CNPY3

UniProt: Q9BT09 · batch: proteostasis-batch-2026-06-07 · review status: COMPLETE
PN placement: ER proteostasis|Chaperone|HSP90 system|HSP90 cochaperone|Folding of TLRs ; PN-node mapping: leaf [subtype] Folding of TLRs no_mapping; [type] HSP90 cochaperone → mapped GO:0051879 Hsp90 protein binding (more_specific_than_existing_goa); group/class/branch unmapped.
Consistency: Strongly consistent. Notes, YAML, and PN all describe CNPY3 (PRAT4A) as an ER-luminal TLR-specific co-chaperone for HSP90B1/gp96 (ATP-sensitive interaction), required for TLR1/2/4/5/7/9 folding/ER exit (not TLR3), causal in DEE60. GO:0051879 verified real. The review independently proposes GO:0051879 (action NEW) for the gp96 interaction — directly matching the PN projection.
PN story / NEW pressure: PN asserts Hsp90 binding (GO:0051879) — genuinely absent from GOA (the GOA file has NO Hsp90/Hsp90-binding row, only signaling receptor binding + bare protein binding + ER lumen). So the dossier's more_specific_than_existing_goa label is technically too weak: it is effectively new_to_goa. The review goes further and proposes a coherent NEW set (GO:0051082 unfolded protein binding, GO:0051879, GO:0034975 protein folding in ER, GO:0045087 innate immune response, GO:0034123 positive regulation of TLR signaling) — all defensible and well beyond the single PN term. ADD GO:0051879 confirmed.
Mapping strategy: PN maps conservatively at [type] (cochaperone), leaving the "Folding of TLRs" leaf and HSP90-system containers unmapped — appropriate. Only refinement: the goa_status should be new_to_goa (no Hsp90-binding annotation exists in GOA), not more_specific_than_existing_goa.
Evidence alignment: PN listed no reference titles for this row. Review evidence is UniProt (gp96/TLR interactions) + Reactome (R-HSA-1678923/1678944 TLR folding by GP96/CNPY3) + mouse PRAT4A mechanism (PMID:20865800, cited in notes); GOA IPI rows (SLITRK4 etc.) correctly dismissed as HT noise. No conflict.
Verdict: Consistent; PN GO:0051879 is correct and already in the review's NEW set, but its goa_status should be new_to_goa (not "more_specific"). Review's broader NEW terms are well supported.

Recommended edits: [MAP] Correct goa_status for GO:0051879 on the CNPY3 row from more_specific_than_existing_goa to new_to_goa (no Hsp90-binding annotation exists in CNPY3 GOA). (Review already proposes GO:0051879 + the full chaperone/TLR NEW set; no YAML change needed.)