PN placement: two rows — Translation|Cytosolic translation|Ribosome-associated QC|Ubiquitination (TR) and UPS|E3 ubiquitin and UBL ligases|RING|Makorin|C3H1-type ZnF (UPS). PN-node mapping: identical to MKRN1 — RQC type→GO:0016567 (mapped); RQC group→GO:0006515 (mapped); RING group→GO:0061630 (mapped); subtype + UPS branch = no_mapping.
Consistency: Partial mismatch. The UPS/E3-ligase placement (GO:0061630, GO:0016567) matches the review perfectly — both already in GOA (IBA/IEA/ISS). BUT the PN puts MKRN2 in the same RQC node as MKRN1, projecting GO:0006515 (protein QC). The review finds no direct or inferred RQC evidence for human MKRN2 — RQC is raised only as a suggested question ("does MKRN2 contribute to co-translational QC… how is it partitioned from MKRN1?"). MKRN2's characterized biology is RELA/p65 / NF-kB (mostly ISS/by-similarity from rodent), not poly(A) RQC. So the PN RQC placement for MKRN2 is paralogy-driven over-reach, inherited from MKRN1.
PN story / NEW pressure: No defensible NEW pressure. There is no human-experimental basis to annotate MKRN2 to GO:0006515 or GO:1990116; doing so would be PARALOG_OVERANNOTATION (MKRN1 evidence applied to MKRN2). The review correctly withholds an RQC annotation. The genuine MKRN2 functions (E3 ligase, NF-kB regulation) are already captured/over-annotation-flagged.
Mapping strategy: This gene should NOT inherit the RQC-group GO:0006515 projection. The node mapping is fine as a bucket for MKRN1, but MKRN2's membership in that node is the problem — its projected GO:0006515 is unsupported for this paralog. Flag: MKRN2 RQC placement is broader/unsupported relative to the review (which has zero RQC terms).
Evidence alignment: PN row 2 cites "19489725 / rev" (makorin review, same as MKRN1). Review anchors only on RNA-binding screen PMID:22681889 (HDA, VERIFIED) plus uniprot/ISS; notably no dedicated experimental paper establishes human MKRN2's E3 activity or any RQC role — consistent with the review's caution. Divergence: PN asserts RQC; review evidence does not.
Verdict: Over-reach on the RQC branch (paralog inheritance from MKRN1). E3-ligase placement consistent. Recommended edits: [MAP] do not project GO:0006515 / GO:1990116 onto MKRN2 from the RQC node — no human-experimental RQC evidence; flag MKRN2's RQC-node membership as paralogy-driven (PARALOG_OVERANNOTATION) pending the suggested iCLIP/ribosome-profiling experiment.