Focused function hypothesis
Hypothesis: The horse protein F6TY09 participates in female pronucleus assembly.
Target: Equus caballus (NCBITaxon:9796), UniProt F6TY09. Gene label: WEE1; verify identity independently rather than treating the label as proof.
Target GO claim: GO:0035038 — female pronucleus assembly. Verify its definition and scope.
Decisive question
Resolve the relevant mammalian paralogs and original oocyte perturbation experiments, then assess transfer to horse. Distinguish participation from necessity or sufficiency and evaluate plausible compensation. Identify any direct horse expression or functional evidence.
Identity and sequence inputs
- Target record: https://www.uniprot.org/uniprotkb/F6TY09/entry
- Human comparison lead: https://www.uniprot.org/uniprotkb/P30291/entry (WEE1). Establish the relevant orthology/isoform relationship rather than assuming it.
- Frozen current UniProt sequence: 646 residues; SHA-256
f46f1c6be9cdfa7025987fbd2b3d5f5a49a4e7cbb69d75d9101e8b34234429d4. - These are current sequences downloaded for the cohort on 2026-09-08. Identity with the original prediction-time input has not been established. Evaluate the supplied sequence explicitly; document any different sequence used.
>F6TY09 Equus caballus WEE1
MSFLSRQQPPPPRRAAASCSLRQKLIFSPCSDCEEEEEEEEEEGSGHSTGEDSAFQEPDS
PLPPARSPTEPGPERRRSPGPAPGSPGELEEDMLLRGACTGADAAGGGAEGDSWEEEGFG
SSSPVKSPAAAYFLASCFSPVRCGGPGDASPRGYGARGAAEGPCSPLPDQPGTPPHKTFR
KLRLFDTPHTPKSLLSKARGIDSSSVKLRSGSLFMDTEKSGKRELDMRQTPQVNINPFTP
DSVLFHSSGQCRRRKRTYWNDSCGEDMEASDYEFEDETRPAKRITITESNMKSRYTTEFH
ELEKIGSGEFGSVFKCVKRLDGCIYAIKRSKKPLAGSVDEQNALREVYAHAVLGQHSHVV
RYFSAWAEDDHMLIQNEYCNGGSLADAISENYRRMSYFTEVDLKDLLLQVGRGLRYIHSM
SLVHMDIKPSNIFISRTSIPNAASEEGDEDDWASNKVMFKIGDLGHVTRISSPQVEEGDS
RFLANEVLQENYTHLPKADIFALALTVVCAAGAEPLPRNGDEWHEIRQGRLPRIPQVLSQ
EFTELLKVMIHPDPERRPSAMALVKHSVLLSASRKSAEQLRIELNAEKFKNSLLQKELKK
AQMAKAAAEERALFTDRMATRSTTQSNRTARLIGKKMNRSVSLTIY
Evidence and deliverable
Use primary literature and public sequence, structural and genomic resources. Select analyses that answer the decisive question; this is not a general gene review. Assess support and contrary evidence, and allow an unresolved outcome. Distinguish directly observed horse evidence, justified mammalian transfer, and results for a different protein model.
Do not consult the ai-gene-review repository's existing judgments, research syntheses or local bioinformatics analyses. Those are held out for comparison. Do not use agreement with ARBA or another prediction as biological validation. Preserve reproducible methods, accessions/versions, actual computation outputs and primary-source URLs/DOIs/PMIDs. Report the decisive findings and limitations, not just a verdict.