MetaboLights MTBLS19 → GO MF enrichment
Input: MetaboLights MTBLS19
Generated by go_enrichment.py — closure-aware
hypergeometric (Fisher) over-representation of GO terms reached from the
metabolite set through Rhea + rhea2go, with is_a/part_of closure from
go-basic.obo. All inputs fetched live; nothing hardcoded.
- Foreground: 21 study metabolites mapped into Rhea-participant space
(normalized for protonation + skeleton). - Background universe: 7174 Rhea participants reaching ≥1 GO term via rhea2go.
- Test: one-sided hypergeometric per GO term; Benjamini-Hochberg FDR.
Top enriched GO terms
| GO term | Aspect | k/n | K/N | Fold | FDR |
|---|---|---|---|---|---|
| GO:0016811 hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds, in linear amides | MF | 8/21 | 354/7174 | 7.7× | 4.4e-04 |
| GO:0016810 hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds | MF | 9/21 | 513/7174 | 6.0× | 4.4e-04 |
| GO:0047747 cholate-CoA ligase activity | MF | 3/21 | 14/7174 | 73.2× | 4.4e-04 |
| GO:0016405 CoA-ligase activity | MF | 6/21 | 224/7174 | 9.2× | 9.3e-04 |
| GO:0016877 ligase activity, forming carbon-sulfur bonds | MF | 6/21 | 236/7174 | 8.7× | 9.3e-04 |
| GO:0016878 acid-thiol ligase activity | MF | 6/21 | 236/7174 | 8.7× | 9.3e-04 |
| GO:0033881 bile-acid-CoA transferase activity | MF | 2/21 | 4/7174 | 170.8× | 9.3e-04 |
| GO:0047742 chenodeoxycholoyltaurine hydrolase activity | MF | 2/21 | 4/7174 | 170.8× | 9.3e-04 |
| GO:0045302 choloylglycine hydrolase activity | MF | 2/21 | 4/7174 | 170.8× | 9.3e-04 |
| GO:0050617 15,16-dihydrobiliverdin:ferredoxin oxidoreductase activity | MF | 2/21 | 5/7174 | 136.6× | 1.3e-03 |
| GO:0017040 N-acylsphingosine amidohydrolase activity | MF | 3/21 | 30/7174 | 34.2× | 1.3e-03 |
| GO:0004090 carbonyl reductase (NADPH) activity | MF | 4/21 | 89/7174 | 15.4× | 1.6e-03 |
| GO:0016289 acyl-CoA hydrolase activity | MF | 5/21 | 175/7174 | 9.8× | 1.6e-03 |
| GO:0016790 thiolester hydrolase activity | MF | 5/21 | 196/7174 | 8.7× | 2.4e-03 |
| GO:0016874 ligase activity | MF | 7/21 | 456/7174 | 5.2× | 2.4e-03 |
| GO:0016406 carnitine O-acyltransferase activity | MF | 2/21 | 8/7174 | 85.4× | 2.4e-03 |
| GO:0008397 sterol 12-alpha-hydroxylase activity | MF | 2/21 | 11/7174 | 62.1× | 4.5e-03 |
| GO:0016712 oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, reduced flavin or flavoprotein as one donor, and incorporation of one atom of oxygen | MF | 6/21 | 374/7174 | 5.5× | 5.1e-03 |
| GO:0170055 lipid transmembrane transporter activity | MF | 2/21 | 14/7174 | 48.8× | 6.5e-03 |
| GO:0160215 deacylase activity | MF | 5/21 | 260/7174 | 6.6× | 6.5e-03 |
| GO:0120502 fatty acid omega-1 hydroxylase activity | MF | 2/21 | 17/7174 | 40.2× | 8.5e-03 |
| GO:0102672 fatty acid alpha-dioxygenase activity | MF | 2/21 | 17/7174 | 40.2× | 8.5e-03 |
| GO:0016636 oxidoreductase activity, acting on the CH-CH group of donors, iron-sulfur protein as acceptor | MF | 2/21 | 20/7174 | 34.2× | 1.1e-02 |
| GO:0008106 alcohol dehydrogenase (NADP+) activity | MF | 4/21 | 183/7174 | 7.5× | 1.3e-02 |
| GO:0016787 hydrolase activity | MF | 12/21 | 1839/7174 | 2.2× | 1.4e-02 |
| GO:0140657 ATP-dependent activity | MF | 4/21 | 220/7174 | 6.2× | 2.3e-02 |
| GO:0047617 fatty acyl-CoA hydrolase activity | MF | 3/21 | 113/7174 | 9.1× | 2.6e-02 |
| GO:0004497 monooxygenase activity | MF | 7/21 | 751/7174 | 3.2× | 2.6e-02 |
| GO:0015020 glucuronosyltransferase activity | MF | 3/21 | 119/7174 | 8.6× | 2.8e-02 |
| GO:0016788 hydrolase activity, acting on ester bonds | MF | 8/21 | 992/7174 | 2.8× | 2.9e-02 |
Reading this
k/n = foreground metabolites annotated to the term / foreground size;
K/N = same in the background universe; Fold = (k/n)/(K/N). Terms reached
via rhea2go are GO molecular function; closure lifts them to more general
function terms. BP-level lift via the enzyme/gene layer (GOA / GO-CAM) is the
documented next step (see the project page).