MMGT1 (ER membrane protein complex subunit 5, EMC5; also known as membrane magnesium transporter 1 / TMEM32) is a small (131 aa) polytopic ER membrane protein with two transmembrane helices and cytoplasmic N- and C-termini. It is a constitutive subunit of the ER membrane protein complex (EMC), a conserved transmembrane-domain insertase and membrane-protein chaperone of the endoplasmic reticulum. Within the complex, EMC5 packs against the catalytic insertase subunits EMC3 and EMC6 that form the hydrophilic membrane vestibule through which substrate transmembrane domains are inserted. The EMC enables the energy-independent insertion of newly synthesized membrane proteins into the ER membrane, with a preference for transmembrane domains that are weakly hydrophobic or contain destabilizing charged or aromatic residues. It mediates post-translational insertion of tail-anchored proteins and cotranslational insertion and topogenesis of multipass membrane proteins, including setting the N-exo topology of the first transmembrane domain of G protein-coupled receptors. MMGT1 localizes to the ER membrane and is broadly expressed. Its legacy designation as a membrane magnesium transporter derives from overexpression studies of the rodent ortholog and is not supported by a defined transport mechanism for the human protein.
| GO Term | Evidence | Action | Reason |
|---|---|---|---|
| GO:0022857 transmembrane transporter activity | IBA GO_REF:0000033 | MARK AS OVER ANNOTATED | Summary: Generic transmembrane transporter activity propagated phylogenetically from the legacy "membrane magnesium transporter" family inference. The verified function of MMGT1 is as an EMC insertase subunit, not as a solute transporter. Reason: No experimental evidence that human MMGT1 transports a solute; the transporter assignment derives from a legacy family name and is superseded by the EMC insertase role. Supporting Evidence: file:human/MMGT1/MMGT1-uniprot.txt May be involved in Mg(2+) transport (By similarity) |
| GO:0005769 early endosome | IBA GO_REF:0000033 | KEEP AS NON CORE | Summary: Phylogenetic early-endosome localization, ultimately derived from the rodent ortholog. The experimentally verified compartment is the ER membrane. Reason: Possible minor localization carried over by similarity but peripheral to the core ER-membrane EMC site of action. Supporting Evidence: file:human/MMGT1/MMGT1-uniprot.txt Early endosome membrane |
| GO:0005794 Golgi apparatus | IBA GO_REF:0000033 | KEEP AS NON CORE | Summary: Phylogenetic Golgi localization derived from the rodent ortholog. The core EMC site of action is the ER membrane. Reason: Possible minor localization by similarity but peripheral to the ER-membrane EMC core function. Supporting Evidence: file:human/MMGT1/MMGT1-uniprot.txt Golgi |
| GO:0005886 plasma membrane | IBA GO_REF:0000033 | MARK AS OVER ANNOTATED | Summary: Phylogenetic plasma-membrane localization, weakly supported and most likely reflecting the transporter-family inference. MMGT1 acts in the ER membrane as an EMC subunit. Reason: Not supported by experimental evidence for the human protein; inconsistent with the ER-membrane EMC role. Supporting Evidence: file:human/MMGT1/MMGT1-uniprot.txt SUBCELLULAR LOCATION: Endoplasmic reticulum membrane |
| GO:0072546 EMC complex | IBA GO_REF:0000033 | ACCEPT | Summary: MMGT1/EMC5 is a constitutive subunit of the ER membrane protein complex; phylogenetic assignment is consistent with direct experimental and structural evidence. Core structural identity. Reason: EMC complex membership is the core cellular-component identity of MMGT1; supported by IDA, cryo-EM structures, and the conserved EMC5 family. Supporting Evidence: file:human/MMGT1/MMGT1-uniprot.txt Component of the ER membrane protein complex (EMC). |
| GO:0000139 Golgi membrane | IEA GO_REF:0000044 | KEEP AS NON CORE | Summary: Electronic transfer of a Golgi membrane location from the UniProt subcellular location vocabulary, itself a By-similarity assignment from the rodent ortholog. Reason: Possible minor localization by similarity; peripheral to the core ER-membrane EMC role. Supporting Evidence: file:human/MMGT1/MMGT1-uniprot.txt Golgi |
| GO:0005789 endoplasmic reticulum membrane | IEA GO_REF:0000044 | ACCEPT | Summary: Electronic transfer of the ER membrane subcellular location from UniProt; the correct and core compartment for MMGT1. Reason: Correct core location; redundant with experimental EXP/IDA evidence. Supporting Evidence: file:human/MMGT1/MMGT1-uniprot.txt SUBCELLULAR LOCATION: Endoplasmic reticulum membrane |
| GO:0006824 cobalt ion transport | IEA GO_REF:0000108 | MARK AS OVER ANNOTATED | Summary: Inter-ontology electronic inference of cobalt ion transport, derived ultimately from the legacy metal-transporter family assignment. No experimental support for cobalt transport by human MMGT1. Reason: Speculative metal-transport process inferred from a contested family name; not the verified EMC insertase function. Supporting Evidence: file:human/MMGT1/MMGT1-uniprot.txt May be involved in Mg(2+) transport (By similarity) |
| GO:0012505 endomembrane system | IEA GO_REF:0000117 | KEEP AS NON CORE | Summary: ARBA machine-learning assignment to the endomembrane system, a generic parent of the specific ER membrane localization. Reason: Correct but generic; the ER membrane term captures the informative localization. Supporting Evidence: file:human/MMGT1/MMGT1-uniprot.txt SUBCELLULAR LOCATION: Endoplasmic reticulum membrane |
| GO:0031901 early endosome membrane | IEA GO_REF:0000044 | KEEP AS NON CORE | Summary: Electronic transfer of early endosome membrane localization from UniProt, a By-similarity assignment from the rodent ortholog. Reason: Possible minor localization by similarity; peripheral to the ER-membrane EMC core function. Supporting Evidence: file:human/MMGT1/MMGT1-uniprot.txt Early endosome membrane |
| GO:0034755 iron ion transmembrane transport | IEA GO_REF:0000108 | MARK AS OVER ANNOTATED | Summary: Inter-ontology electronic inference of iron transmembrane transport from the legacy metal-transporter family. No experimental support for iron transport by human MMGT1. Reason: Speculative metal-transport process; not the verified EMC insertase function. Supporting Evidence: file:human/MMGT1/MMGT1-uniprot.txt May be involved in Mg(2+) transport (By similarity) |
| GO:0055085 transmembrane transport | IEA GO_REF:0000108 | MARK AS OVER ANNOTATED | Summary: Generic transmembrane transport process inferred electronically from the transporter-activity assignment. Not the verified EMC insertase function. Reason: Derived from the contested transporter-family inference; superseded by the EMC role. Supporting Evidence: file:human/MMGT1/MMGT1-uniprot.txt May be involved in Mg(2+) transport (By similarity) |
| GO:1903830 magnesium ion transmembrane transport | IEA GO_REF:0000108 | MARK AS OVER ANNOTATED | Summary: Inter-ontology electronic inference of magnesium transmembrane transport. UniProt records only a By-similarity possibility of Mg(2+) transport for human MMGT1; the consensus role is as an EMC insertase subunit. Reason: Speculative, By-similarity metal transport not demonstrated for the human protein; not a core function. Supporting Evidence: file:human/MMGT1/MMGT1-uniprot.txt May be involved in Mg(2+) transport (By similarity) |
| GO:0005515 protein binding | IPI PMID:22119785 Defining human ERAD networks through an integrative mapping ... | KEEP AS NON CORE | Summary: IntAct interactions from the foundational ERAD-network mapping study that first defined the EMC, including the EMC subunits EMC2 and EMC6. Genuine EMC partnership, but bare protein binding is uninformative. Reason: Real EMC partner interactions but the bare protein binding term is uninformative per curation guidelines. Supporting Evidence: file:human/MMGT1/MMGT1-uniprot.txt Q8N4V1; Q9BV81: EMC6 |
| GO:0005515 protein binding | IPI PMID:26496610 A human interactome in three quantitative dimensions organiz... | KEEP AS NON CORE | Summary: Quantitative interactome (stoichiometry/abundance) capture; reflects EMC and membrane-protein partnerships. Bare protein binding is uninformative. Reason: High-throughput interaction; bare protein binding is uninformative and not core. Supporting Evidence: file:human/MMGT1/MMGT1-uniprot.txt Q8N4V1; Q15006: EMC2 |
| GO:0005515 protein binding | IPI PMID:28514442 Architecture of the human interactome defines protein commun... | KEEP AS NON CORE | Summary: High-throughput interactome (BioPlex protein communities) capture. Bare protein binding is uninformative. Reason: High-throughput interaction; bare protein binding is uninformative and not core. Supporting Evidence: file:human/MMGT1/MMGT1-uniprot.txt Q8N4V1; Q15006: EMC2 |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | KEEP AS NON CORE | Summary: Binary (HuRI) interactome captures of MMGT1 with multiple membrane proteins, many plausibly EMC clients. Bare protein binding is uninformative. Reason: High-throughput interactions partly reflecting client engagement; the bare term is uninformative and not core. Supporting Evidence: file:human/MMGT1/MMGT1-uniprot.txt Q8N4V1; Q9Y3D6: FIS1 |
| GO:0005515 protein binding | IPI PMID:32439656 Structural basis for membrane insertion by the human ER memb... | KEEP AS NON CORE | Summary: Interaction evidence from the cryo-EM structural study of the human EMC, reflecting genuine intra-complex partnerships. Bare protein binding is uninformative. Reason: Real intra-complex interaction; the EMC complex membership term captures the informative content. Supporting Evidence: file:human/MMGT1/MMGT1-uniprot.txt Q8N4V1; Q9BV81: EMC6 |
| GO:0005515 protein binding | IPI PMID:33961781 Dual proteome-scale networks reveal cell-specific remodeling... | KEEP AS NON CORE | Summary: BioPlex affinity-MS interactome capture. Genuine partners but the bare term is uninformative. Reason: High-throughput interaction; bare protein binding is uninformative and not core. Supporting Evidence: file:human/MMGT1/MMGT1-uniprot.txt Q8N4V1; Q15006: EMC2 |
| GO:0005515 protein binding | IPI PMID:35271311 OpenCell: Endogenous tagging for the cartography of human ce... | KEEP AS NON CORE | Summary: OpenCell endogenous-tagging interactome capture. Bare protein binding is uninformative. Reason: High-throughput interaction; bare protein binding is uninformative and not core. Supporting Evidence: file:human/MMGT1/MMGT1-uniprot.txt Q8N4V1; Q15006: EMC2 |
| GO:0005515 protein binding | IPI PMID:40355756 The solute carrier superfamily interactome. | KEEP AS NON CORE | Summary: Solute carrier (SLC) superfamily interactome capture; many SLC partners are plausible EMC clients. Bare protein binding is uninformative. Reason: High-throughput interactions partly reflecting client engagement; the bare term is uninformative and not core. Supporting Evidence: file:human/MMGT1/MMGT1-uniprot.txt Q8N4V1; Q969S0: SLC35B4 |
| GO:0005737 cytoplasm | IEA GO_REF:0000120 | MARK AS OVER ANNOTATED | Summary: Combined-IEA assignment of cytoplasm; MMGT1 is an integral ER membrane protein. Cytoplasm is an imprecise parent relative to the experimentally supported ER membrane localization. Reason: Generic and imprecise; the specific compartment is the ER membrane. Supporting Evidence: file:human/MMGT1/MMGT1-uniprot.txt SUBCELLULAR LOCATION: Endoplasmic reticulum membrane |
| GO:0005769 early endosome | IEA GO_REF:0000107 | KEEP AS NON CORE | Summary: Ensembl-Compara electronic transfer of early endosome localization from the rodent ortholog. Reason: Possible minor localization by similarity; peripheral to the ER-membrane EMC core function. Supporting Evidence: file:human/MMGT1/MMGT1-uniprot.txt Early endosome membrane |
| GO:0005794 Golgi apparatus | IEA GO_REF:0000107 | KEEP AS NON CORE | Summary: Ensembl-Compara electronic transfer of Golgi localization from the rodent ortholog. Reason: Possible minor localization by similarity; peripheral to the ER-membrane EMC core function. Supporting Evidence: file:human/MMGT1/MMGT1-uniprot.txt Golgi |
| GO:0005886 plasma membrane | IEA GO_REF:0000107 | MARK AS OVER ANNOTATED | Summary: Ensembl-Compara electronic transfer of plasma membrane localization; weakly supported and most likely reflecting transporter-family inference. Reason: Not supported experimentally for the human protein; inconsistent with the ER-membrane EMC role. Supporting Evidence: file:human/MMGT1/MMGT1-uniprot.txt SUBCELLULAR LOCATION: Endoplasmic reticulum membrane |
| GO:0015087 cobalt ion transmembrane transporter activity | IEA GO_REF:0000107 | MARK AS OVER ANNOTATED | Summary: Ensembl-Compara transfer of a cobalt transporter activity from the rodent ortholog. No experimental support for cobalt transport by human MMGT1. Reason: Speculative metal-transporter activity from the contested family inference; superseded by the EMC role. Supporting Evidence: file:human/MMGT1/MMGT1-uniprot.txt May be involved in Mg(2+) transport (By similarity) |
| GO:0015093 ferrous iron transmembrane transporter activity | IEA GO_REF:0000107 | MARK AS OVER ANNOTATED | Summary: Ensembl-Compara transfer of a ferrous iron transporter activity from the rodent ortholog. No experimental support for iron transport by human MMGT1. Reason: Speculative metal-transporter activity; superseded by the EMC role. Supporting Evidence: file:human/MMGT1/MMGT1-uniprot.txt May be involved in Mg(2+) transport (By similarity) |
| GO:0015095 magnesium ion transmembrane transporter activity | IEA GO_REF:0000107 | MARK AS OVER ANNOTATED | Summary: Ensembl-Compara transfer of magnesium transporter activity from the rodent ortholog. UniProt records only a By-similarity possibility of Mg(2+) transport; the consensus role is as an EMC insertase subunit. Reason: Speculative, By-similarity metal transport not demonstrated for the human protein; not a core function. Supporting Evidence: file:human/MMGT1/MMGT1-uniprot.txt May be involved in Mg(2+) transport (By similarity) |
| GO:0022857 transmembrane transporter activity | IEA GO_REF:0000107 | MARK AS OVER ANNOTATED | Summary: Ensembl-Compara transfer of generic transmembrane transporter activity from the rodent ortholog. Not the verified EMC insertase function. Reason: Derived from the contested transporter-family inference; superseded by the EMC role. Supporting Evidence: file:human/MMGT1/MMGT1-uniprot.txt May be involved in Mg(2+) transport (By similarity) |
| GO:0000139 Golgi membrane | ISS GO_REF:0000024 | KEEP AS NON CORE | Summary: Curator ISS transfer of Golgi membrane localization from the rodent ortholog. Reason: Possible minor localization by similarity; peripheral to the ER-membrane EMC core function. Supporting Evidence: file:human/MMGT1/MMGT1-uniprot.txt Golgi |
| GO:0005789 endoplasmic reticulum membrane | EXP PMID:22119785 Defining human ERAD networks through an integrative mapping ... | ACCEPT | Summary: Experimental ER membrane localization from the EMC-discovery ERAD-network study. Core compartment. Reason: Experimentally supported core location. Supporting Evidence: file:human/MMGT1/MMGT1-uniprot.txt SUBCELLULAR LOCATION: Endoplasmic reticulum membrane |
| GO:0031901 early endosome membrane | ISS GO_REF:0000024 | KEEP AS NON CORE | Summary: Curator ISS transfer of early endosome membrane localization from the rodent ortholog. Reason: Possible minor localization by similarity; peripheral to the ER-membrane EMC core function. Supporting Evidence: file:human/MMGT1/MMGT1-uniprot.txt Early endosome membrane |
| GO:0005789 endoplasmic reticulum membrane | NAS PMID:29242231 The ER membrane protein complex is a transmembrane domain in... | ACCEPT | Summary: NAS annotation of ER membrane localization for the EMC from the insertase study, consistent with experimental evidence and the core compartment of MMGT1. Reason: Correct core location; consistent with EXP/IDA evidence. Supporting Evidence: file:human/MMGT1/MMGT1-uniprot.txt SUBCELLULAR LOCATION: Endoplasmic reticulum membrane |
| GO:0045050 protein insertion into ER membrane by stop-transfer membrane-anchor sequence | IDA PMID:29242231 The ER membrane protein complex is a transmembrane domain in... | ACCEPT | Summary: The EMC inserts transmembrane domains, including stop-transfer membrane-anchor sequences of multipass proteins; MMGT1/EMC5 is part of the insertase. A core biological process of the EMC. Reason: Core EMC-mediated process; MMGT1 contributes as a constitutive subunit, and EMC5 depletion reduces client insertion. Supporting Evidence: PMID:29242231 The ER membrane protein complex is a transmembrane domain insertase |
| GO:0071816 tail-anchored membrane protein insertion into ER membrane | IDA PMID:29242231 The ER membrane protein complex is a transmembrane domain in... | ACCEPT | Summary: The EMC mediates post-translational insertion of tail-anchored proteins; demonstrated directly with the reconstituted complex. A core EMC process to which MMGT1 contributes as a subunit. Reason: Core EMC-mediated process; directly demonstrated. Supporting Evidence: PMID:29242231 The ER membrane protein complex is a transmembrane domain insertase |
| GO:0072546 EMC complex | IPI PMID:32439656 Structural basis for membrane insertion by the human ER memb... | ACCEPT | Summary: ComplexPortal/structural IPI assignment of EMC complex membership based on the cryo-EM structure of the human EMC. Core structural identity of MMGT1. Reason: Structurally demonstrated core EMC membership. Supporting Evidence: file:human/MMGT1/MMGT1-uniprot.txt Component of the ER membrane protein complex (EMC). |
| GO:0032977 membrane insertase activity | IMP PMID:34918864 EMC is required for biogenesis of Xport-A, an essential chap... | KEEP AS NON CORE | Summary: In vivo Drosophila evidence that the EMC, including EMC5, is required for TMD membrane insertion of a tail-anchored client. MMGT1 is a small non-catalytic membrane subunit, so the insertase activity is a complex-level property to which it contributes. Reason: contributes_to is appropriate, but MMGT1 is not the catalytic insertase subunit (EMC3/EMC6 form the vestibule); the core MMGT1 identity is EMC membership and ER-membrane localization. Supporting Evidence: file:human/MMGT1/MMGT1-uniprot.txt energy-independent insertion into endoplasmic |
| GO:0071816 tail-anchored membrane protein insertion into ER membrane | IMP PMID:34918864 EMC is required for biogenesis of Xport-A, an essential chap... | ACCEPT | Summary: In vivo (Drosophila) IMP evidence that the EMC, including EMC5, is required for tail-anchored protein insertion. Core EMC process. Reason: Core EMC process; supported by in vivo loss-of-function. Supporting Evidence: file:human/MMGT1/MMGT1-uniprot.txt post-translational insertion of tail-anchored/TA proteins in |
| GO:0032977 membrane insertase activity | IMP PMID:29809151 The ER membrane protein complex interacts cotranslationally ... | KEEP AS NON CORE | Summary: IMP evidence that EMC subunit depletion impairs membrane insertion; MMGT1 contributes to the complex-level insertase activity but is not the catalytic subunit. Reason: contributes_to is appropriate; complex-level catalysis by EMC3/EMC6, so not MMGT1's standalone core MF. Supporting Evidence: file:human/MMGT1/MMGT1-uniprot.txt energy-independent insertion into endoplasmic |
| GO:0032977 membrane insertase activity | IMP PMID:30415835 EMC Is Required to Initiate Accurate Membrane Protein Topoge... | KEEP AS NON CORE | Summary: IMP evidence (topogenesis study) supporting the EMC's membrane insertase activity, to which MMGT1 contributes as a subunit. Reason: contributes_to is appropriate; complex-level catalysis, not MMGT1's standalone core MF. Supporting Evidence: file:human/MMGT1/MMGT1-uniprot.txt energy-independent insertion into endoplasmic |
| GO:0045050 protein insertion into ER membrane by stop-transfer membrane-anchor sequence | IMP PMID:29809151 The ER membrane protein complex interacts cotranslationally ... | ACCEPT | Summary: The EMC is required for cotranslational insertion of multipass proteins in which stop-transfer membrane-anchor sequences become membrane-spanning helices; MMGT1 is part of the insertase. Core EMC process. Reason: Core EMC-mediated process; supported by IMP of EMC subunits. Supporting Evidence: file:human/MMGT1/MMGT1-uniprot.txt stop-transfer membrane-anchor sequences become ER membrane spanning |
| GO:0005789 endoplasmic reticulum membrane | IDA PMID:32439656 Structural basis for membrane insertion by the human ER memb... | ACCEPT | Summary: Direct (structural) evidence placing MMGT1 in the ER membrane. Core compartment. Reason: Experimentally supported core location. Supporting Evidence: file:human/MMGT1/MMGT1-uniprot.txt SUBCELLULAR LOCATION: Endoplasmic reticulum membrane |
| GO:0045050 protein insertion into ER membrane by stop-transfer membrane-anchor sequence | IMP PMID:30415835 EMC Is Required to Initiate Accurate Membrane Protein Topoge... | ACCEPT | Summary: IMP (topogenesis study) supporting the EMC's role in insertion of stop-transfer membrane-anchor sequences and N-exo topogenesis of multipass clients. Core EMC process. Reason: Core EMC-mediated process. Supporting Evidence: file:human/MMGT1/MMGT1-uniprot.txt stop-transfer membrane-anchor sequences become ER membrane spanning |
| GO:0071816 tail-anchored membrane protein insertion into ER membrane | IMP PMID:29242231 The ER membrane protein complex is a transmembrane domain in... | ACCEPT | Summary: IMP evidence that the EMC is required for tail-anchored protein insertion into the ER membrane; MMGT1 is part of the insertase. Core EMC process. Reason: Core EMC-mediated process; directly demonstrated. Supporting Evidence: file:human/MMGT1/MMGT1-uniprot.txt post-translational insertion of tail-anchored/TA proteins in |
| GO:0016020 membrane | HDA PMID:19946888 Defining the membrane proteome of NK cells. | KEEP AS NON CORE | Summary: High-throughput membrane-proteome detection (NK cell membrane proteome); a generic membrane localization, a parent of the specific ER membrane term. Reason: Correct but generic; the ER membrane term captures the informative localization. Supporting Evidence: file:human/MMGT1/MMGT1-uniprot.txt SUBCELLULAR LOCATION: Endoplasmic reticulum membrane |
| GO:0016020 membrane | IDA PMID:22119785 Defining human ERAD networks through an integrative mapping ... | KEEP AS NON CORE | Summary: Direct generic membrane localization from the EMC-discovery study; a parent of the specific ER membrane term. Reason: Correct but generic; the ER membrane term captures the informative localization. Supporting Evidence: file:human/MMGT1/MMGT1-uniprot.txt SUBCELLULAR LOCATION: Endoplasmic reticulum membrane |
| GO:0072546 EMC complex | IDA PMID:22119785 Defining human ERAD networks through an integrative mapping ... | ACCEPT | Summary: Direct experimental identification of MMGT1/EMC5 in the EMC by the foundational ERAD-network mapping study. Core structural identity. Reason: Core EMC membership; directly demonstrated. Supporting Evidence: file:human/MMGT1/MMGT1-uniprot.txt Component of the ER membrane protein complex (EMC). |
| GO:0005769 early endosome | ISS GO_REF:0000024 | KEEP AS NON CORE | Summary: Curator ISS transfer of early endosome localization from the rodent ortholog. Reason: Possible minor localization by similarity; peripheral to the ER-membrane EMC core function. Supporting Evidence: file:human/MMGT1/MMGT1-uniprot.txt Early endosome membrane |
| GO:0005794 Golgi apparatus | ISS GO_REF:0000024 | KEEP AS NON CORE | Summary: Curator ISS transfer of Golgi localization from the rodent ortholog. Reason: Possible minor localization by similarity; peripheral to the ER-membrane EMC core function. Supporting Evidence: file:human/MMGT1/MMGT1-uniprot.txt Golgi |
| GO:0015095 magnesium ion transmembrane transporter activity | ISS GO_REF:0000024 | MARK AS OVER ANNOTATED | Summary: Curator ISS transfer of magnesium transporter activity from the rodent ortholog. UniProt records only a By-similarity possibility of Mg(2+) transport; the consensus role is as an EMC insertase subunit. Reason: Speculative, By-similarity metal transport not demonstrated for the human protein; not a core function. Supporting Evidence: file:human/MMGT1/MMGT1-uniprot.txt May be involved in Mg(2+) transport (By similarity) |
| GO:0015693 magnesium ion transport | ISS GO_REF:0000024 | MARK AS OVER ANNOTATED | Summary: Curator ISS transfer of a magnesium transport process from the rodent ortholog. Not demonstrated for the human protein. Reason: Speculative, By-similarity metal transport; superseded by the EMC insertase role and not a core function. Supporting Evidence: file:human/MMGT1/MMGT1-uniprot.txt May be involved in Mg(2+) transport (By similarity) |
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Download this section (compressed HTML)Q: Does human MMGT1/EMC5 have any genuine magnesium (or other metal) transport activity in its physiological ER context, or is the reported metal transport an overexpression artifact unrelated to its EMC function?
Q: What is the specific structural contribution of EMC5 to stability and substrate gating of the EMC3/EMC6 insertase vestibule?
Experiment: Reconstitute purified EMC with and without EMC5 into proteoliposomes and assay both model TMD insertion and putative Mg2+ flux, to separate any intrinsic transport activity from the insertase function.
Experiment: Quantitative membrane proteomics of EMC5-knockout versus rescued cells to define the EMC5-dependent client repertoire and test whether magnesium homeostasis phenotypes are direct or secondary to impaired client biogenesis.
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