Conserved-unknown protein (PomBase gene name asi1/ceo6) orthologous to human neurochondrin (NCDN, HGNC:17597). Carries a neurochondrin / ARM-type-fold domain architecture (Pfam PF05536, PANTHER NEUROCHONDRIN, SUPERFAMILY ARM repeat) and is predicted to act as a scaffolding/adaptor protein. Curated binary interactions include the spindle pole body component Sfi1, the cortical dynein anchor Num1/Mcp5, the chronological-lifespan regulator Ecl1, Pof4 and Rng10, implicating it in spindle pole body, cortical microtubule and cellular aging contexts. Falcon deep research surfaced Tay et al. 2013 (PMID:23365689) and reported SPCC16C4.02c as an Ino80 chromatin-remodeler subunit implicated in doxorubicin resistance; this is an artifact. The Ino80-complex membership and the deletion/epistasis experiments in that paper concern the gene Spcc16c4.20c, which is a DISTINCT, real PomBase ORF (SPCC16C4.20c, gene name hap2, "Ino80 complex HMG box protein Hap2"), not a typo for SPCC16C4.02c. The only place "SPCC16C4.02" appears in Tay 2013 is a GOLEM GO-enrichment classification figure legend, where it is most parsimoniously a single-digit transposition typo for the experimentally tested SPCC16C4.20c/hap2. Consistent with SPCC16C4.02c's neurochondrin/ARM-repeat domains, NCDN orthology, and curated interactors (none of which are Ino80 subunits), Tay 2013 provides no reliable experimental evidence for SPCC16C4.02c (asi1/ceo6) and no support for Ino80 complex membership of this protein. No direct biochemical activity, substrate, or definitive localization has been demonstrated for SPCC16C4.02c.
| GO Term | Evidence | Action | Reason |
|---|---|---|---|
| GO:0003674 molecular_function | ND GO_REF:0000015 | REMOVE | Summary: Root molecular function term with ND evidence indicates no specific molecular function has been experimentally determined. Based on protein-protein interaction data and orthology to neurochondrin, this protein likely has protein binding activity as a scaffolding protein, but this requires experimental validation. Reason: Root terms with ND evidence should be removed as they provide no functional information. |
| GO:0008150 biological_process | ND GO_REF:0000015 | REMOVE | Summary: Root biological process term with ND evidence indicates no specific biological process has been experimentally determined. Protein interactions suggest involvement in spindle pole body organization, cortical microtubule anchoring, and cellular aging, but these require experimental validation. Reason: Root terms with ND evidence should be removed as they provide no functional information. |
| GO:0005634 nucleus | HDA PMID:16823372 ORFeome cloning and global analysis of protein localization ... | KEEP AS NON CORE | Summary: Nuclear localization assigned from a genome-wide GFP localization screen (HDA, PMID:16823372) and retained by PomBase. The human neurochondrin ortholog is predominantly cytoplasmic, so a nuclear pool is treated as possibly transient or partial. Falcon deep research surfaced Tay et al. 2013 (PMID:23365689) and reported SPCC16C4.02c as a nuclear Ino80 chromatin-remodeler doxorubicin-resistance factor, but this does NOT apply to SPCC16C4.02c: the Ino80 membership and the deletion/epistasis experiments in that paper concern Spcc16c4.20c, which PomBase confirms is a separate real ORF (SPCC16C4.20c, gene name hap2, an Ino80 complex HMG box protein) and not a typo for SPCC16C4.02c. The sole occurrence of "SPCC16C4.02" in Tay 2013 is in a GOLEM GO-enrichment figure legend, most plausibly a transposition typo for the experimentally tested SPCC16C4.20c/hap2. Tay 2013 therefore provides no evidence for a nuclear pool of SPCC16C4.02c. The HDA localization stands on its own and is consistent only with a possible transient/partial nuclear pool of this cytoplasmic-type neurochondrin ortholog. Reason: HDA nuclear localization is retained by PomBase; it is kept as non-core because the protein is a cytoplasmic-type neurochondrin ortholog and no specific nuclear molecular function is established. The previously invoked Tay et al. 2013 "additional support" is withdrawn: that paper's Ino80/nuclear evidence concerns the distinct gene SPCC16C4.20c (hap2), not SPCC16C4.02c. Supporting Evidence: PMID:16823372 ORFeome cloning and global analysis of protein localization in the fission yeast Schizosaccharomyces pombe. |
| GO:0032153 cell division site | HDA PMID:16823372 ORFeome cloning and global analysis of protein localization ... | KEEP AS NON CORE | Summary: Cell division site localization based on high-throughput data. This annotation is plausible given the protein interacts with Sfi1 (spindle pole body component) and may have roles in cell division-related processes. However, HDA evidence alone is not definitive. Reason: Possible but not definitively established localization. May reflect transient association during cell division phases. Supporting Evidence: PMID:16823372 ORFeome cloning and global analysis of protein localization in the fission yeast Schizosaccharomyces pombe. |
| GO:0044732 mitotic spindle pole body | HDA PMID:16823372 ORFeome cloning and global analysis of protein localization ... | KEEP AS NON CORE | Summary: Spindle pole body localization based on high-throughput data. This is supported by protein-protein interaction with Sfi1, a core spindle pole body component involved in SPB duplication. The localization may be cell cycle-dependent or represent a subset of the protein population. Reason: Supported by interaction data with SPB component Sfi1, suggesting possible transient or partial association with spindle pole body structures. Supporting Evidence: PMID:16823372 ORFeome cloning and global analysis of protein localization in the fission yeast Schizosaccharomyces pombe. |
| GO:0060090 molecular adaptor activity | IEA | NEW | Summary: Proposed molecular adaptor / scaffolding activity, inferred from orthology to human neurochondrin (NCDN; HGNC:17597), the neurochondrin/ARM-repeat domain architecture, and curated binary interactions with sfi1, num1/mcp5, ecl1, pof4 and rng10. This remains an inference: falcon deep research explicitly notes that no direct biochemical activity, substrate specificity, or localization has been demonstrated for SPCC16C4.02c, so the MF is supported by domain/orthology and interaction data rather than by direct assay. Reason: This molecular function term reflects SPCC16C4.02c's role as a scaffolding protein orthologous to neurochondrin that mediates protein-protein interactions in cellular organization. Supporting Evidence: file:SCHPO/SPCC16C4.02c/SPCC16C4.02c-deep-research.md By similarity, *SPCC16C4.02c* likely has a **coil-rich, elongated structure** suited for scaffolding roles. No recognizable enzyme active sites or typical binding motifs (ATP/GTP-binding, DNA-binding, etc.) are found, reinforcing the idea that it functions as an adaptor. file:SCHPO/SPCC16C4.02c/SPCC16C4.02c-deep-research-falcon.md **What is not currently supported from retrieved evidence:** a direct biochemical activity, substrate specificity (enzyme reaction), transport substrate, or a definitive subcellular localization for SPCC16C4.02c itself. |
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Download this section (compressed HTML)Q: What is the molecular function of SPCC16C4.02c as a scaffolding protein and how does it coordinate different cellular processes?
Q: How does SPCC16C4.02c regulate cell division and what specific role does it play in spindle pole body function?
Q: What determines the protein interaction specificity of SPCC16C4.02c and how are these interactions regulated during the cell cycle?
Q: How does SPCC16C4.02c contribute to cellular aging processes and what is its relationship with aging regulators like Ecl1?
Experiment: Proteomics analysis using affinity purification and mass spectrometry to comprehensively identify SPCC16C4.02c interacting partners
Experiment: Live-cell imaging of fluorescently tagged SPCC16C4.02c to study its localization dynamics during the cell cycle
Experiment: Functional analysis using gene deletion to assess the cellular consequences of SPCC16C4.02c loss
Experiment: Structural characterization to determine the protein domains responsible for different protein-protein interactions
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Download this section (compressed HTML)📊 View Pathway Visualization Interactive pathway diagram with detailed annotations