09): 14. 14 carry EC 1.15.1.1. UniProtKB entries matching:
      1545.'
  - number: 15
    conditions:
    - condition_type: FUNFAM
      value: 2.60.40.200:FF:000002
      curie: CATH.FunFam:2.60.40.200:FF:000002
      label: Superoxide dismutase [Cu-Zn]
      negated: false
    - condition_type: TAXON
      value: Enterobacterales
      curie: NCBITaxon:91347
      label: Enterobacterales
      negated: false
    notes: 'Swiss-Prot entries matching this set (2026-11-09): 5. 5 carry EC 1.15.1.1. UniProtKB entries matching:
      208.'
  - number: 16
    conditions:
    - condition_type: FUNFAM
      value: 2.60.40.200:FF:000012
      curie: CATH.FunFam:2.60.40.200:FF:000012
      label: Superoxide dismutase [Cu-Zn]
      negated: false
    - condition_type: TAXON
      value: Bacteria
      curie: NCBITaxon:2
      label: Bacteria
      negated: false
    notes: 'Swiss-Prot entries matching this set (2026-11-09): 5. 5 carry EC 1.15.1.1. UniProtKB entries matching:
      361.'
  - number: 17
    conditions:
    - condition_type: FUNFAM
      value: 3.55.40.20:FF:000005
      curie: CATH.FunFam:3.55.40.20:FF:000005
      label: Superoxide dismutase
      negated: false
    - condition_type: TAXON
      value: Streptophyta
      curie: NCBITaxon:35493
      label: Streptophyta
      negated: false
    notes: 'Swiss-Prot entries matching this set (2026-11-09): 5. 5 carry EC 1.15.1.1. UniProtKB entries matching:
      704.'
  - number: 18
    conditions:
    - condition_type: FUNFAM
      value: 2.60.40.200:FF:000007
      curie: CATH.FunFam:2.60.40.200:FF:000007
      label: Cell surface Cu-only superoxide dismutase 5
      negated: false
    - condition_type: TAXON
      value: Fungi
      curie: NCBITaxon:4751
      label: Fungi
      negated: false
    notes: 'Swiss-Prot entries matching this set (2026-11-09): 3. 3 carry EC 1.15.1.1. UniProtKB entries matching:
      1061.'
  - number: 19
    conditions:
    - condition_type: FUNFAM
      value: 2.60.120.10:FF:000025
      curie: CATH.FunFam:2.60.120.10:FF:000025
      label: germin-like protein subfamily 2 member 1
      negated: false
    - condition_type: TAXON
      value: asterids
      curie: NCBITaxon:71274
      label: asterids
      negated: false
    notes: 'Swiss-Prot entries matching this set (2026-11-09): 2. 2 carry EC 1.15.1.1. UniProtKB entries matching:
      715.'
  - number: 20
    conditions:
    - condition_type: FUNFAM
      value: 2.60.40.200:FF:000005
      curie: CATH.FunFam:2.60.40.200:FF:000005
      label: Superoxide dismutase [Cu-Zn]
      negated: false
    - condition_type: TAXON
      value: Pseudomonadati
      curie: NCBITaxon:3379134
      label: Pseudomonadati
      negated: false
    notes: 'Swiss-Prot entries matching this set (2026-11-09): 2. 2 carry EC 1.15.1.1. UniProtKB entries matching:
      10.'
  - number: 21
    conditions:
    - condition_type: FUNFAM
      value: 3.55.40.20:FF:000006
      curie: CATH.FunFam:3.55.40.20:FF:000006
      label: Superoxide dismutase
      negated: false
    - condition_type: TAXON
      value: Bacillati
      curie: NCBITaxon:1783272
      label: Bacillati
      negated: false
    notes: 'Swiss-Prot entries matching this set (2026-11-09): 2. 2 carry EC 1.15.1.1. UniProtKB entries matching:
      19.'
  - number: 22
    conditions:
    - condition_type: FUNFAM
      value: 3.55.40.20:FF:000008
      curie: CATH.FunFam:3.55.40.20:FF:000008
      label: Superoxide dismutase
      negated: false
    - condition_type: TAXON
      value: Campylobacterota
      curie: NCBITaxon:29547
      label: Campylobacterota
      negated: false
    notes: 'Swiss-Prot entries matching this set (2026-11-09): 2. 2 carry EC 1.15.1.1. UniProtKB entries matching:
      23.'
  - number: 23
    conditions:
    - condition_type: FUNFAM
      value: 2.60.40.200:FF:000013
      curie: CATH.FunFam:2.60.40.200:FF:000013
      label: Superoxide dismutase [Cu-Zn]
      negated: false
    notes: 'Swiss-Prot entries matching this set (2026-11-09): 2. 2 carry EC 1.15.1.1. UniProtKB entries matching:
      250.'
  go_annotations: []
  catalytic_activity_annotations:
  - reaction_name: 2 superoxide + 2 H(+) = H2O2 + O2
    rhea_id: RHEA:20696
    ec_number: 1.15.1.1
  entries: []
  reviewed_protein_count: 0
  unreviewed_protein_count: 19126
  created_date: '2024-10-21'
  modified_date: '2026-05-04'
  retrieved_date: '2026-11-09'
  source_condition_set_count: 23
review_summary: All 23 live condition sets were checked against Swiss-Prot. Each has at least two reviewed matches
  (2 to 54 per set) and every reviewed match of every set carries EC 1.15.1.1. Checked against Swiss-Prot by UniProtKB
  query on 2026-11-09; TrEMBL matches were counted but not inspected.
action: ACCEPT
action_rationale: 'Every live condition set is supported by curated Swiss-Prot annotation: no reviewed match of
  any of the 23 sets lacks EC 1.15.1.1.'
taxonomic_scope:
  assessment: APPROPRIATE
  notes: 'The taxon conditions go with precise sets. The copper/zinc binding domain alone is not specific: of 195
    Swiss-Prot proteins with IPR001424, 12 lack EC 1.15.1.1, and IPR024134 is named "Superoxide dismutase (Cu/Zn)/superoxide
    dismutase copper chaperone". The live sets that use copper/zinc signatures match only EC 1.15.1.1 proteins in
    Swiss-Prot (set 2: 42 of 42).'
annotation_assessments:
- annotation_kind: CATALYTIC_ACTIVITY
  value: RHEA:20696
  label: 2 superoxide + 2 H(+) = H2O2 + O2 (EC 1.15.1.1)
  assessment: CORRECT
  correct_examples:
  - P04179
  - P09671
  - P24704
  - O78310
  - P07505
  - P09678
  notes: 'Swiss-Prot matches with EC 1.15.1.1, sets 1 to 23: 54/54, 42/42, 29/29, 21/21, 16/16, 13/13, 7/7, 5/5,
    54/54, 30/30, 25/25, 22/22, 15/15, 14/14, 5/5, 5/5, 5/5, 3/3, 2/2, 2/2, 2/2, 2/2, 2/2. Rule statistics: 19126
    unreviewed proteins.'
confidence: 0.85
corrections:
- date: '2026-11-10'
  withdrawn_claim: The rule needs modification because its 24 condition sets exceed a manageable threshold of 12
    and make it intractable to validate.
  correction: The number of condition sets says nothing about whether the annotations are right. Checked set by
    set, every one is supported.
  error_type: SIZE_AS_EVIDENCE
  evidence:
  - 'Live rule: 23 condition sets (2026-11-09)'
  - Swiss-Prot matches of each set all carry EC 1.15.1.1 (per-set counts in annotation_assessments)
- date: '2026-11-10'
  withdrawn_claim: Condition set 3 (IPR019831 with IPR036324, Metazoa) is the copper/zinc superoxide dismutase of
    animals.
  correction: Both entries are iron/manganese signatures, so set 3 identifies the mitochondrial Mn superoxide dismutase
    (e.g. human SOD2, P04179).
  error_type: WRONG_IDENTIFIER_MEANING
  evidence:
  - 'InterPro IPR019831: Manganese/iron superoxide dismutase, N-terminal (domain)'
  - 'InterPro IPR036324: Manganese/iron superoxide dismutase, N-terminal domain superfamily'
  - Swiss-Prot matches of CS3 include P04179 (SOD2, human)
- date: '2026-11-10'
  withdrawn_claim: IPR001189 is the manganese/iron superoxide dismutase alpha-hairpin domain and IPR050265 its C-terminal
    domain; IPR019832 and IPR036314 are an iron/manganese family and its alpha-hairpin domain.
  correction: These labels were wrong; the official names are given in evidence.
  error_type: WRONG_IDENTIFIER_MEANING
  evidence:
  - 'InterPro IPR001189: Manganese/iron superoxide dismutase (family)'
  - 'InterPro IPR050265: Iron/Manganese Superoxide Dismutase (family)'
  - 'InterPro IPR019832: Manganese/iron superoxide dismutase, C-terminal (domain)'
  - 'InterPro IPR036314: Manganese/iron superoxide dismutase, C-terminal domain superfamily'
- date: '2026-11-10'
  withdrawn_claim: A separate copper/zinc set for Endopterygota is redundant with the broader Metazoa set.
  correction: The Metazoa set is an iron/manganese set, so it could not have made a copper/zinc set redundant. The
    live rule has no Endopterygota set.
  error_type: STALE_RULE_VERSION
  evidence:
  - 'Live rule (2026-11-09): no condition with taxon Endopterygota; CS3 = IPR019831 AND IPR036324 AND Metazoa'
- date: '2026-11-10'
  withdrawn_claim: Broader taxonomic groups should replace the specific lineages, because the subdivision has no
    biological justification.
  correction: No check of precision under broader taxa was made. The copper/zinc domain also occurs in proteins
    without superoxide dismutase activity, so widening is not shown to be safe.
  error_type: TAXON_MISREAD
  evidence:
  - 'UniProtKB query xref:interpro-IPR001424 AND reviewed:true: 195 entries; 12 without ec:1.15.1.1'
  - 'InterPro IPR024134: Superoxide dismutase (Cu/Zn)/superoxide dismutase copper chaperone'