Focused function hypothesis
Hypothesis: The horse protein A0A9L0RGD6 positively regulates double-strand break repair via nonhomologous end joining.
Target: Equus caballus (NCBITaxon:9796), UniProt A0A9L0RGD6. Gene label: SHLD2; verify identity independently rather than treating the label as proof.
Target GO claim: GO:2001034 — positive regulation of double-strand break repair via nonhomologous end joining. Verify its definition and scope.
Decisive question
Evaluate whether the exact protein has the molecular apparatus needed for this repair role. Compare experimentally characterized mammalian isoforms and structures. Distinguish complex recruitment from execution of the repair-supporting mechanism.
Identity and sequence inputs
- Target record: https://www.uniprot.org/uniprotkb/A0A9L0RGD6/entry
- Human comparison lead: https://www.uniprot.org/uniprotkb/Q86V20/entry (SHLD2). Establish the relevant orthology/isoform relationship rather than assuming it.
- Frozen current UniProt sequence: 883 residues; SHA-256
23a8bbd5f9a69c949ff70deeaae91430af52e92bb435042251f70404110d2f95. - These are current sequences downloaded for the cohort on 2026-09-08. Identity with the original prediction-time input has not been established. Evaluate the supplied sequence explicitly; document any different sequence used.
>A0A9L0RGD6 Equus caballus SHLD2
MSGGSQVHIFWGAPIGPLKMTVSQEPTSLVSTTDPWKKIQLLYNQHSLHLKDDKCKHKNL
EDCQVLDAAGPSDLLNGHFLANSVNRSAHVKDDFVHCISETETTKSHKIPPLEMSGIPNS
DVQICGFKGRVQHLTEEEKCQQLFSENKKIADEQHKDQSNICGQNFPKNSLHLDPKCAAI
LDLVCGTEQINIGPGAAETKRVPTGHRERQTQRLEFFPSSTVDEPRSERAARKDSALNIS
TDTEFLSVMTSSQVAFLAQRKYKGQNSVNKGIVNMEIEPKASHGEMRKREDNLIKPNDGF
AEGSESGQTEAYSLELFSPVCPETESSNIRINSDKGLEENTGSQELFTFENKLLPDEICI
ESCSSGILCSQGNTFLKSSGKRNRTSEDKLGHSKALSKVLQESKKMKLVSNARDPPVEMG
QRNVSKFHGVKKTSLIKNCGSKSQKYNCLVMVLSPCHVKEINIKSGPNSGSKVPLATITV
IDQSEVKKKVFLWRTAAFWAFTVFLGDIVLLTDVTIHDDHWVGERVLQSTFTSQLLNLGS
YSSVQPEEYSSMVSDVVLQDLLAYVSSKHSYLKDLPQRRPQKMNSIEFVELACLRPDILV
HAVVRVVDITVLTEAVYSYRGQKQRKVMLTVEQTQGQHYVLVLWGPGAAWYPQLQRKKDY
IWEFKYLFVQRNCVLENLELHTTPWSSCESLFDDDIRAVTFKAKFQKSTSSFVKMSDLAV
HLEDKCSGVILIKAQILELVFPTTAAQKIALNARSSLKSIFSSLPNIVYTGCAKCGLELE
TDENRIYRQCFSCLPFTMKKIYYSAPRRGHLWHGRRRLAPRLAGGRRGPLCGEGAEPLPA
GRKQLPPATGFLAPRLLPRQCEPASQAFPETRGKNCRRFEDNP
Evidence and deliverable
Use primary literature and public sequence, structural and genomic resources. Select analyses that answer the decisive question; this is not a general gene review. Assess support and contrary evidence, and allow an unresolved outcome. Distinguish directly observed horse evidence, justified mammalian transfer, and results for a different protein model.
Do not consult the ai-gene-review repository's existing judgments, research syntheses or local bioinformatics analyses. Those are held out for comparison. Do not use agreement with ARBA or another prediction as biological validation. Preserve reproducible methods, accessions/versions, actual computation outputs and primary-source URLs/DOIs/PMIDs. Report the decisive findings and limitations, not just a verdict.