MetaboLights MTBLS404 → GO MF enrichment
Input: MetaboLights MTBLS404
Generated by go_enrichment.py — closure-aware
hypergeometric (Fisher) over-representation of GO terms reached from the
metabolite set through Rhea + rhea2go, with is_a/part_of closure from
go-basic.obo. All inputs fetched live; nothing hardcoded.
- Foreground: 81 study metabolites mapped into Rhea-participant space
(normalized for protonation + skeleton). - Background universe: 7174 Rhea participants reaching ≥1 GO term via rhea2go.
- Test: one-sided hypergeometric per GO term; Benjamini-Hochberg FDR.
Top enriched GO terms
| GO term | Aspect | k/n | K/N | Fold | FDR |
|---|---|---|---|---|---|
| GO:0016810 hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds | MF | 32/81 | 513/7174 | 5.5× | 1.3e-13 |
| GO:0016811 hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds, in linear amides | MF | 26/81 | 354/7174 | 6.5× | 2.4e-12 |
| GO:0016903 oxidoreductase activity, acting on the aldehyde or oxo group of donors | MF | 25/81 | 356/7174 | 6.2× | 1.3e-11 |
| GO:0160215 deacylase activity | MF | 22/81 | 260/7174 | 7.5× | 1.3e-11 |
| GO:0004030 aldehyde dehydrogenase [NAD(P)+] activity | MF | 20/81 | 231/7174 | 7.7× | 1.2e-10 |
| GO:0016874 ligase activity | MF | 26/81 | 456/7174 | 5.0× | 3.0e-10 |
| GO:0004029 aldehyde dehydrogenase (NAD+) activity | MF | 18/81 | 190/7174 | 8.4× | 3.0e-10 |
| GO:0016620 oxidoreductase activity, acting on the aldehyde or oxo group of donors, NAD or NADP as acceptor | MF | 22/81 | 313/7174 | 6.2× | 3.0e-10 |
| GO:0016787 hydrolase activity | MF | 49/81 | 1839/7174 | 2.4× | 3.7e-09 |
| GO:0016790 thiolester hydrolase activity | MF | 16/81 | 196/7174 | 7.2× | 4.3e-08 |
| GO:0047661 amino-acid racemase activity | MF | 10/81 | 57/7174 | 15.5× | 5.7e-08 |
| GO:0016289 acyl-CoA hydrolase activity | MF | 15/81 | 175/7174 | 7.6× | 6.9e-08 |
| GO:0046943 carboxylic acid transmembrane transporter activity | MF | 9/81 | 44/7174 | 18.1× | 8.8e-08 |
| GO:0015556 C4-dicarboxylate transmembrane transporter activity | MF | 6/81 | 12/7174 | 44.3× | 1.3e-07 |
| GO:0016830 carbon-carbon lyase activity | MF | 21/81 | 415/7174 | 4.5× | 2.6e-07 |
| GO:0016855 racemase and epimerase activity, acting on amino acids and derivatives | MF | 10/81 | 69/7174 | 12.8× | 2.8e-07 |
| GO:0005310 dicarboxylic acid transmembrane transporter activity | MF | 6/81 | 14/7174 | 38.0× | 3.3e-07 |
| GO:0015291 secondary active transmembrane transporter activity | MF | 9/81 | 57/7174 | 14.0× | 7.2e-07 |
| GO:0016641 oxidoreductase activity, acting on the CH-NH2 group of donors, oxygen as acceptor | MF | 12/81 | 141/7174 | 7.5× | 2.8e-06 |
| GO:0008131 primary methylamine oxidase activity | MF | 11/81 | 119/7174 | 8.2× | 4.4e-06 |
| GO:0004497 monooxygenase activity | MF | 26/81 | 751/7174 | 3.1× | 5.0e-06 |
| GO:0001716 L-amino-acid oxidase activity | MF | 8/81 | 52/7174 | 13.6× | 5.0e-06 |
| GO:0016638 oxidoreductase activity, acting on the CH-NH2 group of donors | MF | 13/81 | 183/7174 | 6.3× | 5.3e-06 |
| GO:0051213 dioxygenase activity | MF | 16/81 | 292/7174 | 4.9× | 5.7e-06 |
| GO:0016788 hydrolase activity, acting on ester bonds | MF | 30/81 | 992/7174 | 2.7× | 6.1e-06 |
| GO:0016831 carboxy-lyase activity | MF | 14/81 | 221/7174 | 5.6× | 6.1e-06 |
| GO:0016405 CoA-ligase activity | MF | 14/81 | 224/7174 | 5.5× | 6.9e-06 |
| GO:0022804 active transmembrane transporter activity | MF | 11/81 | 134/7174 | 7.3× | 1.1e-05 |
| GO:0016854 racemase and epimerase activity | MF | 12/81 | 165/7174 | 6.4× | 1.1e-05 |
| GO:0050018 amino-acid dehydrogenase [NAD(P)+] activity | MF | 7/81 | 41/7174 | 15.1× | 1.1e-05 |
Reading this
k/n = foreground metabolites annotated to the term / foreground size;
K/N = same in the background universe; Fold = (k/n)/(K/N). Terms reached
via rhea2go are GO molecular function; closure lifts them to more general
function terms. BP-level lift via the enzyme/gene layer (GOA / GO-CAM) is the
documented next step (see the project page).