S0EDH7

UniProt ID: S0EDH7
Organism: Gibberella fujikuroi (strain CBS 195.34 / IMI 58289 / NRRL A-6831)
Review Status: DRAFT
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Gene Description

FFUJ_06423 is a 342-amino-acid uncharacterized protein from the phytopathogenic ascomycete Fusarium fujikuroi (Gibberella fujikuroi). It contains a protein kinase-like domain fold identified by three independent structural classification methods: InterPro IPR011009 (Kinase-like domain superfamily), Gene3D 1.10.510.10 (Phosphotransferase domain), and SUPFAM SSF56112 (Protein kinase-like). The convergent domain evidence predicts that FFUJ_06423 functions as a protein kinase, catalyzing the transfer of a phosphoryl group from ATP to protein substrates. However, no specific kinase subfamily assignment, substrate, regulatory partner, or signaling pathway membership has been established. No experimental characterization has been reported (UniProt PE level 4: Predicted). The protein was identified from the F. fujikuroi CBS 195.34 genome sequencing project.

Core Functions

Based on the presence of a kinase-like domain fold (InterPro IPR011009, Gene3D 1.10.510.10, SUPFAM SSF56112), FFUJ_06423 is predicted to function as a protein kinase that catalyzes the phosphorylation of protein substrates using ATP as the phosphoryl group donor. No specific kinase subfamily or substrate has been determined.

Molecular Function:
protein kinase activity
Directly Involved In:
protein phosphorylation
Supporting Evidence:
  • file:GIBF5/S0EDH7/S0EDH7-uniprot.txt
    InterPro; IPR011009; Kinase-like_dom_sf. Gene3D; 1.10.510.10; Transferase(Phosphotransferase) domain 1; 1. SUPFAM; SSF56112; Protein kinase-like (PK-like); 1.
  • file:GIBF5/S0EDH7/S0EDH7-protnlm-predictions-review.yaml
    ProtNLM2 predicted protein phosphorylation (GO:0006468) and ATP binding (GO:0005524), both assessed as COR (correct novel) based on convergent domain evidence

References

PMID:23825955
Deciphering the cryptic genome: genome-wide analyses of the rice pathogen Fusarium fujikuroi reveal complex regulation of secondary metabolism and novel metabolites

Suggested Questions for Experts

Q: What specific kinase subfamily does FFUJ_06423 belong to, and can sequence comparison to characterized fungal kinases narrow the substrate specificity?

Q: Is FFUJ_06423 part of a known signaling module (e.g., MAPK cascade, TOR pathway, or cAMP-PKA pathway) in Fusarium fujikuroi?

Q: Is FFUJ_06423 essential for growth, or is it dispensable under standard laboratory conditions?

Suggested Experiments

Experiment: Express and purify recombinant FFUJ_06423, then assay kinase activity using radiolabeled ATP and generic substrates such as myelin basic protein or casein. Autophosphorylation can be tested in the absence of exogenous substrates.

Hypothesis: FFUJ_06423 encodes a functional protein kinase that can autophosphorylate or phosphorylate generic substrates in vitro.

Experiment: Construct a targeted FFUJ_06423 deletion mutant using homologous recombination and compare growth rate, conidiation, pigment production, gibberellin biosynthesis, and rice pathogenicity to the wild-type CBS 195.34 strain.

Hypothesis: Deletion of FFUJ_06423 affects growth, morphology, or pathogenicity in Fusarium fujikuroi.

External Prediction Reviews

These computational predictions are reviewed separately from the GOA annotation set used for this review. The assessments below are from this project and do not constitute official GO annotations or endorsement by GO/UniProt. They are not included in the existing annotation review above.

ProtNLM2 External predictions

View prediction review YAML · S0EDH7-protnlm-predictions-review.yaml · Review status: COMPLETE

A broad kinase-like fold assignment does not establish ATP binding or protein phosphorylation for this uncharacterized fungal protein.

Source documents: genes/GIBF5/S0EDH7/S0EDH7-uniprot.txt · genes/GIBF5/S0EDH7/S0EDH7-goa.tsv

Review score: 2 = concordant with evidence; 1 = uncertain; 0 = discordant with evidence. This is an assessment score, not a model probability.

GO:0006468 protein phosphorylation GO_BP
UNC — Uncertain Review score: 1/2
Prediction method: ProtNLM2 · Version: UniProt 2024_06 pilot
Review rationale: The only InterPro assignment is the broad kinase-like structural superfamily IPR011009. The record does not identify a canonical protein kinase domain or its diagnostic catalytic-site signatures, and the inspected sources contain no protein-substrate phosphorylation assay. A kinase-like fold can support a hypothesis but cannot establish this exact process. Protein phosphorylation is absent from the cached annotations and remains uncertain.
Supporting Evidence:
GO:0005524 ATP binding GO_MF
UNC — Uncertain Review score: 1/2
Prediction method: ProtNLM2 · Version: UniProt 2024_06 pilot
Review rationale: The kinase-like structural superfamily assignment is compatible with nucleotide binding in some members, but it does not establish ATP recognition by this protein. The inspected record contains no diagnostic ATP-binding-site assignment or experimentally characterized close relative establishing that property. ATP binding is absent from the cached annotations. Ligand-binding evidence or a more specific, supported functional-family assignment is needed.
Supporting Evidence:

Deep Research

Falcon

(S0EDH7-deep-research-falcon.md)

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