ARBA Rule Reviews Index

76 rule reviews. Includes reviews in progress; status and decisions reflect the source YAML.

Condition-set counts describe the sets recorded in each review, which may omit the full rule.

Rule IDDescriptionGO TermsStatusActionParsimonyLiteratureRecorded condition setsConfidence
ARBA00000900
YAML
A mega-rule with 298 condition sets attempting to annotate all E3 ubiquitin-protein ligases with EC 2.3.2.27 (ubiquitin-protein transferase activity) across bacteria, plants, animals, and viruses. The rule covers vastly different catalytic mechanisms (RING, HECT, U-box), domain architectures, and protein families.
GO:0004842 ubiquitin-protein transferase activity
COMPLETE DEPRECATE OVERLY_COMPLEX MODERATE 0 0.10
ARBA00001096
YAML
Predicts glucose-6-phosphate 1-epimerase activity (EC 5.1.3.15) based on the presence of three hierarchical InterPro domains using restrictive AND logic COMPLETE MODIFY REDUNDANT MODERATE 1 0.80
ARBA00001712
YAML
Predicts aldose 1-epimerase activity (galactose mutarotase, EC 5.1.3.3) for proteins containing aldose epimerase domains with taxonomic restrictions to Metazoa (condition set 1) or specific CATH FunFam in Eukaryota (condition set 2) COMPLETE MODIFY REDUNDANT STRONG 2 0.70
ARBA00004173
YAML
Aggregated ARBA localization rule that asserts the UniProt SUBCELLULAR LOCATION "Mitochondrion" (SL-0173) for eukaryotic proteins matching any of 1,490 OR-ed condition sets built from CATH FunFam, InterPro and PANTHER signatures. Via the UniProtKB-SubCell -> GO mapping (GO_REF:0000044) the assertion is propagated to GO:0005739 (mitochondrion) with evidence IEA / ECO:0007322. The rule currently annotates 536,854 unreviewed (TrEMBL) proteins and 0 reviewed proteins. It is not a single-family rule but an umbrella aggregating hundreds of independent branch-level classifiers of very uneven quality, ranging from canonical mitochondrial families (MIC60, TOM40, TIM/Tim10-like, cytochrome c oxidase subunits, sideroflexins) to bare single unlabeled FunFams and clade-overfitted singletons.
GO:0005739 mitochondrion
COMPLETE MODIFY OVERLY_COMPLEX MODERATE 12 0.35
ARBA00004218
YAML
A highly problematic rule that assigns acrosome subcellular localization to 35+ unrelated protein families, including epithelial sodium channels, mitochondrial enzymes, complement proteins, and other non-sperm proteins alongside legitimate sperm components COMPLETE REMOVE OVERLY_COMPLEX CONTRADICTED 0 0.10
ARBA00004725
YAML
Rule annotating proteins involved in the de novo pyrimidine biosynthesis pathway with pathway comment "Pyrimidine metabolism; UMP biosynthesis via de novo pathway". Contains 27 condition sets covering all major enzymes: carbamoyl-phosphate synthase, aspartate carbamoyltransferase, dihydroorotase, dihydroorotate dehydrogenase, orotate phosphoribosyltransferase, and orotidine 5-phosphate decarboxylase. COMPLETE MODIFY OVERLY_COMPLEX STRONG 0 0.40
ARBA00004841
YAML
TODO: Provide a concise description of what this rule predicts and how IN_PROGRESS MODIFY REDUNDANT STRONG 3 0.30
ARBA00005098
YAML
Rule targeting ureohydrolase family proteins (arginase and agmatinase) involved in nitrogen metabolism and urea cycle, but critically lacking GO term annotations COMPLETE DEPRECATE OVERLY_COMPLEX WEAK 8 0.90
ARBA00022438
YAML
A complex mega-rule with 86 condition sets attempting to annotate all aminopeptidases across multiple mechanistic families (M1, M24, M2, M49, S33) with only a keyword annotation. The rule spans diverse catalytic mechanisms (zinc-dependent, iron-dependent, serine proteases) and biological functions (protein degradation, methionine removal, hormone processing) without appropriate GO term annotations. COMPLETE MODIFY OVERLY_COMPLEX MODERATE 0 0.20
ARBA00022487
YAML
Mega-rule predicting serine esterase activity (KW-0719) across 62 highly diverse condition sets covering numerous esterase, lipase, cutinase, and hydrolase families from bacteria to mammals COMPLETE REMOVE OVERLY_COMPLEX CONTRADICTED 0 0.10
ARBA00022603
YAML
ARBA rule ARBA00022603 - Comprehensive review pending rule data access. This rule requires fetching from UniProt ARBA database to determine specific condition sets and GO annotations.
GO:UNKNOWN TODO: Fetch actual GO annotation from UniProt
IN_PROGRESS UNDECIDED UNDECIDED UNDECIDED 1 0.00
ARBA00022670
YAML
A catastrophically over-broad rule that annotates proteins with a generic "Protease" keyword if they contain ANY of 502 different protease-related InterPro domains. The rule uses a single massive OR-logic condition set that treats highly specific peptidases (e.g., signal peptide peptidases) the same as broad families (e.g., proteasome subunits), fundamentally violating principles of specificity in functional annotation. COMPLETE REMOVE OVERLY_COMPLEX CONTRADICTED 1 0.05
ARBA00022679
YAML
Catastrophically broad rule applying the "Transferase" keyword (KW-0808) to over 14 million proteins using 4,473 condition sets encompassing 1,932 unique InterPro domains and 3,730 CATH FunFam families. This represents the most extreme case of over-annotation in the ARBA system, conflating virtually all transferase activities under a single meaningless keyword. COMPLETE REMOVE OVERLY_COMPLEX CONTRADICTED 1 0.95
ARBA00022722
YAML
Highly problematic rule with 532 condition sets using 371 unique InterPro domains to assign only a vague "Nuclease" keyword annotation with no GO terms. This represents severe over-engineering and over-annotation of nuclease function. COMPLETE REMOVE OVERLY_COMPLEX WEAK 0 0.95
ARBA00022759
YAML
An extremely complex rule with 318 condition sets that all predict the same broad "Endonuclease" keyword annotation (KW-0255). This rule attempts to capture all types of nucleic acid cleaving enzymes through diverse combinations of InterPro domains, PANTHER families, and taxonomic restrictions, representing a clear example of rule over-engineering that conflicts with annotation best practices. COMPLETE REMOVE OVERLY_COMPLEX CONTRADICTED 3 0.05
ARBA00022777
YAML
Overly broad rule attempting to annotate all kinase proteins across all domains of life using 1,358 condition sets with only a keyword annotation COMPLETE REMOVE OVERLY_COMPLEX CONTRADICTED 0 0.95
ARBA00022801
YAML
ARBA rule predicting hydrolase activity based on 4070 condition sets - exceptionally complex rule requiring removal COMPLETE REMOVE OVERLY_COMPLEX CONTRADICTED 4 0.95
ARBA00022806
YAML
Assigns helicase keyword (KW-0347) to proteins containing helicase-related domains from multiple superfamilies including DEAD-box RNA helicases, DNA helicases, and other nucleic acid unwinding enzymes COMPLETE MODIFY OVERLY_COMPLEX STRONG 0 0.75
ARBA00022825
YAML
Overly complex rule with 240 condition sets attempting to capture serine protease function, but provides only keyword annotations without GO terms COMPLETE REMOVE OVERLY_COMPLEX STRONG 0 0.95
ARBA00022912
YAML
An extremely complex mega-rule with 210 condition sets attempting to annotate all protein phosphatases across multiple mechanistic families (PTPs, PPPs, PP2C, DUSPs) with only keyword annotation. The rule spans fundamentally different catalytic mechanisms (metal-dependent, cysteine-based, dual-specificity) and lacks essential GO term annotations for enzyme function. COMPLETE MODIFY OVERLY_COMPLEX MODERATE 0 0.90
ARBA00023002
YAML
Extremely large rule with 2105 condition sets combining 975 InterPro domains and 1489 CATH FunFam families to annotate oxidoreductase proteins across all domains of life COMPLETE MODIFY OVERLY_COMPLEX MODERATE 0 0.30
ARBA00023137
YAML
Rule that applies UniProt keyword "Tyrosine-protein kinase" to proteins with various kinase-related InterPro domains, CATH FunFam families, and PANTHER families across diverse taxonomic groups. Contains 98 condition sets but provides no GO annotations. COMPLETE DEPRECATE OVERLY_COMPLEX WEAK 0 0.95
ARBA00023239
YAML
Highly complex ARBA rule with 894 condition sets for detecting proteins with lyase activity based on InterPro domains, PANTHER families, and CATH FunFams across diverse taxonomic groups. Rule only assigns keyword annotation "Lyase" (KW-0456), not GO terms. Rule exceeds analysis limits due to excessive complexity, making quantitative validation impossible. COMPLETE DEPRECATE OVERLY_COMPLEX WEAK 0 0.98
ARBA00026249
YAML
Rule predicting thioredoxin-disulfide reductase (NADPH) activity based on InterPro domains and CATH FunFam classifications. The rule uses three alternative condition sets targeting the same enzymatic function.
GO:0004791 thioredoxin-disulfide reductase (NADPH) activity
COMPLETE DEPRECATE REDUNDANT STRONG 3 0.92
ARBA00026302
YAML
Massively overcomplex rule with 185 condition sets attempting to predict the broad biological process term "carbohydrate derivative biosynthetic process" (GO:1901137). The rule encompasses an extraordinarily diverse array of InterPro domains, CATH FunFams, and PANTHER families spanning multiple taxonomic groups, creating a rule of unprecedented complexity that violates all principles of parsimony.
GO:1901137 carbohydrate derivative biosynthetic process
COMPLETE DEPRECATE OVERLY_COMPLEX NONE 0 0.05
ARBA00026372
YAML
This rule predicts GO:0051998 (protein carboxyl O-methyltransferase activity) using 5 condition sets that identify two mechanistically distinct enzyme families: isoprenylcysteine carboxyl methyltransferases (ICMT) and protein-L-isoaspartate methyltransferases (PIMT). These enzymes share a common chemistry (methylating carboxyl groups on proteins) but differ fundamentally in substrate specificity, cellular location, biological function, and mechanism.
GO:0051998 protein carboxyl O-methyltransferase activity
COMPLETE DEPRECATE OVERLY_COMPLEX CONTRADICTED 5 0.95
ARBA00026480
YAML
This rule assigns GO:0010605 (negative regulation of macromolecule metabolic process) to proteins containing any of 67 different InterPro domains across 343 condition sets. The rule spans an extraordinarily broad taxonomic range including bacteria, plants, fungi, and animals.
GO:0010605 negative regulation of macromolecule metabolic process
COMPLETE DEPRECATE OVERLY_COMPLEX CONTRADICTED 0 0.95
ARBA00026572
YAML
Mega-rule with 781 condition sets applying the extremely broad GO term "nucleobase-containing compound metabolic process" to diverse protein families including DNA polymerases, tRNA synthetases, nucleotide kinases, and cofactor biosynthesis enzymes
GO:0006139 nucleobase-containing compound metabolic process
COMPLETE DEPRECATE OVERLY_COMPLEX CONTRADICTED 0 0.00
ARBA00026647
YAML
Mega-rule predicting GO:0010629 negative regulation of gene expression across 131 condition sets covering diverse RNA-binding proteins, chromatin modifiers, nucleases, and regulatory complexes including Dicer, PIWI, SET domain histone methyltransferases, sirtuins, pumilio repeats, and zinc finger proteins COMPLETE DEPRECATE OVERLY_COMPLEX MODERATE 0 0.10
ARBA00026698
YAML
Extremely complex rule with 26 condition sets predicting vacuolar transport (GO:0007034) based on diverse protein domains including SNARE domains, ENTH domains, Sec1-like domains, and unknown function domains. The rule attempts to capture multiple distinct vacuolar transport mechanisms under a single broad GO annotation across diverse taxonomic groups.
GO:0007034 vacuolar transport
COMPLETE DEPRECATE OVERLY_COMPLEX CONTRADICTED 6 0.05
ARBA00026708
YAML
Rule predicting peroxisome organization (GO:0007031) based on multiple InterPro domains and CATH FunFam classifications across different taxonomic groups. The rule uses 10 condition sets targeting various peroxisomal proteins including peroxins and peroxisome division factors.
GO:0007031 peroxisome organization
COMPLETE MODIFY OVERLY_COMPLEX MODERATE 10 0.45
ARBA00026799
YAML
Predicts NAD+ kinase activity (GO:0003951) for proteins containing NAD kinase domains across 6 condition sets covering bacterial, eukaryotic, and mitochondrial variants
GO:0003951 NAD+ kinase activity
COMPLETE ACCEPT ACCEPTABLE STRONG 6 0.85
ARBA00026991
YAML
ARBA rule with 83 condition sets targeting diverse metabolic enzymes (glycolysis, TCA cycle, electron transport, glycogen metabolism) for broad energy metabolism annotation GO:0015980 COMPLETE DEPRECATE OVERLY_COMPLEX CONTRADICTED 0 0.90
ARBA00027164
YAML
Mega-rule with 51 condition sets that broadly annotates diverse RNA-associated proteins with the general GO term RNA catabolic process (GO:0006401) COMPLETE DEPRECATE OVERLY_COMPLEX CONTRADICTED 0 0.95
ARBA00027169
YAML
Annotates proteins containing Sm or Sm-like domains with GO:0120114 "Sm-like protein family complex" using 28 condition sets covering classical Sm proteins, Lsm proteins, and various spliceosomal components across different taxonomic groups COMPLETE DEPRECATE OVERLY_COMPLEX MODERATE 0 0.20
ARBA00027389
YAML
ARBA rule that predicts "cell wall biogenesis" (GO:0042546) for proteins matching any of 29 diverse condition sets spanning cell wall enzymes, polyketide synthases, protein kinases, GTPases, and other functionally unrelated families across multiple taxonomic groups COMPLETE DEPRECATE OVERLY_COMPLEX CONTRADICTED 0 0.95
ARBA00027399
YAML
Predicts serine-type peptidase activity (GO:0008236) across a highly diverse collection of 36 condition sets encompassing serine proteases from multiple functional families including blood coagulation factors, complement system proteases, digestive enzymes, proprotein convertases, and bacterial quality control proteases. COMPLETE MODIFY OVERLY_COMPLEX MODERATE 0 0.60
ARBA00027430
YAML
An overly complex rule with 89 condition sets that attempts to predict "alcohol metabolic process" (GO:0006066) across diverse taxonomic groups and enzyme families. The rule combines various unrelated enzyme families including cytochrome P450s, dehydrogenases, kinases, and metabolic enzymes.
GO:0006066 alcohol metabolic process
COMPLETE DEPRECATE OVERLY_COMPLEX CONTRADICTED 0 0.10
ARBA00027624
YAML
A highly complex rule with 304 condition sets that predicts GO:0031090 (organelle membrane) for proteins containing diverse domain families across 406 CATH FunFam domains, 68 InterPro domains, and spanning 63 taxonomic groups from genus to kingdom level.
GO:0031090 organelle membrane
COMPLETE DEPRECATE OVERLY_COMPLEX CONTRADICTED 0 0.95
ARBA00027651
YAML
Mega-rule attempting to annotate all phosphoprotein phosphatases with GO:0004721 (phosphoprotein phosphatase activity) using 74 diverse condition sets covering tyrosine-protein phosphatases, serine/threonine phosphatases, dual-specificity phosphatases, and related phosphatases across all domains of life COMPLETE DEPRECATE OVERLY_COMPLEX MODERATE 0 0.10
ARBA00027723
YAML
A massive rule with 1094 condition sets that attempts to annotate the entire transferase enzyme class (EC 2) using GO:0016740 (transferase activity). The rule encompasses diverse transferase families including glycosyltransferases, acetyltransferases, protein kinases, ubiquitin-conjugating enzymes, and others, but also erroneously includes non-transferase domains like PAS signaling domains.
GO:0016740 transferase activity
COMPLETE DEPRECATE OVERLY_COMPLEX WEAK 0 0.95
ARBA00027853
YAML
Assigns GO:0006720 "isoprenoid metabolic process" to proteins matching any of 94 alternative condition sets built from InterPro entries, CATH FunFams, PANTHER families and taxon constraints. The GO term is in the right branch for most of the rule: 64 of the 94 sets identify genuine isoprenoid enzymes - terpene synthases and cyclases, the MVA and MEP precursor pathways, prenyl diphosphate synthases, carotenoid backbone and cleavage enzymes, gibberellin and ABA oxidases, named retinoid enzymes and JHAMT. Thirteen sets should be removed - twelve off-target (LDLR/LRP1, CYP2C9, CYP83B1, ADH5, ADH7, hormone-sensitive lipase, PNPLA2, phenylalanine aminomutase, bare UGT and Rossmann FunFams) plus one ALDH FunFam conjunction that appears unsatisfiable - one set is mixed and needs splitting, and sixteen cannot be audited from their identifiers. The rule's most serious defect is not biological: the 8,974 annotations it currently emits cannot be reproduced from its published condition sets.
GO:0006720 isoprenoid metabolic process
COMPLETE MODIFY OVERLY_COMPLEX MODERATE 94 0.80
ARBA00027994
YAML
Extremely complex rule attempting to capture all chromatin remodeling activities across eukaryotes using 134 condition sets spanning ATP-dependent remodelers, histone-modifying enzymes, chromatin assembly factors, and pioneer transcription factors.
GO:0006338 chromatin remodeling
COMPLETE SPLIT OVERLY_COMPLEX MODERATE 0 0.60
ARBA00028131
YAML
An extremely complex mega-rule with 41 condition sets assigning GO:0004175 (endopeptidase activity) to proteins across virtually all major endopeptidase families including aspartic proteases (pepsin family), cysteine proteases (papain/cathepsin families), serine proteases (subtilisin family), and metalloproteases (aminopeptidase family). While biologically sound, the rule represents a maintenance nightmare due to its extreme complexity and loses functional specificity by applying a single broad term to mechanistically distinct protease families. COMPLETE MODIFY OVERLY_COMPLEX STRONG 0 Not recorded
ARBA00028334
YAML
ARBA rule that annotates proteins with "regulation of gene expression" (GO:0010468) based on presence of various InterPro domains across different taxonomic lineages. This rule contains 930 condition sets with 267 unique InterPro domains, making it extraordinarily complex and impossible to validate comprehensively.
GO:0010468 regulation of gene expression
COMPLETE DEPRECATE OVERLY_COMPLEX NONE 0 0.95
ARBA00028538
YAML
Assigns GO:0046467 to proteins matching any of 51 alternative condition sets built from 14 InterPro entries, 51 CATH FunFams and 39 taxon constraints. GO obsoleted GO:0046467 ("membrane lipid biosynthetic process") on 2025-12-09, replacing it with GO:0008610 lipid biosynthetic process, so the rule now emits no annotations at all - yet UniProt still serves it, and its last modification date of 2025-12-15 is six days after the obsoletion. The antecedents are heterogeneous: at most 26 of the 51 sets describe enzymes that build a membrane lipid, while the rest capture sphingolipid hydrolases, lipid A and GPI remodelling enzymes, GPI/dolichol-donor assembly, non-diagnostic glycosyltransferase families, and four mechanistically unrelated proteins reached through promiscuous folds (a PH-domain protein kinase, phosphoglycerate kinase, 5-aminolevulinate synthase, and a lipid-binding lipoprotein).
GO:0046467 obsolete membrane lipid biosynthetic process
COMPLETE DEPRECATE OVERLY_COMPLEX WEAK 51 0.95
ARBA00028584
YAML
Rule predicts GO:0005778 (peroxisomal membrane) localization for nine distinct protein families: fungal DysF-domain peroxins (PEX23-32), peroxisomal ABC transporters (ABCD1, ABCD3), peroxisomal import machinery (PEX13, PEX14), RING finger peroxins (PEX12), the peroxisomal solute carrier SLC25A17/PMP34, the autophagy cargo receptor NBR1, and peroxisomal 2,4-dienoyl-CoA reductase (DECR2). Each condition set uses different domain signatures with varying taxonomic restrictions.
GO:0005778 peroxisomal membrane
COMPLETE MODIFY ACCEPTABLE STRONG 9 0.80
ARBA00028655
YAML
Assigns GO:0006661 "phosphatidylinositol biosynthetic process" to proteins matching any of 25 alternative condition sets built from InterPro entries, CATH FunFams and taxon constraints. Only two of the 25 sets (CS18, CS19) identify the enzyme that actually makes phosphatidylinositol - CDP-diacylglycerol--inositol 3-phosphatidyltransferase (CDIPT/PIS, EC 2.7.8.11). The remaining 23 sets capture phosphoinositide kinases, phosphoinositide phosphatases, non-catalytic PI3K/VPS34 regulatory subunits, GPI-anchor pathway enzymes, DPM1, and two promiscuous structural folds - all of which act on, consume or are merely adjacent to PI rather than synthesising it.
GO:0006661 phosphatidylinositol biosynthetic process
COMPLETE SPLIT OVERLY_COMPLEX CONTRADICTED 25 0.95
ARBA00028665
YAML
Overly complex rule with 174 condition sets attempting to annotate diverse protein classes (transcription factors, kinases, adhesion molecules, structural proteins) with the broad GO term "cell development" (GO:0048468) COMPLETE DEPRECATE OVERLY_COMPLEX WEAK 0 0.95
ARBA00028985
YAML
A highly complex mega-rule with 357 condition sets that annotates diverse RNA-binding and RNA-processing proteins with the overly broad GO term "RNA metabolic process" (GO:0016070). The rule indiscriminately captures functionally distinct protein families including aminoacyl-tRNA synthetases, ribonucleases, RNA helicases, and RNA-binding proteins, providing minimal functional specificity. COMPLETE DEPRECATE OVERLY_COMPLEX CONTRADICTED 0 0.05
ARBA00029057
YAML
Overly broad ARBA rule with 89 condition sets targeting diverse protein families (metabolic enzymes, structural proteins, transport proteins, kinases) for annotation with GO:0065003 protein-containing complex assembly COMPLETE DEPRECATE OVERLY_COMPLEX WEAK 0 0.95
ARBA00035048
YAML
Annotates diverse spliceosomal complex components including snRNPs, helicases, and associated factors across multiple taxonomic groups using 17 distinct condition sets COMPLETE MODIFY OVERLY_COMPLEX STRONG 0 0.60
ARBA00035068
YAML
Assigns phosphatidylinositol-3-phosphate phosphatase activity (GO:0004438) to proteins containing myotubularin-related functional family domains, with condition set 1 targeting MTM1-related domains in Primates and condition set 2 targeting MTMR4-related domains across all Eukaryotes COMPLETE MODIFY ACCEPTABLE STRONG 2 0.75
ARBA00043370
YAML
Rule assigns GO:0140115 "export across plasma membrane" to proteins matching 12 complex condition sets covering diverse transporter families including sugar transporters, ABC transporters, MFS transporters, P-type ATPases, and antiporters across various taxonomic groups
GO:0140115 export across plasma membrane
COMPLETE DEPRECATE OVERLY_COMPLEX CONTRADICTED 0 0.90
ARBA00045851
YAML
Annotates MORN repeat-containing proteins, specifically MORN3 family members, with function involving p53 regulation through assembly of a suppression complex that tethers SIRT1 and MDM2 COMPLETE MODIFY ACCEPTABLE MODERATE 2 0.40
ARBA00047230
YAML
Predicts phosphatidylinositol-3,5-bisphosphate 3-phosphatase activity for proteins containing myotubularin-like domains and/or specific CATH functional families, with taxonomic restrictions to Primates or Eukaryota COMPLETE MODIFY OVERLY_COMPLEX STRONG 3 0.65
ARBA00047239
YAML
Rule predicts GO:0032206 (positive regulation of telomere maintenance) using 9 condition sets covering diverse protein families: TRiC chaperonin subunits, TRF2 (shelterin), ATM kinase, PARP enzymes, RTEL1 helicase, MAPK, RuvB-like helicase, and SLX4 endonuclease. The rule combines OR logic across mechanistically distinct families involved in telomere biology, but contains significant redundancy, questionable domain choices, and taxonomic errors.
GO:0032206 positive regulation of telomere maintenance
COMPLETE MODIFY OVERLY_COMPLEX MODERATE 9 0.35
ARBA00047244
YAML
Rule predicting amide catabolic process (GO:0043605) based on four condition sets: (1) urease domains (beta/gamma subunits and active site) across all organisms, (2) PM20D1 FunFams in Eukaryota, (3) ureide pathway hydrolases in Viridiplantae, and (4) (S)-ureidoglycine aminohydrolase in Streptophyta. The rule captures mechanistically diverse enzymes that share the common chemistry of amide bond hydrolysis but serve distinct biological functions.
GO:0043605 amide catabolic process
COMPLETE DEPRECATE OVERLY_COMPLEX STRONG 4 0.80
ARBA00049204
YAML
Rule annotates superoxide dismutase activity (EC 1.15.1.1) using 24 condition sets spanning all domains of life. While the biological target is correctly identified and universally conserved, the rule exhibits extreme complexity that exceeds manageable thresholds, making analysis and maintenance intractable.
EC:1.15.1.1 2 superoxide + 2 H(+) = H2O2 + O2
COMPLETE MODIFY OVERLY_COMPLEX STRONG 9 0.95
ARBA00085337
YAML
Predicts GO:0045454 "cell redox homeostasis" for proteins across 9 condition sets covering diverse redox-related protein families including nitric oxide synthases, thioredoxins, glutathione reductases, peroxiredoxins, and other oxidative stress response proteins with taxonomic restrictions
GO:0045454 cell redox homeostasis
IN_PROGRESS MODIFY OVERLY_COMPLEX WEAK 9 0.50
ARBA00085883
YAML
Rule predicting proteoglycan catabolic process (GO:0030167) based on four condition sets: (1) beta-galactosidase in Eukaryota, (2) alpha-L-iduronidase, (3) beta-hexosaminidase in Mus, and (4) N-acetylglucosamine-6-sulfatase in Metazoa. These enzymes are lysosomal hydrolases involved in sequential degradation of glycosaminoglycan (GAG) chains that constitute proteoglycans. However, beta-galactosidase and beta-hexosaminidase are primarily known for ganglioside degradation, raising questions about annotation specificity.
GO:0030167 proteoglycan catabolic process
COMPLETE MODIFY REDUNDANT STRONG 4 0.60
ARBA00086620
YAML
Rule predicts sexual sporulation resulting in cellular spore formation based on 5 condition sets containing diverse protein families (kinases, trafficking proteins, cell wall enzymes, motor proteins) across eukaryotic taxa COMPLETE DEPRECATE OVERLY_COMPLEX CONTRADICTED 5 0.90
ARBA00087037
YAML
Predicts laminin-121 trimer (GO:0005608) for proteins containing three specific CATH FunFam domains: Cadherin EGF LAG seven-pass G-type receptor (2.10.25.10:FF:000011), Laminin subunit beta 1 (2.10.25.10:FF:000065), and netrin-4 isoform X2 (2.10.25.10:FF:000333) COMPLETE DEPRECATE OVERLY_COMPLEX CONTRADICTED 1 0.90
ARBA00088058
YAML
Rule predicts GO:0051010 (microtubule plus-end binding) for proteins containing CAP-Gly domains (CLIP proteins), EB/RP family proteins, CLASP proteins, or CKAP5, in five condition sets with taxon-specific targeting across Glires, Primates, Fungi, and broader Eukaryota.
GO:0051010 microtubule plus-end binding
COMPLETE ACCEPT REDUNDANT STRONG 5 0.85
ARBA00088072
YAML
Deprecated/non-existent ARBA rule allegedly applying GO:0160103 (tRNA (guanine(26)-N2/guanine(27)-N2)-dimethyltransferase activity) to TRM1/Trm1 enzymes
GO:0160103 tRNA (guanine(26)-N2/guanine(27)-N2)-dimethyltransferase activity
COMPLETE DEPRECATE OVERLY_COMPLEX CONTRADICTED 0 0.95
ARBA00089174
YAML
Rule predicting adaptive thermogenesis (GO:1990845) based on two alternative condition sets: PM20D1 FunFams in Eukaryota, and TRPV1 FunFams in Mus. The rule captures two mechanistically distinct pathways to thermogenic regulation.
GO:1990845 adaptive thermogenesis
COMPLETE MODIFY REDUNDANT STRONG 2 0.85
ARBA00089175
YAML
This rule predicts GO:1990849 (vacuolar localization) for eukaryotic proteins containing two specific CATH FunFam domains: 1.20.58.900:FF:000013 and 2.30.29.30:FF:000315, both associated with pleckstrin homology domain-containing family M member 1 (PLEKHM1). The rule targets the eukaryotic domain (NCBITaxon:2759).
GO:1990849 vacuolar localization
COMPLETE ACCEPT ACCEPTABLE STRONG 1 0.80
ARBA00089176
YAML
This rule predicts GO:1990840 (response to lectin) for primate proteins using two distinct condition sets. Condition set 1 requires non-specific serine/threonine protein kinase domains (FunFams 1.10.510.10:FF:000011 and 3.30.200.20:FF:000069) in Primates (NCBITaxon:9443). Condition set 2 requires E1A binding protein p300 domains (FunFams 1.10.246.20:FF:000001 and 1.20.1020.10:FF:000001) in Catarrhini (NCBITaxon:9526, Old World monkeys and apes).
GO:1990840 response to lectin
COMPLETE DEPRECATE OVERLY_COMPLEX WEAK 2 0.25
ARBA00089180
YAML
Rule predicts GO:1990858 "cellular response to lectin" based on two disjoint condition sets: (1) two serine/threonine protein kinase domains in Primates, and (2) two E1A binding protein p300 acetyltransferase domains in Catarrhini (Old World primates). Analysis reveals complete disjunction between condition sets (Jaccard=0.0), complete redundancy within condition set 2 (Jaccard=1.0), and very low coverage of the predicted GO term (3-40% containment, 3-4% Jaccard for all domain-GO pairs).
GO:1990858 cellular response to lectin
COMPLETE DEPRECATE OVERLY_COMPLEX CONTRADICTED 2 0.95
ARBA00089391
YAML
ARBA00089391 predicts spermidine transmembrane transport (GO:1903711) for fungal proteins containing either MFS multidrug transporter domains (in Taphrinomycotina) or urea active transporter domains (in all Fungi). The rule combines taxonomic restrictions with functionally distinct transporter families.
GO:1903711 spermidine transmembrane transport
COMPLETE DEPRECATE OVERLY_COMPLEX WEAK 2 0.15
ARBA00089395
YAML
Predicts spermine transmembrane transport function for proteins containing CATH FunFam 1.20.1250.20:FF:000011 domain in Taphrinomycotina fungi COMPLETE DEPRECATE PARSIMONIOUS WEAK 1 0.10
ARBA00090047
YAML
This rule annotates proteins containing the SepA cytokinesis protein domain (CATH FunFam 1.20.58.2220:FF:000006) in Taphrinomycotina fungi with the highly specific GO term GO:1904498 (protein localization to mitotic actomyosin contractile ring). The rule targets a well-characterized cytokinesis regulator but applies an extremely specific localization annotation that requires strong experimental validation. COMPLETE MODIFY PARSIMONIOUS MODERATE 1 0.70
ARBA00090252
YAML
Rule annotates ribosomal protein components (L40, L10a) in fungi with nuclear export of ribosomal large subunit. Contains two condition sets targeting ubiquitin-ribosomal protein fusions and ribosomal proteins in fungal taxa. COMPLETE DEPRECATE REDUNDANT CONTRADICTED 2 1.00
ARBA00090718
YAML
Rule assigns (S)-2-hydroxyglutarate dehydrogenase activity (GO:0047545) to proteins containing CATH FunFam 3.40.50.720:FF:000041 (D-3-phosphoglycerate dehydrogenase domain) COMPLETE DEPRECATE PARSIMONIOUS CONTRADICTED 1 0.90
ARBA00092121
YAML
Annotates fungal proteins in CATH FunFam 3.10.20.30:FF:000003 (Developmentally-regulated GTP-binding protein 1) with GO:1903833 (positive regulation of cellular response to amino acid starvation). Currently covers 0 proteins. COMPLETE DEPRECATE ACCEPTABLE NONE 1 0.95
ARBA00095398
YAML
Deprecated ARBA rule that inappropriately annotated ribosomal protein L12.1/L12A with ribophagy function COMPLETE DEPRECATE OVERLY_COMPLEX CONTRADICTED 0 1.00

Generated from cached review YAML by just rules-index.