MetaboLights MTBLS404 → GO BP enrichment
Input: MetaboLights MTBLS404
Generated by go_bp_enrichment.py — GO
biological-process ORA via the enzyme/gene layer: metabolite → Rhea
reaction → human Swiss-Prot enzyme (UniProt) → GO BP, with is_a/part_of
closure and a one-sided hypergeometric test (BH-FDR). Live + cached.
- Foreground: 467 human enzymes catalysing reactions that involve the study
metabolites (70 metabolites mapped to reactions). - Background: 4052 human reviewed metabolic enzymes with ≥1 GO BP term.
- Currency/cofactor metabolites: excluded by Rhea reaction-degree ≥ 500 (1 of the study's metabolites dropped: CHEBI:16048).
Top enriched GO biological processes
| GO BP term | k/n | K/N | Fold | FDR |
|---|---|---|---|---|
| GO:0015849 organic acid transport | 94/467 | 195/4052 | 4.2× | 1.3e-36 |
| GO:0046942 carboxylic acid transport | 94/467 | 195/4052 | 4.2× | 1.3e-36 |
| GO:0019752 carboxylic acid metabolic process | 168/467 | 607/4052 | 2.4× | 7.0e-31 |
| GO:0043436 oxoacid metabolic process | 170/467 | 627/4052 | 2.4× | 3.3e-30 |
| GO:0006520 amino acid metabolic process | 81/467 | 211/4052 | 3.3× | 7.9e-23 |
| GO:0044281 small molecule metabolic process | 227/467 | 1117/4052 | 1.8× | 2.2e-22 |
| GO:1905039 carboxylic acid transmembrane transport | 49/467 | 94/4052 | 4.5× | 4.9e-20 |
| GO:0006865 amino acid transport | 46/467 | 92/4052 | 4.3× | 9.0e-18 |
| GO:0043648 dicarboxylic acid metabolic process | 42/467 | 78/4052 | 4.7× | 9.9e-18 |
| GO:0015711 obsolete organic anion transport | 23/467 | 26/4052 | 7.7× | 8.8e-17 |
| GO:0015718 monocarboxylic acid transport | 45/467 | 97/4052 | 4.0× | 7.7e-16 |
| GO:0003333 amino acid transmembrane transport | 37/467 | 68/4052 | 4.7× | 9.2e-16 |
| GO:0015804 neutral amino acid transport | 27/467 | 40/4052 | 5.9× | 1.5e-14 |
| GO:0170033 L-amino acid metabolic process | 48/467 | 121/4052 | 3.4× | 1.1e-13 |
| GO:0015740 C4-dicarboxylate transport | 19/467 | 22/4052 | 7.5× | 2.3e-13 |
| GO:0044282 small molecule catabolic process | 77/467 | 279/4052 | 2.4× | 2.5e-12 |
| GO:0089718 amino acid import across plasma membrane | 23/467 | 35/4052 | 5.7× | 5.9e-12 |
| GO:0170039 proteinogenic amino acid metabolic process | 45/467 | 119/4052 | 3.3× | 5.9e-12 |
| GO:0046395 carboxylic acid catabolic process | 57/467 | 180/4052 | 2.7× | 1.5e-11 |
| GO:0006835 dicarboxylic acid transport | 26/467 | 48/4052 | 4.7× | 7.4e-11 |
| GO:0015816 glycine transport | 13/467 | 13/4052 | 8.7× | 7.7e-11 |
| GO:0006099 tricarboxylic acid cycle | 18/467 | 24/4052 | 6.5× | 8.9e-11 |
| GO:0008202 steroid metabolic process | 52/467 | 164/4052 | 2.8× | 1.6e-10 |
| GO:0046394 carboxylic acid biosynthetic process | 59/467 | 206/4052 | 2.5× | 5.6e-10 |
| GO:0006103 2-oxoglutarate metabolic process | 14/467 | 16/4052 | 7.6× | 7.0e-10 |
| GO:0015721 bile acid and bile salt transport | 18/467 | 27/4052 | 5.8× | 1.9e-09 |
| GO:0015807 L-amino acid transport | 26/467 | 54/4052 | 4.2× | 1.9e-09 |
| GO:0044283 small molecule biosynthetic process | 83/467 | 357/4052 | 2.0× | 3.5e-09 |
| GO:0018126 protein hydroxylation | 11/467 | 11/4052 | 8.7× | 4.4e-09 |
| GO:0032328 alanine transport | 13/467 | 15/4052 | 7.5× | 4.5e-09 |
Where this sits
This completes the bridge from metabolomics to GO biological process by
routing through the curated enzyme/gene annotations (the layer GO-CAM also
builds on). It is organism-specific (human) by construction.
Compare with GO molecular-function enrichment (activities) on the same metabolites.