Focused function hypothesis
Hypothesis: The horse protein A0A9L0T4W6 participates in protein dephosphorylation.
Target: Equus caballus (NCBITaxon:9796), UniProt A0A9L0T4W6. Gene label: PTPRN2; verify identity independently rather than treating the label as proof.
Target GO claim: GO:0006470 — protein dephosphorylation. Verify its definition and scope.
Decisive question
Identify a direct or regulatory mechanism for participation in protein dephosphorylation. Separate phosphoprotein substrates from phosphoinositide substrates, and establish whether characterized mammalian mechanisms transfer to this protein. Lack of intrinsic catalysis alone does not exclude participation.
Identity and sequence inputs
- Target record: https://www.uniprot.org/uniprotkb/A0A9L0T4W6/entry
- Human comparison lead: https://www.uniprot.org/uniprotkb/Q92932/entry (PTPRN2). Establish the relevant orthology/isoform relationship rather than assuming it.
- Frozen current UniProt sequence: 976 residues; SHA-256
6e469207454cac638b007421577b3c8140a28f2f24ed1e0ee900486ad5d7c821. - These are current sequences downloaded for the cohort on 2026-09-08. Identity with the original prediction-time input has not been established. Evaluate the supplied sequence explicitly; document any different sequence used.
>A0A9L0T4W6 Equus caballus PTPRN2
MGPPLALLLLLLLLPRGPPAAPATRARPLPGLLGCLFEDGLCRPSETCVDGFTWQDDYTQ
HVMAQELSNLPKTYPQHPESSNPARTSMQSVDNEKRYSQEGDIALAKALQRYLPYLEALS
QATTSNLLPRMKHGRPPSEGEDALAESVLTYVAQTSALTYAPMPREDYPAGHPLRTLSRL
QPDELSPKVVGSVDRQNLVAALGAYAAQKHPPPPREGDPGLHNLLHAPWREPRVLSAPAT
PQKWPLSPGDPKHPLGRGDEALIQSLLKDLRRHQVDMASLSPLDPEEMARVIANAMQGVG
TEGEREEAGMGVGGEPGEQVDSPEAGLHEARKVGDVRDNRVQDDDDRVYEEVNRLSLTLG
DLLQDPGSQFLPGAPPLVESFKTEIKKSEDPGASLSSEEESAGVENVRSQTYVKELLERP
QVDGFGEFQRQIPGAWKEDRRLEAGAQERSGEGLELEVQPSEESGYIVTETDPLSLEKGK
ELLAGVARLLEVPMSVFVDIDVVGPAVTFKVSANVLNVTTAEAVEAAVENKDNLEKTSGL
KILQTGVGSKSKLKLRPHQAEQEDSTKFIVLTLISVAAILGVLLASGVIYCLRHSSHYRL
KEKLSGPGGHAGLDATAYQELCRQRMAVRTSDRPEAPHTSRISSVSSQFSDGPMPSPSAR
SSTSSWSEEPAQPNMDISTGHMVLAYMEDHLKNKNRLEKEWEALCAYQAEPSSSLVAQRE
ENVPKNRCPAVLTYDHSRILLKSENSHSNSDYINASPIMDHDPRNPAYIATQGPLPATVA
DFWQMVWESGCVVIVMLTPLSENGVRQCYHYWPDEGSNLYHIYEVNLVSEHIWCEDFLVR
SFYLKNLQTNETRTVTQFHFLSWYDQGVPSSTRSLLDFRRKVNKCYRGRSCPIIVHCSDG
AGRSGTYVLIDMVLNKMAKGSTVITSHRGARTCTWGGTQLGFFESMLPVRGRHCRRSHTG
WKTIRVLCTSRSLLGS
Evidence and deliverable
Use primary literature and public sequence, structural and genomic resources. Select analyses that answer the decisive question; this is not a general gene review. Assess support and contrary evidence, and allow an unresolved outcome. Distinguish directly observed horse evidence, justified mammalian transfer, and results for a different protein model.
Do not consult the ai-gene-review repository's existing judgments, research syntheses or local bioinformatics analyses. Those are held out for comparison. Do not use agreement with ARBA or another prediction as biological validation. Preserve reproducible methods, accessions/versions, actual computation outputs and primary-source URLs/DOIs/PMIDs. Report the decisive findings and limitations, not just a verdict.