PN placement:ALP|Autophagosome closure maturation and lysosome fusion|Sealing of autophagophore membrane|Localization of the ESCRT-III complex ; PN-node mapping: leaf no_mapping (members mix endosomal-sorting + SNARE genes); "Sealing" group mapped / ok_for_propagation / GO:0000045 autophagosome assembly; class context_onlyGO:0016236. Projected: GO:0000045 (more_specific_than_existing_goa).
Consistency: Largely consistent. The PN note (ALIX/ATG12-ATG3 recruits ESCRT-III for autophagosome closure) aligns with the review, which ACCEPTs GO:0016236 macroautophagy (ISS) on the strength of PMID:25686249 (ALIX required for basal autophagic flux). The autophagy-closure framing is supported. The leaf being no_mapping (heterogeneous members) is consistent with the review not asserting an autophagy-specific ESCRT-III-localization MF for ALIX.
PN story / NEW pressure: PN projects GO:0000045 autophagosome assembly (more_specific_than_existing_goa — GOA/review have the broader GO:0016236 macroautophagy). For ALIX this is more defensible than for the MVB12 subunits because the gene-specific basal-autophagy evidence (PMID:25686249) exists, but the cited support is for basal autophagic flux/late-endosome function rather than phagophore sealing specifically. Conclusion: GO:0000045 is a plausible candidate (not pure over-reach), but the review's macroautophagy ACCEPT already captures the role; adding autophagosome assembly should be expert-gated, not auto-propagated.
Mapping strategy: Node scoping is sound. The no_mapping leaf correctly avoids over-claiming; the "Sealing" group projecting GO:0000045 is acceptable as candidate for ALIX given basal-autophagy evidence, but should remain more_specific candidate rather than displacing the curated macroautophagy call.
Evidence alignment: Shared: PMID:25686249 (ATG12-ATG3–ALIX) is a PN reference and the review's macroautophagy anchor. PN also cites closure-regulator and exosome/autophagy reviews; review independently supports MVB/exosome/cytokinesis (PMID:12860994, PMID:22660413, PMID:18641129). Good overlap on the autophagy link.
Verdict: Consistent; PN autophagosome-assembly projection is a reasonable candidate but the curated macroautophagy ACCEPT already covers it. No edits required.