View original ARBA rule on UniProt
Assigns GO:0043935 (sexual sporulation resulting in formation of a cellular spore) through four alternative condition sets, each a CATH FunFam with a taxon restriction: 14-3-3 proteins in Saccharomycetes, a syntaxin FunFam in Saccharomycetales, a chitin deacetylase FunFam in Eukaryota excluding Dictyostelia, and the sporulation protein kinase mde3 FunFam in Fungi.
Condition-set counts describe the sets recorded in this review, which may omit the full rule.
No domain overlap table is shown: the cached overlap analysis for this version of the review is not available.
Run just analyze-rule ARBA00086620 and re-render to add it.
All four live condition sets were examined. Each matches two reviewed proteins, and all eight carry an experimentally supported annotation to a descendant of GO:0043935 (ascospore formation, ascospore wall assembly or prospore assembly). The rule is sound for the proteins that can be checked.
Every condition set selects reviewed fungal proteins with direct experimental evidence for a role in ascospore formation. The four sets are alternatives and are expected to select different protein families. The counts are from Swiss-Prot, which is also what ARBA learns its rules from, so they show the condition sets are coherent rather than giving an independent error rate for unreviewed entries.
Set 1: P29311 (BMH1) and P34730 (BMH2), GO:0030437 ascospore formation, IGI, PMID:9215628. Set 2: P32867 (SSO1), GO:0030437, IMP, PMID:11839791; P39926 (SSO2), GO:0031321 ascospore-type prospore assembly, IGI, PMID:19502581. Set 3: Q06702 (CDA1) and Q06703 (CDA2), GO:0030476 ascospore wall assembly, IMP, PMID:10544249. Set 4: O14132 (pit1) IMP and O43077 (mde3) IGI, GO:0030437, PMID:10747048. All three terms have GO:0043935 as an ancestor (QuickGO). Source: QuickGO annotation search, retrieved 2026-11-09.
UniProtKB release 2026_04: 2 reviewed entries match this set; 2 of them carry GO:0043935 or a descendant, 2 of those by a manual-evidence GO annotation. 80 unreviewed entries match.
UniProtKB release 2026_04: 2 reviewed entries match this set; 2 of them carry GO:0043935 or a descendant, 2 of those by a manual-evidence GO annotation. 62 unreviewed entries match.
UniProtKB release 2026_04: 2 reviewed entries match this set; 2 of them carry GO:0043935 or a descendant, 2 of those by a manual-evidence GO annotation. 70 unreviewed entries match.
UniProtKB release 2026_04: 2 reviewed entries match this set; 2 of them carry GO:0043935 or a descendant, 2 of those by a manual-evidence GO annotation. 0 unreviewed entries match.
Three of the four sets are restricted to fungal taxa. The chitin deacetylase set allows Eukaryota except Dictyostelia, but its two reviewed matches and its FunFam are fungal (Saccharomyces cerevisiae CDA1 and CDA2).
Reference for the IMP annotations of GO:0030476 on CDA1 and CDA2.
Reference for the annotations of GO:0030437 on pit1 and mde3.
id: ARBA00086620
description: >-
Assigns GO:0043935 (sexual sporulation resulting in formation of a cellular spore) through four
alternative condition sets, each a CATH FunFam with a taxon restriction: 14-3-3 proteins in Saccharomycetes,
a syntaxin FunFam in Saccharomycetales, a chitin deacetylase FunFam in Eukaryota excluding Dictyostelia,
and the sporulation protein kinase mde3 FunFam in Fungi.
status: COMPLETE
rule_type: ARBA
rule:
rule_id: ARBA00086620
condition_sets:
- number: 1
conditions:
- condition_type: FUNFAM
value: 1.20.190.20:FF:000002
curie: CATH.FunFam:1.20.190.20:FF:000002
label: 14-3-3 protein epsilon
negated: false
- condition_type: TAXON
value: Saccharomycetes
curie: NCBITaxon:4891
label: Saccharomycetes
negated: false
notes: >-
UniProtKB release 2026_04: 2 reviewed entries match this set; 2 of them carry GO:0043935 or
a descendant, 2 of those by a manual-evidence GO annotation. 80 unreviewed entries match.
- number: 2
conditions:
- condition_type: FUNFAM
value: 1.20.58.70:FF:000008
curie: CATH.FunFam:1.20.58.70:FF:000008
label: Syntaxin family protein
negated: false
- condition_type: TAXON
value: Saccharomycetales
curie: NCBITaxon:4892
label: Saccharomycetales
negated: false
notes: >-
UniProtKB release 2026_04: 2 reviewed entries match this set; 2 of them carry GO:0043935 or
a descendant, 2 of those by a manual-evidence GO annotation. 62 unreviewed entries match.
- number: 3
conditions:
- condition_type: FUNFAM
value: 3.20.20.370:FF:000008
curie: CATH.FunFam:3.20.20.370:FF:000008
label: Chitin deacetylase
negated: false
- condition_type: TAXON
value: Eukaryota
curie: NCBITaxon:2759
label: Eukaryota
negated: false
- condition_type: TAXON
value: Dictyostelia
curie: NCBITaxon:33083
label: Dictyostelia
negated: true
notes: >-
UniProtKB release 2026_04: 2 reviewed entries match this set; 2 of them carry GO:0043935 or
a descendant, 2 of those by a manual-evidence GO annotation. 70 unreviewed entries match.
- number: 4
conditions:
- condition_type: FUNFAM
value: 3.30.200.20:FF:001493
curie: CATH.FunFam:3.30.200.20:FF:001493
label: Sporulation protein kinase mde3
negated: false
- condition_type: TAXON
value: Fungi
curie: NCBITaxon:4751
label: Fungi
negated: false
notes: >-
UniProtKB release 2026_04: 2 reviewed entries match this set; 2 of them carry GO:0043935 or
a descendant, 2 of those by a manual-evidence GO annotation. 0 unreviewed entries match.
go_annotations:
- go_id: GO:0043935
go_label: sexual sporulation resulting in formation of a cellular spore
aspect: BP
entries:
- id: 1.20.190.20:FF:000002
type: FUNFAM
label: 14-3-3 protein epsilon
appears_in_condition_sets:
- 1
protein_count: 69
related_entries:
- relationship: EQUIV
target_id: 3.30.200.20:FF:001493
containment: 0.0
jaccard_similarity: 0.0
intersection_count: 0
exclusive_count: 69
- relationship: EQUIV
target_id: 1.20.58.70:FF:000008
containment: 0.0
jaccard_similarity: 0.0
intersection_count: 0
exclusive_count: 69
- relationship: EQUIV
target_id: 3.20.20.370:FF:000008
containment: 0.0
jaccard_similarity: 0.0
intersection_count: 0
exclusive_count: 69
- relationship: EQUIV
target_id: GO:0043935
containment: 0.029
jaccard_similarity: 0.006
intersection_count: 2
exclusive_count: 67
- id: 1.20.58.70:FF:000008
type: FUNFAM
label: Syntaxin family protein
appears_in_condition_sets:
- 2
protein_count: 4
related_entries:
- relationship: EQUIV
target_id: 3.30.200.20:FF:001493
containment: 0.0
jaccard_similarity: 0.0
intersection_count: 0
exclusive_count: 4
- relationship: EQUIV
target_id: 1.20.190.20:FF:000002
containment: 0.0
jaccard_similarity: 0.0
intersection_count: 0
exclusive_count: 4
- relationship: EQUIV
target_id: 3.20.20.370:FF:000008
containment: 0.0
jaccard_similarity: 0.0
intersection_count: 0
exclusive_count: 4
- relationship: PREDICTS
target_id: GO:0043935
containment: 0.75
jaccard_similarity: 0.011
intersection_count: 3
exclusive_count: 1
- id: 3.20.20.370:FF:000008
type: FUNFAM
label: Chitin deacetylase
appears_in_condition_sets:
- 3
protein_count: 2
related_entries:
- relationship: EQUIV
target_id: 3.30.200.20:FF:001493
containment: 0.0
jaccard_similarity: 0.0
intersection_count: 0
exclusive_count: 2
- relationship: EQUIV
target_id: 1.20.190.20:FF:000002
containment: 0.0
jaccard_similarity: 0.0
intersection_count: 0
exclusive_count: 2
- relationship: EQUIV
target_id: 1.20.58.70:FF:000008
containment: 0.0
jaccard_similarity: 0.0
intersection_count: 0
exclusive_count: 2
- relationship: PREDICTS
target_id: GO:0043935
containment: 1.0
jaccard_similarity: 0.008
intersection_count: 2
exclusive_count: 0
- id: 3.30.200.20:FF:001493
type: FUNFAM
label: Sporulation protein kinase mde3
appears_in_condition_sets:
- 4
protein_count: 2
related_entries:
- relationship: EQUIV
target_id: 1.20.190.20:FF:000002
containment: 0.0
jaccard_similarity: 0.0
intersection_count: 0
exclusive_count: 2
- relationship: EQUIV
target_id: 1.20.58.70:FF:000008
containment: 0.0
jaccard_similarity: 0.0
intersection_count: 0
exclusive_count: 2
- relationship: EQUIV
target_id: 3.20.20.370:FF:000008
containment: 0.0
jaccard_similarity: 0.0
intersection_count: 0
exclusive_count: 2
- relationship: PREDICTS
target_id: GO:0043935
containment: 1.0
jaccard_similarity: 0.008
intersection_count: 2
exclusive_count: 0
reviewed_protein_count: 0
unreviewed_protein_count: 0
created_date: '2025-03-21'
modified_date: '2026-07-18'
retrieved_date: '2026-11-09'
source_condition_set_count: 4
review_summary: >-
All four live condition sets were examined. Each matches two reviewed proteins, and all eight carry
an experimentally supported annotation to a descendant of GO:0043935 (ascospore formation, ascospore
wall assembly or prospore assembly). The rule is sound for the proteins that can be checked.
action: ACCEPT
action_rationale: >-
Every condition set selects reviewed fungal proteins with direct experimental evidence for a role
in ascospore formation. The four sets are alternatives and are expected to select different protein
families. The counts are from Swiss-Prot, which is also what ARBA learns its rules from, so they
show the condition sets are coherent rather than giving an independent error rate for unreviewed
entries.
taxonomic_scope:
assessment: APPROPRIATE
notes: >-
Three of the four sets are restricted to fungal taxa. The chitin deacetylase set allows Eukaryota
except Dictyostelia, but its two reviewed matches and its FunFam are fungal (Saccharomyces cerevisiae
CDA1 and CDA2).
annotation_assessments:
- annotation_kind: GO_TERM
value: GO:0043935
label: sexual sporulation resulting in formation of a cellular spore
assessment: CORRECT
matched_protein_count: 8
incorrect_protein_count: 0
correct_examples:
- P29311
- P34730
- P32867
- P39926
- Q06702
- Q06703
- O14132
- O43077
notes: >-
Set 1: P29311 (BMH1) and P34730 (BMH2), GO:0030437 ascospore formation, IGI, PMID:9215628. Set
2: P32867 (SSO1), GO:0030437, IMP, PMID:11839791; P39926 (SSO2), GO:0031321 ascospore-type prospore
assembly, IGI, PMID:19502581. Set 3: Q06702 (CDA1) and Q06703 (CDA2), GO:0030476 ascospore wall
assembly, IMP, PMID:10544249. Set 4: O14132 (pit1) IMP and O43077 (mde3) IGI, GO:0030437, PMID:10747048.
All three terms have GO:0043935 as an ancestor (QuickGO). Source: QuickGO annotation search, retrieved
2026-11-09.
confidence: 0.75
corrections:
- date: '2026-11-10'
withdrawn_claim: >-
There is no support for the claim that MAPK, 14-3-3, syntaxin, chitin deacetylase and dynein light
chain proteins collectively predict sexual sporulation; the families lack a common mechanism.
correction: >-
The rule makes no collective claim. Its condition sets are alternatives, each sufficient on its
own, and each selects proteins with experimental evidence for ascospore formation.
error_type: CONJUNCTION_IGNORED
evidence:
- >-
UniProt ARBA API, ARBA00086620: four condition sets, each one FunFam plus taxon conditions.
- >-
QuickGO: experimental annotations to descendants of GO:0043935 on all eight reviewed matches (accessions
in annotation_assessments).
- date: '2026-11-10'
withdrawn_claim: >-
The low overlap between condition sets (average Jaccard 0.006) shows the rule groups unrelated
protein populations and is incoherent.
correction: >-
Alternative condition sets are meant to select different proteins. Their disjointness says nothing
about whether each annotation is true.
error_type: OTHER
evidence:
- 'UniProt ARBA API: the consequent applies when any one condition set is satisfied.'
- date: '2026-11-10'
withdrawn_claim: The rule matches 0 proteins, which indicates unrealistic condition combinations.
correction: >-
The zero was the reviewed-protein statistic that the ARBA API reports for the rule, not a count
of matches. The condition sets match reviewed and unreviewed UniProtKB entries.
error_type: OTHER
evidence:
- >-
UniProtKB release 2026_04: the four sets match 2 reviewed entries each and 80, 62, 70 and 0 unreviewed
entries respectively.
- date: '2026-11-10'
withdrawn_claim: >-
The rule applies a fungal process to all eukaryotes, which would cause massive over-annotation.
correction: >-
The live rule has no set that is open to all eukaryotes on a general domain. The only non-fungal
taxon condition is on the chitin deacetylase FunFam, which matches 2 reviewed and 70 unreviewed
entries. The earlier Eukaryota-wide kinase set required the mde3 FunFam, which had two Swiss-Prot
members, both Schizosaccharomyces pombe sporulation kinases; it is now restricted to Fungi.
error_type: TAXON_MISREAD
evidence:
- >-
UniProt ARBA API, ARBA00086620, modified 2026-07-18: taxon conditions Saccharomycetes, Saccharomycetales,
Eukaryota NOT Dictyostelia, Fungi.
- >-
QuickGO: O14132 (pit1) GO:0030437 IMP PMID:10747048; O43077 (mde3) GO:0030437 IGI PMID:10747048.
- date: '2026-11-10'
withdrawn_claim: The condition sets include a MAPK FunFam and a dynein light chain FunFam.
correction: Neither is in the live rule, which has four sets.
error_type: STALE_RULE_VERSION
evidence:
- >-
UniProt ARBA API, ARBA00086620, modified 2026-07-18: no condition on 1.10.510.10:FF:000624 or
3.30.740.10:FF:000001.
references:
- id: PMID:10544249
title: Yeast ascospore wall assembly requires two chitin deacetylase isozymes
findings:
- statement: Reference for the IMP annotations of GO:0030476 on CDA1 and CDA2.
- id: PMID:10747048
title: >-
Autoregulated expression of Schizosaccharomyces pombe meiosis-specific transcription factor Mei4
and a genome-wide search for its target genes
findings:
- statement: Reference for the annotations of GO:0030437 on pit1 and mde3.