ARBA00090047 protein localization to mitotic actomyosin contractile ring (GO:1904498)

id: ARBA00090047
description: >-
  Withdrawn rule. As last recorded it assigned GO:1904498 (protein localization to mitotic actomyosin
  contractile ring) to Taphrinomycotina proteins in CATH FunFam 1.20.58.2220:FF:000006 (Cytokinesis protein
  sepA), the family of S. pombe for3 (Formin-3) and cdc12.
status: COMPLETE
rule_type: ARBA
rule:
  rule_id: ARBA00090047
  condition_sets:
  - number: 1
    conditions:
    - condition_type: FUNFAM
      value: 1.20.58.2220:FF:000006
      curie: CATH.FunFam:1.20.58.2220:FF:000006
      label: Cytokinesis protein sepA
      negated: false
    - condition_type: TAXON
      value: Taphrinomycotina
      curie: NCBITaxon:451866
      label: Taphrinomycotina
      negated: false
    notes: >-
      Condition set as recorded before the rule was withdrawn; it could not be re-read from UniProt. Swiss-Prot
      entries with this FunFam in Taphrinomycotina (UniProtKB query, 2026-11-10): O94532 (for3) and Q10059
      (cdc12), both from S. pombe; 9 unreviewed entries. Neither has an annotation to GO:1904498; both
      have experimental annotations to GO:1903475 mitotic actomyosin contractile ring assembly (O94532
      IGI PMID:24127216, Q10059 IMP PMID:12214236, PomBase).
  go_annotations:
  - go_id: GO:1904498
    go_label: protein localization to mitotic actomyosin contractile ring
    aspect: BP
  entries: []
  retrieved_date: '2026-11-09'
  withdrawn: true
review_summary: >-
  UniProt returned HTTP 404 for this rule on 2026-11-09, so it has been withdrawn. The condition set and
  the GO term are those recorded in the previous version of this review and could not be re-read. The
  FunFam is named for Aspergillus nidulans sepA (P78621); its Swiss-Prot members in Taphrinomycotina are
  S. pombe for3 (O94532, Formin-3) and cdc12 (Q10059). No protein in Taphrinomycotina has a curated annotation
  to GO:1904498.
action: DEPRECATE
action_rationale: The rule is no longer served by UniProt. No replacement is proposed.
annotation_assessments:
- annotation_kind: GO_TERM
  value: GO:1904498
  label: protein localization to mitotic actomyosin contractile ring
  assessment: UNDECIDED
  notes: >-
    QuickGO returns no annotation to GO:1904498 or its descendants for any Taphrinomycotina protein (taxon
    451866 and descendants, 2026-11-10), and none for O94532 or Q10059. The experimental annotations to
    the exact term are in S. cerevisiae: P08964 MYO1 IDA PMID:15210731 and Q06604 BSP1 IMP PMID:38088874
    (SGD).
confidence: 0.9
corrections:
- date: '2026-11-10'
  withdrawn_claim: SepA proteins are well-established cytokinesis regulators in Schizosaccharomyces pombe.
  correction: >-
    sepA is an Aspergillus nidulans protein. The S. pombe members of the FunFam are for3 (Formin-3) and
    cdc12.
  error_type: WRONG_IDENTIFIER_MEANING
  evidence:
  - UniProtKB:P78621 sepA, Aspergillus nidulans, Cytokinesis protein sepA
  - >-
    Swiss-Prot entries with FunFam 1.20.58.2220:FF:000006 in Taphrinomycotina: O94532 (for3, Formin-3),
    Q10059 (cdc12)
- date: '2026-11-10'
  withdrawn_claim: >-
    Consider GO:0032147 (activation of protein kinase activity) if SepA acts as a kinase regulator.
  correction: >-
    The S. pombe members are for3 (Formin-3) and cdc12; nothing recorded supports a kinase-regulator role.
  error_type: UNSUPPORTED_SOURCE
  evidence:
  - 'UniProtKB:O94532 recommended name: Formin-3'
  - 'QuickGO GO:0032147 label: activation of protein kinase activity'
- date: '2026-11-10'
  withdrawn_claim: >-
    The term requires evidence that the annotated protein itself localizes to the contractile ring.
  correction: >-
    The term is a process: a protein is annotated to it when it takes part in localizing proteins to the
    ring.
  error_type: ONTOLOGY_MISREAD
  evidence:
  - >-
    QuickGO GO:1904498 definition: Any protein localization to actomyosin contractile ring that is involved
    in mitotic cytokinesis
  - UniProtKB:Q06604 BSP1 GO:1904498 IMP PMID:38088874 (SGD)
- date: '2026-11-10'
  withdrawn_claim: The rule is well designed and its restriction to Taphrinomycotina is appropriate.
  correction: >-
    This was asserted without checking which proteins the set matches or whether any carries the term;
    none does. The rule has since been withdrawn.
  error_type: UNEXAMINED_RULE
  evidence:
  - https://rest.uniprot.org/arba/ARBA00090047 returned HTTP 404 on 2026-11-09
  - No GO:1904498 annotation in Taphrinomycotina (QuickGO, 2026-11-10)
- date: '2026-11-10'
  withdrawn_claim: >-
    Assessments were supported by the rule's own manual deep-research note and by ARBA00090047-analysis.yaml.
  correction: >-
    The note is not an independent source and the analysis file is not in the rule folder; those blocks
    were removed.
  error_type: UNSUPPORTED_SOURCE
  evidence:
  - >-
    rules/arba/ARBA00090047/ contains only the deep-research-manual note, the review YAML and its HTML
    rendering