MetaboLights MTBLS90 → GO MF enrichment
Input: MetaboLights MTBLS90
Generated by go_enrichment.py — closure-aware
hypergeometric (Fisher) over-representation of GO terms reached from the
metabolite set through Rhea + rhea2go, with is_a/part_of closure from
go-basic.obo. All inputs fetched live; nothing hardcoded.
- Foreground: 109 study metabolites mapped into Rhea-participant space
(normalized for protonation + skeleton). - Background universe: 7174 Rhea participants reaching ≥1 GO term via rhea2go.
- Test: one-sided hypergeometric per GO term; Benjamini-Hochberg FDR.
Top enriched GO terms
| GO term | Aspect | k/n | K/N | Fold | FDR |
|---|---|---|---|---|---|
| GO:0016298 lipase activity | MF | 32/109 | 260/7174 | 8.1× | 2.3e-18 |
| GO:0052689 carboxylic ester hydrolase activity | MF | 36/109 | 428/7174 | 5.5× | 9.5e-16 |
| GO:0016788 hydrolase activity, acting on ester bonds | MF | 51/109 | 992/7174 | 3.4× | 1.2e-14 |
| GO:0004623 A2-type glycerophospholipase activity | MF | 12/109 | 25/7174 | 31.6× | 5.5e-14 |
| GO:0016787 hydrolase activity | MF | 66/109 | 1839/7174 | 2.4× | 1.0e-12 |
| GO:0016874 ligase activity | MF | 33/109 | 456/7174 | 4.8× | 1.0e-12 |
| GO:0120569 phospholipase activity | MF | 20/109 | 155/7174 | 8.5× | 8.6e-12 |
| GO:0016405 CoA-ligase activity | MF | 23/109 | 224/7174 | 6.8× | 1.3e-11 |
| GO:0004620 glycerophospholipase activity | MF | 19/109 | 146/7174 | 8.6× | 2.5e-11 |
| GO:0160215 deacylase activity | MF | 24/109 | 260/7174 | 6.1× | 2.6e-11 |
| GO:0016878 acid-thiol ligase activity | MF | 23/109 | 236/7174 | 6.4× | 2.6e-11 |
| GO:0016877 ligase activity, forming carbon-sulfur bonds | MF | 23/109 | 236/7174 | 6.4× | 2.6e-11 |
| GO:0047661 amino-acid racemase activity | MF | 13/109 | 57/7174 | 15.0× | 7.3e-11 |
| GO:0016811 hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds, in linear amides | MF | 26/109 | 354/7174 | 4.8× | 3.9e-10 |
| GO:0016855 racemase and epimerase activity, acting on amino acids and derivatives | MF | 13/109 | 69/7174 | 12.4× | 8.5e-10 |
| GO:0005324 long-chain fatty acid transmembrane transporter activity | MF | 6/109 | 7/7174 | 56.4× | 2.6e-09 |
| GO:0015245 fatty acid transmembrane transporter activity | MF | 6/109 | 7/7174 | 56.4× | 2.6e-09 |
| GO:0016790 thiolester hydrolase activity | MF | 19/109 | 196/7174 | 6.4× | 2.6e-09 |
| GO:0016289 acyl-CoA hydrolase activity | MF | 18/109 | 175/7174 | 6.8× | 3.0e-09 |
| GO:0120515 fatty acid-CoA ligase activity | MF | 16/109 | 136/7174 | 7.7× | 4.6e-09 |
| GO:0047184 1-acylglycerophosphocholine O-acyltransferase activity | MF | 11/109 | 51/7174 | 14.2× | 4.9e-09 |
| GO:0016810 hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds | MF | 29/109 | 513/7174 | 3.7× | 7.8e-09 |
| GO:0015645 fatty acid ligase activity | MF | 16/109 | 142/7174 | 7.4× | 7.8e-09 |
| GO:0170055 lipid transmembrane transporter activity | MF | 7/109 | 14/7174 | 32.9× | 1.2e-08 |
| GO:0047372 monoacylglycerol lipase activity | MF | 10/109 | 43/7174 | 15.3× | 1.3e-08 |
| GO:0047617 fatty acyl-CoA hydrolase activity | MF | 14/109 | 113/7174 | 8.2× | 2.7e-08 |
| GO:0102672 fatty acid alpha-dioxygenase activity | MF | 7/109 | 17/7174 | 27.1× | 5.9e-08 |
| GO:0004467 long-chain fatty acid-CoA ligase activity | MF | 12/109 | 82/7174 | 9.6× | 6.0e-08 |
| GO:0052816 long-chain fatty acyl-CoA hydrolase activity | MF | 10/109 | 51/7174 | 12.9× | 6.9e-08 |
| GO:0140657 ATP-dependent activity | MF | 18/109 | 220/7174 | 5.4× | 8.2e-08 |
Reading this
k/n = foreground metabolites annotated to the term / foreground size;
K/N = same in the background universe; Fold = (k/n)/(K/N). Terms reached
via rhea2go are GO molecular function; closure lifts them to more general
function terms. BP-level lift via the enzyme/gene layer (GOA / GO-CAM) is the
documented next step (see the project page).