CALR3

UniProt: Q96L12 · batch: proteostasis-batch-2026-06-07 · review status: COMPLETE
PN placement: ER proteostasis|Glycoproteostasis|N-glycosylation system|Lectin chaperone ; PN-node mapping: identical to CLGN — leaf [type] Lectin chaperone no_mapping; [group] N-glycosylation system → mapped GO:0006487 protein N-linked glycosylation (new_to_goa); class/branch unmapped.
Consistency: Consistent on identity (testis-specific calreticulin-family ER-lumen chaperone; calsperin/CRT2; client ADAM3; required for sperm fertility) across notes, YAML, and PN. Key point the YAML captures and PN does not: CALR3 does NOT bind calcium (negated GO:0005509, PMID:21590275, Stains-all) and UniProt flags it as a possibly lectin-independent chaperone — so the "Lectin chaperone" PN label is itself partly inaccurate for CALR3. Same GO:0006487 term-mismatch as CLGN.
PN story / NEW pressure: PN's GO:0006487 projection over-reaches: CALR3 is a folding chaperone (binds clients), not an N-glycan-transfer enzyme, and is reported lectin-INdependent for ADAM3. The review's GO:0044183 protein folding chaperone, GO:0006457, GO:0007283 spermatogenesis, GO:0005788 ER lumen, and the NOT GO:0005509 capture the biology. GO:0006487 verified real but wrong-process → do not ADD.
Mapping strategy: Same TOMM20-style over-reach as CLGN — the N-glycosylation system group projects a biosynthetic term onto a chaperone. Additionally, the Lectin chaperone leaf label conflicts with CALR3's documented lectin-independent mode, so even a lectin-specific mapping would be shaky. Recommend not propagating GO:0006487 to CALR3 and revisiting the leaf classification.
Evidence alignment: PN listed no reference titles for this row. Review anchors on PMID:21590275 (ER-lumen localization + no Ca2+ binding) and UniProt; PMID:12384296 (identification, uncached) noted. No evidence conflict; the divergence is term/label choice.
Verdict: Identity consistent, but PN GO:0006487 projection over-reaches (chaperone, not glycosyltransferase) and the Lectin chaperone label sits awkwardly with CALR3's lectin-independent, non-Ca2+ profile.

Recommended edits: [MAP] Do not propagate GO:0006487 to CALR3; remap N-glycosylation system group to a folding/glycoprotein-QC term or leave chaperone members unmapped. [MAP] Reconsider the Lectin chaperone leaf for CALR3 (documented lectin-independent). [YAML] No glycosylation annotation for CALR3.