Focused function hypothesis
Hypothesis: The horse protein A0A9L0SKW1 localizes to the extracellular matrix.
Target: Equus caballus (NCBITaxon:9796), UniProt A0A9L0SKW1. Gene label: OLFML2A; verify identity independently rather than treating the label as proof.
Target GO claim: GO:0031012 — extracellular matrix. Verify its definition and scope.
Decisive question
Determine whether the exact sequence and credible transcript model support entry into the secretory pathway and extracellular-matrix residence. Distinguish secretion from matrix association, and distinguish the supplied protein from alternative products of the locus.
Identity and sequence inputs
- Target record: https://www.uniprot.org/uniprotkb/A0A9L0SKW1/entry
- Human comparison lead: https://www.uniprot.org/uniprotkb/Q68BL7/entry (OLFML2A). Establish the relevant orthology/isoform relationship rather than assuming it.
- Frozen current UniProt sequence: 599 residues; SHA-256
069f390f83475c7f4749a7dff36c9d553c6334ea6dc0d2004ab814d07ef297ab. - These are current sequences downloaded for the cohort on 2026-09-08. Identity with the original prediction-time input has not been established. Evaluate the supplied sequence explicitly; document any different sequence used.
>A0A9L0SKW1 Equus caballus OLFML2A
MDSQVFGDMDQVRMTSEGSDCRCKCIMRPLSKDACSRVRSGRARVEDFYTVETVSSGTDC
RCSCTAPPSSLNPCENEWKMEKLKKQAPELLKLQSMVASQMNTLEESIKANLSRENEVVR
ESMRHFSEQLKHYENHSAIMMSIKKELSSLGLQLLQKDAATAPAAGPATGPGSKAQDTAG
GKGKDTNKYGSMQKSFVDRGLPKAPKEKLLKVEKLRKEGSKSRFPQPTGKPRALAQQQAV
VRGITYYKAGRKEATEAVADNALKGTSWLEQLPPRVEGRPSEPNSAEHDEARPRTSEGVD
LAPGTPASDPTPTPTPTTTTSPMSTEPPSRPEVPSQGREASCEGTLRAVDPPVRHHSYGR
HEGAWMKDPAARDDRIYVTNYYYGNSLVEFRNLENFKQGRWSNMYKLPYNWIGTGHVVYQ
GAFYYNRAFTKNIIKYDLRQRFVASWALLPDVVYEDTTPWKWRGHSDIDFAVDESGLWVI
YPAVDDRDEAQPEVIVLSRLDPGDLSVHRETTWKTRLRRNSYGNCFLVCGILYAVDTYNQ
REGQVAYAFDTHTGTDARPQLPFLNEHAYTTQIDYNPKERVLYAWDNGHQLTYTLHFVV
Evidence and deliverable
Use primary literature and public sequence, structural and genomic resources. Select analyses that answer the decisive question; this is not a general gene review. Assess support and contrary evidence, and allow an unresolved outcome. Distinguish directly observed horse evidence, justified mammalian transfer, and results for a different protein model.
Do not consult the ai-gene-review repository's existing judgments, research syntheses or local bioinformatics analyses. Those are held out for comparison. Do not use agreement with ARBA or another prediction as biological validation. Preserve reproducible methods, accessions/versions, actual computation outputs and primary-source URLs/DOIs/PMIDs. Report the decisive findings and limitations, not just a verdict.