CALCOCO1
- UniProt: Q9P1Z2 · batch: proteostasis-batch-2026-06-06 · review status: COMPLETE (thorough)
- PN placement:
ALP|Autophagy substrate selection|Selective autophagy receptor|ERphagy;...|Golgiphagy;UPS|Ubiquitin and UBL binding|trafficking|selective autophagy|UBZ1-type ZnF. PN-node mappings: ERphagy type=mapped/propagation GO:0061709 reticulophagy; Golgiphagy type=no_mapping; UPS ancestors=no_mapping/context_only (GO:0140036). - Consistency: Consistent. Deep research, notes, and review YAML all frame CALCOCO1 as a dual-function protein: (core) soluble selective-autophagy receptor for ER (reticulophagy) and Golgi (Golgiphagy) binding ATG8/GABARAP via LIR/UDS motifs; (non-core, historical) CoCoA transcriptional coactivator. The PN ERphagy mapping aligns with the review's core function. The notes explicitly flag that the autophagy-receptor role is absent from curated GOA, matching the review's GOA set (dominated by coactivator + generic protein-binding terms).
- PN story / NEW pressure: Strong, defensible ADD pressure for the core receptor role, which is genuinely absent from GOA. GO:0061709 reticulophagy (BP, verified real) is the PN projection and is
new_to_goa— should be added. Review proposes NEW MF "reticulophagy receptor activity" / "Golgiphagy receptor activity"; however an existing MF term GO:0160247 autophagy cargo adaptor activity ("brings together a cargo, targeted for degradation via autophagy, to a phagophore"; verified real) plus GO:0038024 cargo receptor activity already capture this — so the bespoke MF terms over-reach as proposed-new. Recommend annotating existing GO:0160247 + GO:0061709 (and BP GO:0061709-Golgi analog if available) rather than minting new MF terms. - Mapping strategy: PN ERphagy=mapped/propagation GO:0061709 is correct and not too broad (reticulophagy is a specific leaf process). Golgiphagy=no_mapping is the right call given Kitta 2024 (PMID:38822137) shows CALCOCO1 is NOT the dominant Golgi receptor (YIPF3/4 are) — propagating Golgiphagy would over-reach. No mapping change needed.
- Evidence alignment: Partial overlap. PN cites the EMBO ER-phagy paper (CALCOCO1+VAP) and an ER-phagy review; the review's foundational autophagy reference is Stefely 2020 (PMID:31971854, full_text_unavailable, statement-only) plus Golgiphagy papers PMID:38822137/39871880. The specific EMBO "CALCOCO1 acts with VAMP-associated proteins to mediate ER-phagy" paper named in the PN dossier is NOT cited by PMID in the review — a gap.
- Verdict: Consistent; ADD reticulophagy is warranted. Avoid minting new MF terms — existing GO:0160247 covers the receptor MF.
Recommended edits: [YAML] Add GO:0061709 reticulophagy (BP, involved_in) to CALCOCO1 existing/core annotations — currently new_to_goa and the validated core process. [YAML] Replace proposed_new_terms "reticulophagy/Golgiphagy receptor activity" with existing GO:0160247 autophagy cargo adaptor activity (and GO:0038024 cargo receptor activity) as the molecular_function for the core receptor role. [REF] Add the EMBO J "CALCOCO1 acts with VAMP-associated proteins to mediate ER-phagy" PMID (named in PN dossier) to the review references.
- 2026-06-18 follow-up: Implemented the high-confidence YAML edits: added GO:0061709 reticulophagy and GO:0160247 autophagy cargo adaptor activity, updated the core receptor function, and removed the bespoke reticulophagy/Golgiphagy receptor NTR block. The separate EMBO J reference gap remains a follow-up.